中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3518-3521.doi: 10.3969/j.issn.1673-8225.2011.19.022

• 干细胞与中医药 stem cells and traditional Chinese medicine • 上一篇    下一篇

三氧化二砷逆转HL60/ADR细胞耐药的研究

黄  明1，2，孟凡义1，蔡艳霞1，王治香1，夏丽华2，陈嘉斌2   

  1. 1南方医科大学南方医院血液科，广东省广州市  510515
    2广东省职业病防治院，广东省广州市  510300
  • 收稿日期:2011-02-17 修回日期:2011-04-02 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 孟凡义，硕士，教授，南方医科大学南方医院血液科，广东省广州市 510515 mengfu@medmail.com.cn
  • 作者简介:黄明☆，女，1980年生，湖南省平江县人，汉族，2010年南方医科大学毕业，博士，现在广东省职业病防治院工作，主治医师，主要从事血液病研究。 echo.echo@126.com
  • 基金资助:

    广东省科技计划项目(2007B031515005)。

Effect of arsenic trioxide on multi-drug resistance of HL60/ADR cells

Huang Ming1,2, Meng Fan-yi1, Cai Yan-xia1, Wang Zhi-xiang1, Xia Li-hua2, Chen Jia-bin2   

  1. 1Hematology Department, Nanfang Hospital of Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Guangdong Prevention and Treatment Center for Occuptional  Diseases, Guangzhou  510300, Guangdong Province, China
  • Received:2011-02-17 Revised:2011-04-02 Online:2011-05-07 Published:2011-05-07
  • Contact: Meng Fan-yi, Master, Professor, Hematology Department, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China mengfu@medmail.com.cn
  • About author:Huang Ming☆, Doctor, Attending physician, Hematology Department,Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China; Guangdong Prevention and Treatment Center for Occuptional Diseases, Guangzhou 510300, Guangdong Province, China echo.echo@126.com
  • Supported by:

    Guangdong Science and Technology Planning Project, No. 2007B031515005*

PDF

380

被引次数

14

摘要：

背景：临床应用三氧化二砷(As2O3)治疗复发难治性急性早幼粒细胞白血病已获得较好效果。
目的：探讨As2O3逆转急性髓系白血病细胞株HL60/ADR耐药的作用及其机制。
方法：以HL60/ADR细胞为研究对象，分为空白对照组和As2O3组，空白对照组不加任何药物培养，As2O3组加入48 h IC50 As2O3进行培养。
结果与结论：As2O3能提高HL60/ADR细胞摄取阿霉素水平，降低HL60/ADR细胞多药耐药相关蛋白1表达率(P < 0.05)，能够促进HL60/ADR细胞凋亡，细胞早期和晚期凋亡率均高于空白对照组(P < 0.01)，并随时间增加而提高。可减低细胞IκBα、p65、抗凋亡蛋白Bcl-2表达，使促凋亡蛋白Bax，断裂的Caspase-3、Caspase-9、PARP表达升高，说明As2O3能够逆转HL60/ADR细胞耐药，与其上述变化有关。

关键词: 三氧化二砷, 急性髓系白血病, 多药耐药, 机制, 凋亡

Abstract:

BACKGROUND: The treatment of arsenic trioxide (As2O3) on acute promyelocytic leukemia (APL) has acquired good effect.
OBJECTIVE: To investigate the effect and mechanism of As2O3 on reversing multidrug resistance of HL60/ADR cell line.
METHODS: HL60/ADR cells were divided into control group and As2O3 group. Cells of control group were incubated with nun-drug, those of As2O3 group incubated with 48-hour IC50 As2O3.
RESULTS AND CONCLUSION: As2O3 could enhance the intracellular accumulation of DNR and decrease the expression of MRP1 in HL60/ADR cells (P < 0.05). At the same time, the early and late apoptosis rate of HL60/ADR cells both increased significant with time-dependently, which was higher than that in the control group (P < 0.01). As2O3 could also elevate the expression of bax, caspase-3, caspase-9, PARP, and decelerate the expression of Bcl-2, NF-κB p65, p-p65.

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