中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1174-1178.doi: 10.3969/j.issn.1673-8225.2011.07.008

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

增龄因素与压力侧牙周组织Wnt-1及护骨素的表达

许连静1，秦明群2，毛峻武2，黄声富3   

  1. 1 桂林医学院，广西壮族自治区桂林市  541004
    2 桂林医学院附属医院口腔科，广西壮族自治区桂林市  541001
    3 武汉大学口腔医院口腔正畸科，湖北省武汉市  430079
  • 收稿日期:2010-09-19 修回日期:2011-01-06 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 秦明群，硕士，教授，硕士生导师，桂林医学院附属医院口腔科，广西壮族自治区桂林市 541001 qmq@vip.sina. com
  • 作者简介:许连静★，女，1982年生，辽宁省阜蒙县人，汉族，桂林医学院在读硕士，主要从事口腔组织病理学方面的研究。 xulianjing825@ 163.com
  • 基金资助:

    广西卫生厅重点科研项目(编号：重200980)，课题名称：Wnt/DKK在正畸骨改建中对成骨细胞和破骨细胞的双向调节作用。

Age-related effect on Wnt-1 and osteoprotegerin expression in pressure side of periodontal tissue

Xu Lian-jing1, Qin Ming-qun2, Mao Jun-wu2, Huang Sheng-fu3   

  1. 1Guilin Medical College, Guilin  541004, Guangxi Zhuang Autonomous Region, China
    2Department of Stomatology, Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
    3Department of Orthodontics, Stomatological Hospital of Wuhan University, Wuhan  430079, Hubei Province, China
  • Received:2010-09-19 Revised:2011-01-06 Online:2011-02-12 Published:2011-02-12
  • Contact: Qin Ming-qun, Master, Professor, Master’s supervisor, Department of Stomatology, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China qmq@vip.sina.com
  • About author:Xu Lian-jing★, Studying for master’s degree, Guilin Medical College, Guilin 541004, Guangxi Zhuang Autonomous Region, China xulianjing825@163. com
  • Supported by:

    the Key Scientific Research Fund of Guangxi Health Bureau, No. 200980*

PDF

453

被引次数

4

摘要：

背景：护骨素在正畸牙骨改建中具有重要作用，Wnt-1与骨形成密切相关，但与正畸骨改建的相关性未见报道。
目的：比较增龄因素对大鼠正畸牙牙周组织Wnt-1及护骨素表达的影响。
方法：纳入雄性6，24周龄Wistar大鼠各40只，制备大鼠磨牙向近中移动的正畸牙移动模型，于牙齿移动1，3，7，14 d分别取材，采用RT-PCR及免疫组织化学方法检测压力侧牙周组织中Wnt-1及护骨素的表达。
结果与结论：护骨素在6，24周龄大鼠压力侧牙周组织中均有表达，其中3，7 d表达强于1，14 d，相同时间点，6周龄大鼠压力侧护骨素的表达强于24周龄大鼠。6，24周龄大鼠压力侧牙周组织中均未见Wnt-1 mRNA的表达，Wnt-1蛋白仅在6周龄大鼠牙齿移动3 d时有表达。说明增龄因素是影响正畸骨改建的重要因素，而Wnt-1似乎与正畸骨改建无相关性。

关键词: Wnt-1, 护骨素, 增龄因素, 牙槽骨改建, 口腔组织工程

Abstract:

BACKGROUND: Osteoprotegerin (OPG) plays an important role in bone remodeling during orthodontic tooth movement. Wnt-1 is closely related with bone formation, but Wnt-1 correlated with orthodontic bone remodeling has not been reported.
OBJECTIVE: To compare age-related effect on periodontal tissue Wnt-1 during orthodontic tooth movement in rats and osteoprotegerin expression.
METHODS: A total of 40 male Wistar rats (6-week-old) and 40 male Wistar rats (24-week-old) were included. Orthodontic tooth movement model of rat molar mesial movement was prepared, the model was drawn at 1, 3, 7, and 14 days tooth movement. The expression of Wnt-1 and OPG in pressure side of periodontal tissue was detected by RT-PCR and immunohistochemistry method.
RESULTS AND CONCLUSION: OPG was expressed at the pressure side of periodontal tissues, which was stronger on days 3 and 7 than days 1 and 14; at the same time point, the OPG expression of pressure side of rats (6-week-old) was stronger than that of 24-week-old rats. Wnt-1 was not expressed at the pressure side of periodontal tissue in rats (6-week-old, 24-week-old). Wnt-1 protein expression of 6-week-old rats was existed at 3 days after tooth movement. Age factor is an important factor in orthodontic bone remodeling; however, Wnt-1 seems no correlation with orthodontic bone remodeling.

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