中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (19): 3522-3524.doi: 10.3969/j.issn.1673-8225.2011.19.023

• 干细胞培养与分化 stem cell culture and differentiation • 上一篇    下一篇

不同pH值水平下脂肪间充质干细胞的存活和增殖

吴  坤1，2，李  放1，马  健1，2，李进珍1，2   

  1. 1解放军北京军区总医院骨科，北京市  100700
    2山西医科大学第二临床学院，山西省太原市  030001
  • 收稿日期:2010-12-08 修回日期:2011-04-09 出版日期:2011-05-07 发布日期:2011-05-07
  • 通讯作者: 李放，主任医师，教授，北京军区总医院骨科，主要从事骨外科学、脊柱外科学研究，北京军区总医院骨科，北京市 100700 fang16722@vip.sina.com
  • 作者简介:吴坤★，男，1985年生，河南省安阳市人，汉族，山西医科大学在读硕士，医师，主要从事椎间盘的组织工程学研究。 koalaxxmc123@163.com

Effect of pH levels in the survival and proliferation of adipose derived mesenchymal stem cells

Wu Kun1,2, Li Fang1, Ma Jian1,2, Li Jin-zhen1,2   

  1. 1Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100700, China
    2Second Clinical Medical College, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2010-12-08 Revised:2011-04-09 Online:2011-05-07 Published:2011-05-07
  • Contact: Li Fang, Professor, Chief physician, Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China fang16722@vip.sina.com
  • About author:Wu Kun★, Studying for master’s degree, Physician, SDepartment of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China ; Second Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China koalaxxmc123@163.com

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363

被引次数

2

摘要：

背景：在退变的椎间盘内，pH值的下降对脂肪间充质干细胞有很大影响。
目的：在不同的pH值条件下体外培养脂肪间充质干细胞，观察其增殖及生存情况。
方法：分离兔脂肪间充质干细胞，在分别在pH 7.4，7.1，6.8，6.5条件下培养5 d后用锥虫蓝染色检测各组的细胞存活率，采用MTT法绘制细胞生长曲线。
结果与结论：相比其他3组，pH 6.5条件下脂肪间充质干细胞存活率最低(P < 0.05)，其细胞生长速度持续减慢。结果证实，pH 6.5的酸度培养条件可抑制脂肪间充质干细胞的活力和增殖。

关键词: 椎间盘退变, 脂肪间充质干细胞, pH值, 增殖, 活力, 组织构建

Abstract:

BACKGROUND: Decreased pH value has a great effect on adipose derived mesenchymal stem cells (ADSCs) in the degenerated intervertebral disc.
OBJECTIVE: To observe the survival and proliferation of cultured ADSCs under different pH conditions.  
METHODS: ADSCs were isolated from fat tissue of rabbits and cultured under four different pH conditions (pH 7.4, pH 7.1, pH 6.8, pH 6.5). Five days later, typan blue staining was used to detect cell viability and MTT method was applied to draw cell growth curve.
RESULTS: The cell viability in the pH 6.5 group was the lowest compared with that in the other three groups (P < 0.05). It is indicated that pH 6.5 can inhibit the viability and proliferation of ADSCs.

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