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    18 May 2026, Volume 30 Issue 14 Previous Issue   
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    Structural design and finite element analysis of biodegradable zinc alloy bone plate based on regression analysis
    Zhang Tianwei, Han Xingyuan, Zhang Dianming, Li Ronghua, Zhao Dewei
    2026, 30 (14):  3485-3493.  doi: 10.12307/2025.581
    Abstract ( 11 )   PDF (1600KB) ( 0 )   Save
    BACKGROUND: In recent years, biodegradable zinc alloy bone plates could effectively solve the clinical problems such as stress shielding effect and secondary surgical removal. The main factors that affect the stress shielding of biodegradable bone plates include the structural design, material selection, and degradation rate. However, the relationship between the structural design and stress shielding effect of biodegradable zinc alloy bone plates is rarely studied, and there is a lack of scientific basis.
    OBJECTIVE: To explore the relationship between the structural design of biodegradable zinc alloy plates and the stress shielding effect.
    METHODS: Ordinary zinc-magnesium alloy plates were used to fix tibial fractures in New Zealand rabbits. The plates were removed 3, 6, 9, and 12 months after surgery, and the material degradation rate was analyzed. An orthogonal experiment was designed to compare the four parameters of plate thickness, screw hole diameter, arc diameter, and hole diameter with plate stress. Based on the constructed data set, a prediction model of the four parameters and zinc-magnesium alloy plate stress was established by regression analysis. The optimal parameters of plate design were obtained according to different constraints. Finite element analysis was used to compare the biomechanical properties of zinc-magnesium alloy plates and titanium alloy plates before and after optimization design in a rabbit tibial fracture model.
    RESULTS AND CONCLUSION: (1) After implantation in rabbits, the surface of the zinc-magnesium alloy plate showed obvious corrosion, and the degree of corrosion gradually deepened over time. Compared with before implantation, the degradation rates of the zinc-magnesium alloy plate were 11.5%, 17.9%, 21.8%, and 24.5% at 3, 6, 9, and 12 months after implantation, respectively. (2) The optimal theoretical structural parameters of the plate were obtained by regression model: plate thickness 1.1 mm, screw hole diameter 2.4 mm, hole diameter 0.6 mm, arc diameter 6.0 mm. (3) The results of finite element analysis showed that the maximum stresses of the zinc-magnesium alloy plate and titanium alloy plate before and after optimization were less than their yield strength under the composite working conditions. During the initial fixation, the displacement of the fracture end of the titanium alloy plate group before and after optimization was 0.08 mm and 0.12 mm, respectively. The displacement of the fracture end of the zinc-magnesium alloy plate group before and after optimization was 0.10 mm and 0.13 mm, respectively. After 3 months of degradation, the displacement of the fracture end of the zinc-magnesium alloy plate group before and after optimization was 0.11 mm and 0.15 mm, respectively. Both plates were theoretically safe for the treatment of rabbit tibial fractures. Compared with the titanium alloy plate, the zinc-magnesium alloy plate exhibited a lower stress shielding effect. With degradation, the stress shielding rates of the zinc-magnesium alloy plate group before and after optimization decreased by 27.56% and 27.66%, respectively. The results exhibit that the zinc-magnesium alloy plate provides mechanical support in the early stage, and reduces the stress shielding effect in the later stage as the material degraded, which is beneficial to bone healing.
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    Magnetocaloric antitumor and osteogenic properties of magnetic bioactive glass scaffolds
    Li Qingshan, Li Runmeng, Gao Yuyang, Han Gang, Chen Jiying, Guo Quanyi
    2026, 30 (14):  3494-3503.  doi: 10.12307/2026.630
    Abstract ( 11 )   PDF (3072KB) ( 0 )   Save
    BACKGROUND: With the development of tissue engineering, it is of great significance to design a bioactive scaffold that prevents tumor recurrence and possesses good osteogenic properties.
    OBJECTIVE: To evaluate the biocompatibility, magnetic thermal antitumor properties, and osteogenic differentiation potential of borosilicate bioactive glass scaffolds loaded with magnetic hydrogels.
    METHODS: Magnetic hydrogels were prepared by combining methacrylated gelatin with magnetic ferric oxide nanoparticles and were loaded onto borosilicate bioactive glass scaffolds to create magnetic bioactive glass scaffolds. The morphology and magnetothermal properties of the magnetic bioactive glass scaffolds were characterized. Rat bone marrow mesenchymal stem cells were cultured with borosilicate bioactive glass scaffolds and magnetic bioactive glass scaffold extracts, respectively. The biocompatibility of the scaffolds was evaluated by CCK-8 assay and live/death staining. After osteogenic induction, the osteogenic properties of the scaffolds were evaluated by alkaline phosphatase staining, alizarin red staining, and qRT-PCR detection of osteogenic gene expression. Human osteosarcoma cells were co-cultured with borosilicate bioactive glass scaffolds, magnetic bioactive glass scaffolds, and magnetic bioactive glass scaffolds under alternating magnetic field intervention. The anti-tumor properties of the scaffolds were evaluated by CCK-8 assay, live/death staining, and flow cytometry apoptosis detection.
    RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that the hydrogel carrying magnetic nanoparticles was stably loaded in the pores of the borosilicate bioactive glass scaffold. The hysteresis and magnetic thermal curves indicated that the magnetic bioactive glass scaffolds exhibited good magnetic thermal properties. (2) CCK-8 assay and live/dead staining the magnetic bioactive glass scaffold and the borosilicate bioactive glass scaffold had no obvious cytotoxicity and both promoted the proliferation of rat bone marrow mesenchymal stem cells, among which the magnetic bioactive glass scaffold had a more obvious promoting effect. Under osteogenic induction conditions, both groups of scaffolds could promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells and increase the mRNA expression of osteocalcin and Runx2, among which the magnetic bioactive glass scaffold had a more obvious promoting effect. (3) CCK-8 assay, live/death staining, and flow cytometry apoptosis detection showed that compared with the borosilicate bioactive glass scaffold and the magnetic bioactive glass scaffold, the magnetic bioactive glass scaffold under the intervention of alternating magnetic field could inhibit the proliferation activity of human osteosarcoma cells and induce cell apoptosis. The results showed that the magnetic bioactive glass scaffold had good magnetocaloric properties, osteogenic properties and anti-tumor properties.
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    Finite element analysis of mechanical properties of porous tantalum implants with different structures and porosities
    Wang Ruihao, Hu Xiaohua, Wang Yujiao, Linghu Min, Yang Xiaohong
    2026, 30 (14):  3504-3514.  doi: 10.12307/2026.631
    Abstract ( 8 )   PDF (3441KB) ( 0 )   Save
    BACKGROUND: Porous tantalum may be an ideal oral implant material to replace titanium and titanium alloys, but its optimal structure and porosity remain to be explored.
    OBJECTIVE: To identify the porosity of porous tantalum with a trabecular, diamond, and cubic structure that most closely matches the elastic modulus of the jawbone using finite element analysis. Among the three groups with the most optimal elastic modulus, we further explored the structure most conducive to reducing bone stress and promoting initial cell adhesion during implantation.
    METHODS: Using nTop software, we created models of the trabecular, diamond, and cubic structures with 60%, 70%, and 80% porosity, resulting in a total of nine groups. Ansys software was employed for static pressure simulation to record the deformation along the direction of applied force. The elastic modulus was calculated, and the optimal group for each structure was selected based on the closest match to the elastic modulus of the jawbone. A dental implant design with a porous scaffold in the middle and solid screw segments at the top and bottom was simulated for implantation, and the stress after implantation was simulated. The internal stress of the mandible under the stress after bone implantation of the three groups of optimal structure implants was analyzed to explore a group of porous scaffold structures that are most conducive to reducing the internal stress of the bone under similar elastic modulus. The three groups of optimal elastic modulus groups were simulated by fluid mechanics using Ansys software to explore the structure that is most conducive to early cell adhesion and osteogenesis.
    RESULTS AND CONCLUSION: (1) Static pressure simulations revealed that the diamond 60%, trabecular 70%, and cubic 80% groups had the closest elastic modulus to that of the mandibular cortical bone among the different porosities of each structure. Static pressure analysis under load after tantalum implant implantation showed that the cancellous stress of the trabecular 70% group was lower than that of the diamond 60% group and cubic 80% group, and the cortical stress of the diamond 60% group was higher than that of the cubic 80% group. (2) Fluid mechanics analysis showed that the volume of the low-velocity area near the scaffold of the cubic 80% group was the largest, the volume of the low-velocity area near the scaffold of the trabecular 70% group was slightly lower than that of the cubic 80% group, and the volume of the low-velocity area near the scaffold of the diamond 60% group was the smallest. After introducing discrete phase particles to simulate cell movement, it was found that although the volume of the low-velocity area near the scaffold of the trabecular 70% group was slightly smaller than that of the cubic 80% group, the low-velocity area near the scaffold had the most particles staying, which was most conducive to early cell adhesion and osteogenesis.
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    Three-dimensional finite element analysis of influence of different filling methods of the maxillary second premolar on root resistance
    Zhang Junjie, Gegentana
    2026, 30 (14):  3515-3523.  doi: 10.12307/2026.632
    Abstract ( 6 )   PDF (2070KB) ( 0 )   Save
    BACKGROUND: Previous studies on root canal filling with thermoplasticized gutta-percha and single-point technique have mainly focused on the density of root canal filling, while research on the root’s resistance to fracture is relatively scarce. Understanding the stress distribution and crack characteristics of the root under different filling methods is of great significance for improving the success rate of root canal treatment. 
    OBJECTIVE: To compare the maximum compressive load strength of the root of the maxillary second premolar after root canal filling with thermoplasticized gutta-percha vertical compaction and iRoot SP single-point technique, and to analyze the stress concentration areas, crack features, and overall fracture resistance of the root. 
    METHODS: (1) The discarded maxillary second premolars extracted for orthodontic reduction were selected and filled with thermoplasticized gutta-percha vertical compaction and iRoot SP single-point technique respectively. The in vitro teeth were subjected to vertical loading tests using an electronic universal testing machine to detect the location of root cracks and the maximum yield force and maximum fracture resistance of the root. (2) The maxillary second premolar original data were obtained by cone beam CT scanning, and three-dimensional reconstruction was performed using Mimics 21.0 and Geomagic 2021 software. SolidWorks 2021 was used for model assembly. The filling models made by thermoplasticized gutta-percha vertical compaction and iRoot SP single-point technique were established respectively. ANSYS Workbench software was used for mechanical analysis to analyze the maximum principal stress and stress concentration site of the root.
    RESULTS AND CONCLUSION: (1) Microscopic observation showed that cracks occurred in the buccal and lingual directions of the roots of both groups. The maximum yield force of the root in the thermoplasticized gutta-percha vertical compaction group was 336.4 N, and the fracture resistance was 1 124.6 N. In the iRoot SP single-point technique group, the yield force was 468.8 N, and the fracture resistance was 1 263.7 N. (2) Finite element analysis showed that with the increasing pressure value, the maximum principal stress values of the roots of the two groups showed an upward trend. The maximum principal stress value of the roots of the iRoot SP single-point technique group was always higher than that of the thermoplasticized gutta-percha vertical compaction group. The root stress of the thermoplasticized gutta-percha vertical compaction group was concentrated around the root canal filling material, and the root stress of the iRoot SP single-point technique group was mainly distributed around the gutta-percha tip. In the case of flattened root canals, the root stress concentration area and crack initiation position were mainly located in the buccal and lingual directions. The crack initiation position predicted by three-dimensional finite element simulation was highly consistent with the actual root crack position observed in the in vitro experiment, confirming the validity of the finite element model. (3) The study indicates that for the flattened root canals of maxillary second premolars, the iRoot SP single-point technique shows significant advantages in root fracture resistance and stress distribution.
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    Finite element analysis of stress distribution of Schneiderian membrane after maxillary sinus elevation and implantation under different bone quality conditions
    Du Xue, Luo Siyang, Feng Hongchao, Liu Jianguo, Luo Yi, Sun Jiangling, Chang Xingtao, Li Yuting, Wang Ruijie
    2026, 30 (14):  3524-3535.  doi: 10.12307/2026.654
    Abstract ( 5 )   PDF (2439KB) ( 0 )   Save
    BACKGROUND: There are many studies on the stress distribution of the Schneiderian membrane. However, domestic and international research on the stress analysis of the Schneiderian membrane mainly focuses on the stress-strain analysis during the maxillary sinus elevation surgery or of the isolated Schneiderian membrane, while the research on the stress-strain of the Schneiderian membrane during occlusion after maxillary sinus elevation and implant restoration is relatively rare.
    OBJECTIVE: To investigate the stress distribution, stress peak, and displacement of the Schneiderian membrane during occlusion after maxillary sinus elevation and implant restoration with different remaining bone volumes in the maxillary molar area and under different bone quality conditions using finite element analysis. 
    METHODS: Based on cone beam CT scan files of healthy adult volunteers, models of maxillary sinus lift and implants and related accessories were established. Four bone properties (class I-IV cancellous bone), two residual bone volumes in the maxillary first molar area (residual bone height 3 mm and 5 mm), three implant morphologies (large thread implant, cylindrical implant, and tapered implant) and three models after maxillary sinus lift [maxillary sinus lift without bone grafting or bone grafting but no bone formation after lift (S group), maxillary sinus lift with bone grafting around implant but no bone formation at the apex (T group), and maxillary sinus lift with bone grafting around implant and at the apex (U group)] were set. Finite element analysis was performed on the stress distribution, stress peak and displacement of Schneiderian membrane in each group of models under static load. 
    RESULTS AND CONCLUSION: (1) When the bone quality gradually changed from Class I to Class IV, the Von Mises stress peak and displacement peak of Schneiderian membrane in each group of models showed a gradual upward trend, reaching the maximum value in Class IV bone. When the remaining bone volume in the maxillary first molar area was 3 mm, the bone quality was Class IV, and large threaded implants were implanted, the Von Mises stress peak of Schneiderian membrane in the S group model was the largest (0.558 MPa) and the displacement peak was the largest (278 μm). When the bone quality gradually changed from Class I to Class IV, the displacement peak of Schneiderian membrane in each group of models showed a gradual upward trend. Under the same bone quality conditions, the displacement peak of Schneiderian membrane in the models implanted with cylindrical, conical, and large threaded implants showed the order of S group > T group > U group. (2) The results show that when the remaining bone volume was small and the bone density was poor, large threaded implants should be used with caution for maxillary sinus lift surgery. When selecting the implant system before maxillary sinus lift surgery, the state of the maxillary sinus mucosa should be taken into consideration before surgery, and the implant plan should be planned accordingly. The height, density and bone formation of the remaining bone volume and the bone formation in the maxillary sinus determined the stress peak and displacement peak of the occlusal force transmitted to the Schneiderian membrane after implant restoration. The patient's remaining bone volume and bone density should be fully considered during maxillary sinus lift surgery. The Schneiderian membrane should be peeled off as much as possible during surgery to increase the bone formation area. The cylindrical implant and the conical implant had no significant effect on the stress distribution and displacement deformation of the Schneiderian membrane after maxillary sinus lift implant restoration. When the remaining bone volume was less than 3 mm for maxillary sinus lift implant restoration, the use of bone transplant materials could significantly reduce the implant mobility and the stress on the Schneiderian membrane during occlusion after restoration. When the remaining bone volume in the maxillary molar area was greater than 5 mm, the peak stress of Schneiderian membrane was much lower than the average perforation tension of healthy Schneiderian membrane, regardless of whether there was bone formation around the implant and at the top.
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    Effect of root distal curvature with clear aligners on intruded maxillary central incisors analyzed using three-dimensional finite element analysis
    Ming Yu, Wang Peijun, Liu Xiaoyao, Li Jianan
    2026, 30 (14):  3536-3547.  doi: 10.12307/2026.128
    Abstract ( 14 )   PDF (5747KB) ( 0 )   Save
    BACKGROUND: For patients with root curvature of maxillary central incisors, clinicians often ignore the impact of root morphology changes on orthodontic treatment in patients with root curvature. There are few studies in this area.
    OBJECTIVE: To study the effects of different root distal curvature angles and diaphragm thicknesses with clear aligners on the impact of intruded maxillary central incisors by three-dimensional finite element analysis.
    METHODS: A three-dimensional finite element model of the left maxillary central incisors with different angles of apical 1/3 distal curvature (no curvature, 20° curvature, 40°curvature, and 60° curvature) and three kinds of various thicknesses of clear aligners (0.5, 0.75, and 1 mm) were constructed. ANSYS software was used to perform finite element analysis to compare the stress distribution characteristics of teeth and periodontal ligament, and the initial displacement trends of teeth and clear aligners when intruding 0.2 mm along the long axis of the left maxillary central incisor for different thicknesses of clear aligners. 
    RESULTS AND CONCLUSION: (1) The left maxillary central incisor showed a tipping movement trend during the intrusion, with the crown displacement more significant than the root displacement. The total displacement was inversely proportional to the root curvature angle and directly proportional to the diaphragm thickness. (2) As the root curvature angle increased, the crown showed an increase in displacement and extrusion in the mesiodistal direction, while the displacement in the labiolingual direction decreased. The root showed an increase in displacement in the mesiodistal direction, and both labiolingual displacement and intrusion decreased. The displacement of the crown and root in three dimensions was proportional to the thickness of the diaphragm. (3) The periodontal ligament and tooth Von-Mises stress was proportional to the root curvature angle and diaphragm thickness. The Von-Mises stress of the periodontal ligament was mainly distributed on the palatal side of the cervical tooth. Except for the thickness of the diaphragm of 0.5 and 0.75 mm, the root had no curvature, the thickness of the diaphragm was 0.5 mm, and the root was curvature by 20°, and the stress concentration of the root at the curvature occurred. (4) The displacement of the clear aligner was directly proportional to the angle of root curvature and inversely proportional to the thickness of the diaphragm, while the maximum displacement occurred at the labial edge of the depressed orthodontic appliance. (5) The root curvature of maxillary central incisors reduced the efficiency of tooth intrusion. When intruding a maxillary central incisor with a curved root, choosing a clear aligner with a thickness of 0.5 mm was recommended, and appropriately increasing the thickness of the appliance edge reduced the risk of the appliance coming off and lowered the probability of root resorption.

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    Preparation and mechanical properties of zirconia ceramic composites for stomatology
    Jin Hui, Chen Hui
    2026, 30 (14):  3548-3556.  doi: 10.12307/2025.900
    Abstract ( 20 )   PDF (2994KB) ( 0 )   Save
    BACKGROUND: 3% (molar percentage) yttria-stabilized tetragonal zirconia ceramics are prone to excessive wear of opposing teeth in practical applications, thereby increasing the risk of dentin exposure, causing toothache, pulp infection and other problems. Therefore, further improving the friction and wear properties of 3% yttria-stabilized tetragonal zirconia polycrystalline ceramics has become one of the hot topics in the current research of oral medical restorative materials.
    OBJECTIVE: To explore the effect of barium titanate doping ratio on the microstructure, mechanical properties and friction and wear properties of 3% yttria-stabilized tetragonal zirconia ceramics.
    METHODS: With 3% yttria-stabilized tetragonal zirconia ceramics as the main body, 3% yttria-stabilized tetragonal zirconia ceramic materials doped with barium titanate were prepared by high-temperature sintering method, in which the barium titanate doping amounts were 0%, 2%, 4%, and 6% of the ceramic powder mass, respectively. The lattice structure, mechanical properties, micromorphology and wear resistance of the four groups of materials were tested.
    RESULTS AND CONCLUSION: (1) X-ray diffraction results showed that with the increase of barium titanate doping, the proportion of monoclinic zirconia and barium titanate in the composite ceramics continued to increase, while the proportion of tetragonal zirconia continued to decrease. (2) Scanning electron microscopy results showed that there were a large number of pores on the surface of pure 3% yttria-stabilized tetragonal zirconia ceramics, and the surface density was low; the number of pores on the surface of 4% and 6% barium titanate doped ceramics decreased and the surface density increased. (3) With the increase of barium titanate doping, the density of ceramics continued to decrease, and the pores first decreased and then increased. Among them, the density of 6% barium titanate doped ceramics was the lowest, and the porosity of 4% barium titanate doped ceramics was the lowest. With the increase of barium titanate doping, the Vickers hardness and fracture toughness of ceramics continued to decrease, and the elastic modulus first increased and then decreased. Among them, the elastic modulus of 4% barium titanate doped ceramics was the largest. (4) The scratch test was carried out using a diamond with a diameter of 10 µm. The results showed that with the increase of the amount of barium titanate doped, the friction coefficient and scratch depth of the ceramics first decreased and then increased. Among them, the friction coefficient and scratch depth of 4% barium titanate doped ceramics were the lowest. The results of scanning electron microscopy showed that the four groups of ceramic samples had different degrees of breakage on both sides of the scratch. Among them, the scratch edge of the pure yttria-stabilized tetragonal zirconia ceramic was the most serious, and the scratch edge of the 4% barium titanate-doped ceramic was the lightest. (5) The results show that the 4% barium titanate-doped yttria-stabilized tetragonal zirconia ceramic composite material has high wear resistance and strength, and can maintain long-term stability.
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    Three-dimensional finite element analysis on distalization of orthodontic maxillary dentition
    Xie Lili, Zhang Hao, Xun Chunlei
    2026, 30 (14):  3557-3567.  doi: 10.12307/2026.108
    Abstract ( 16 )   PDF (3201KB) ( 0 )   Save
    BACKGROUND: Distalization of maxillary dentition is a commonly used non-extraction correction strategy in clinical practice, but the clinical implementation efficiency is low. The traditional method is prone to anchorage loss and reciprocating of molars. Therefore, domestic and foreign scholars continue to explore new treatment approaches and use finite element analysis to study the mechanical mechanism of distalization. 
    OBJECTIVE: To establish a three-dimensional finite element model of the maxillary complex and orthodontic appliance, and compare the initial displacement of teeth and the stress changes of periodontal ligament in two distalization methods of maxillary dentition. 
    METHODS: One adult volunteer with normal occlusion was selected, and maxillofacial cone beam CT images were taken. Based on this, a three-dimensional finite element model of maxillary bone-upper dentition-periodontal ligament-archwire-bracket-traction hook-micro-implant anchorage nail was established. Based on this model, two groups of loading modes were set, namely group A (overall distalization 2 N group) and group B (step-by-step distalization 2 N group with push spring). In each group, the traction hook height was set to 3, 5, and 7 mm, respectively, that is, A1, A2, A3, B1, B2, and B3, a total of 6 working conditions. The models in group A (A1, A2, and A3) simulated the overall distalization of the anchor pin, and the models in group B (B1, B2, and B3) simulated the step-by-step distalization of the anchor pin and the push spring. The loading force of the neck of the micro-implant anchor pin in groups A and B was 2 N. The initial displacement changes of the teeth in the horizontal, sagittal, and vertical directions and the stress distribution of the periodontal membrane were analyzed and calculated using finite element software.  
    RESULTS AND CONCLUSION: (1) Both loading methods achieved the distalization of the maxillary dentition, but the movement amount and efficiency were different. Under the same traction hook height, the loading method of the push spring step-by-step distalization in group B achieved a greater distalization of the molars compared with the loading method of the overall distalization in group A. During the overall distalization, the molars moved distally with an inclination, and the displacement was the largest when the traction hook height was 5 mm; during the step-by-step distalization of the push spring, the molars showed an overall distalization trend, and the displacement was the largest when the traction hook height was 7 mm, but the incisors had a labial inclination trend. Under the same traction hook height, the distal displacement of the molars in group B was higher than that in group A, and the equivalent stress value of the periodontal ligament paradigm in group B2 was the largest, which was 31 kPa. (2) When the micro-implant support was used to assist the overall distal displacement of the maxillary dentition, the distal displacement efficiency was highest at a traction hook height of 5 mm; when the micro-implant support-push spring was used to step-by-step distal displacement of the maxillary dentition, the distal displacement efficiency was highest at a traction hook height of 7 mm; at different traction hook heights, the constructed buccal micro-implant-push spring-traction hook system was more efficient than the overall distal displacement method, and the molars tended to be distalized as a whole. 

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    Finite element simulation analysis of personalized titanium plate design for median mandibular fracture
    Liu Lu, Wang Qi, Wang Huaisheng, Cheng Yanan, Zhuang Yan, Chen He, Wang Xinyu
    2026, 30 (14):  3568-3575.  doi: 10.12307/2026.080
    Abstract ( 9 )   PDF (2765KB) ( 0 )   Save
    BACKGROUND: Customized titanium plates are used to heal maxillofacial fractures, reducing surgical time and improving patient satisfaction. However, there is still a lack of systematic research on the optimal shape design of personalized titanium plates and their effects on fracture healing.
    OBJECTIVE: To optimize the design of personalized titanium plates for median mandibular fractures using finite element analysis.
    METHODS: The initial titanium plate and jaw model were imported into the software for static analysis, and topological optimization was performed based on the results of static analysis. The basic shape of the personalized titanium plate was designed based on the topological optimization results. Finite element analysis was performed on the personalized titanium plate, parallel double-plate titanium plate, and vertical double-plate titanium plate under three working conditions: bilateral incisor occlusion (working condition 1), right molar occlusion (working condition 2), and right incisor occlusion (working condition 3), respectively, to select the titanium plate with the best mechanical properties in the fixation of median mandibular fractures. According to the analysis results, the personalized titanium plate was further optimized and designed into three forms: straight, upward curved, and downward curved. The optimal form was selected through finite element analysis. Based on the 1 mm thick upward curved personalized titanium plate, the titanium plates with beams (cross beams and inclined beams) of 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, and 2.0 mm width were designed. The appropriate beam width was determined by finite element analysis to complete the optimization design of the personalized titanium plate.
    RESULTS AND CONCLUSION: (1) According to the topology optimization results, an “8” shaped individual titanium plate was designed. The finite element analysis showed that in working condition 1, the maximum equivalent stress of the titanium plate in the parallel double-plate titanium plate group was the smallest, and the maximum displacement of the bone end in the vertical double-plate titanium plate group was the smallest. In working conditions 2 and 3, the maximum equivalent stress of the titanium plate and the maximum displacement of the bone end in the personalized titanium plate group were the smallest, and the mechanical properties were better. (2) Under the three working conditions, the maximum equivalent stress of the titanium plate and the maximum displacement of the bone end of the linear personalized titanium plate group, the upward curved personalized titanium plate group, and the downward curved personalized titanium plate group were slightly different, but the stress distribution of the titanium plate in the upward curved personalized titanium plate group was more uniform. (3) For the 1 mm thick upward curved personalized titanium plate, when the beam width was ≥ 1.6 mm, the maximum equivalent stress of the titanium plate and the maximum displacement of the bone end were relatively stable. When the beam width was < 1 mm, the maximum displacement of the bone end exceeded the range of initial fracture healing, which was not conducive to healing. (4) In the finite element analysis of mandibular median fracture, the “8”-shaped personalized titanium plate has the advantages of more uniform stress distribution and more stable fixation than the plate shaped like a straight line (“—”). Finally, it is determined that the 1 mm beam width and 1 mm thick upward curved personalized titanium plate is the optimal design scheme for the personalized titanium plate of mandibular median fracture.
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    Protective effect of mesoporous ZLN005@polydopamine nanoparticles on chondrocytes in osteoarthritis
    Wu Tianyi, Miao Yiming, Wan Kaichen, Teng Yun, Zou Jun
    2026, 30 (14):  3576-3585.  doi: 10.12307/2026.078
    Abstract ( 26 )   PDF (2397KB) ( 9 )   Save
    BACKGROUND: Existing studies generally believe that mitochondrial dysfunction and matrix metabolism imbalance are key factors leading to the progression of osteoarthritis. ZLN005 is a peroxisome proliferator-activated receptor γ coactivator 1α agonist that can maintain mitochondrial homeostasis in an inflammatory environment.
    OBJECTIVE: To explore the protective effect of mesoporous ZLN005@polydopamine nanoparticles on osteoarthritis chondrocytes. 
    METHODS: (1) The effects of 5, 10, and 20 μmol/L ZLN005 on the proliferation of passage 4 mouse chondrocytes were observed with or without the addition of interleukin-1β. The effects of 10 and 20 μmol/L ZLN005 on the mRNA and protein expressions of aggrecan, type II collagen, SOX9, matrix metalloproteinase 13, matrix metalloproteinase 3, and thrombospondin integrin metallopeptidase 5 in passage 4 mouse chondrocytes were observed with the addition of interleukin-1β. (2) Mesoporous ZLN005@polydopamine nanoparticles were prepared, and the microscopic morphology, hydration particle size, polydispersity index and in vitro drug release of the nanoparticles were characterized. Mesoporous ZLN005@polydopamine nanoparticles of different mass concentrations were co-cultured with passage 4 mouse chondrocytes, and cell proliferation was detected by CCK-8 assay and live-dead staining. Under the condition of adding interleukin-1β, the effects of mesoporous polydopamine nanoparticles, ZLN005, and mesoporous ZLN005@polydopamine nanoparticles on the expression of aggrecan, type II collagen, SOX9, matrix metalloproteinase 13, matrix metalloproteinase 3, and thrombospondin integrin metallopeptidase 5 proteins in passage 4 mouse chondrocytes were observed. 
    RESULTS AND CONCLUSION: (1) With or without the addition of interleukin-1β, different concentrations of ZLN005 did not affect the proliferation of mouse chondrocytes. When interleukin-1β was added, 10 and 20 μmol/L ZLN005 could increase the expression of aggrecan, type II collagen, and SOX9, and reduce the expression of matrix metalloproteinase 13 and matrix metalloproteinase 3, and the effect of 20 μmol/L ZLN005 was more significant. (2) Mesoporous ZLN005@polydopamine nanoparticles showed a uniform spherical structure with an average diameter of about 350 nm, with obvious mesoporous structure and a polydispersity index of 0.09. ZLN005 could be slowly and continuously released in vitro. 5-100 μg/mL mesoporous ZLN005@polydopamine nanoparticles did not affect the proliferation of mouse chondrocytes. Under the condition of adding interleukin-1β, ZLN005 and mesoporous ZLN005@polydopamine nanoparticles could increase the expression of aggrecan, type II collagen, and SOX9 protein, and reduce the expression of matrix metalloproteinase 13, matrix metalloproteinase 3, and thrombospondin integrin metallopeptidase 5 protein, and the effect of mesoporous ZLN005@polydopamine nanoparticles was more obvious. The results show that mesoporous ZLN005@polydopamine nanoparticles can regulate the matrix metabolism of osteoarthritis chondrocytes and play a protective role. 
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    Nanofibers with gradient deposition of endothelial cell derivatives modulate behavior of Schwann cells
    Yao Lijie, Yan Yuying, Chen Siyu, Wang Yuanfei, Wu Tong
    2026, 30 (14):  3586-3596.  doi: 10.12307/2026.302
    Abstract ( 18 )   PDF (2208KB) ( 0 )   Save
    BACKGROUND: Schwann cells play a pivotal role in the process of peripheral nerve repair. By migrating to form cell bridges and secreting neurotrophic factors, Schwann cells provide essential nutrients and topographical cues for axon extension. The high specific surface area of nanofibers is conducive to the release of drugs and bioactive substances, and provides sufficient space for cell adhesion, spreading and proliferation, which is of great significance for the rapid repair of nerve tissue.
    OBJECTIVE: To explore the regulation of Schwann cell behavior by polycaprolactone aligned nanofibers deposited endothelial cell derivative microparticles.
    METHODS: Functionalized endothelial cell derivatives were extracted from the conditioned medium of human umbilical vein endothelial cells by low-temperature freeze-drying technology. Polycaprolactone aligned nanofibers were prepared by electrospinning technology. Endothelial cell derivatives were deposited on the surface of polycaprolactone aligned nanofibers in the form of microparticles by electrospraying technology, and the deposition time was 10, 20, and 30 minutes, respectively. Human umbilical vein endothelial cells (or Schwann cells) were inoculated on polycaprolactone oriented nanofibers deposited with endothelial cell derivatives, and the optimal deposition time of endothelial cell derivatives was determined by cell proliferation and growth for subsequent experiments. During the electrostatic spraying process, the glass slide was divided into five areas of equal size and a shield was covered on the glass slide. By changing the area covered by the shield (i.e., gradually decreasing from 4 areas to 0 areas), eposition time of microparticles in different regions was regulated to obtain polycaprolactone aligned nanofibers with unidirectional linear gradient deposition of endothelial cell derivative microparticles. Schwann cells were inoculated on polycaprolactone aligned nanofibers (polycaprolactone group), polycaprolactone aligned nanofibers with uniform deposition of endothelial cell derivative microparticles (uniform deposition group), and polycaprolactone aligned nanofibers with unidirectional linear gradient deposition of endothelial cell derivative microparticles (gradient deposition group), and the cell activity, morphology, and migration ability were detected.
    RESULTS AND CONCLUSION: (1) The optimal deposition time of endothelial cell derivatives was determined to be 10 minutes based on cell proliferation and growth. Scanning electron microscopy showed that endothelial cell derivatives were uniformly deposited on the polycaprolactone aligned nanofiber membrane and had superhydrophilicity. (2) CCK-8 assay results showed that the Schwann cell viability in the uniform deposition group and gradient deposition group was higher than that in the polycaprolactone group. The results of fluorescence microscopy showed that Schwann cells in the polycaprolactone group, uniform deposition group, and gradient deposition group grew along the direction of nanofibers and were in good growth state. The results of immunofluorescence staining showed that compared with the polycaprolactone group, Schwann cells in the uniform deposition group and gradient deposition group showed stronger migration ability, and the migration distance of Schwann cells in the gradient deposition group was the largest. These results indicate that unidirectional linear gradient deposition of endothelial cell derivative microparticles polycaprolactone aligned nanofibers can promote the proliferation and directional migration of Schwann cells.
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    Hard template construction of nano-beta-tricalcium phosphate and nano-hydroxyapatite root canal sealing materials
    Li Qian, Qumanguli · Abudukelimu, Shao Ziyu, Hu Yang
    2026, 30 (14):  3597-3608.  doi: 10.12307/2026.657
    Abstract ( 10 )   PDF (5158KB) ( 0 )   Save
    BACKGROUND: At present, root canal sealants have the shortcomings of poor biocompatibility, mechanical properties, and degradation, and nano-β-tricalcium phosphate and nano-hydroxyapatite materials have good biocompatibility, degradability, and sealability, and have important research value in the field of root canal sealants.
    OBJECTIVE: To construct new root canal sealing material with microscopic morphology and three-dimensional structure similar to the hard tissue structure of the tooth by controlling the nucleation, growth, and reconstruction of nano-β-tricalcium phosphate and nano-hydroxyapatite crystals by the dentin sheet hard template method
    METHODS: Totally 30 discarded third molars extracted due to impacted wisdom teeth were collected and dentin slices were prepared. Different masses of nano-β-tricalcium phosphate (or nano-hydroxyapatite) were dissolved in distilled water, and the solution was poured into a culture dish containing a dentin slice and placed in a 37°C constant temperature incubator for 12 hours to obtain 10%, 20%, and 30% nano-β-tricalcium phosphate/dentin slices and 10%, 20%, and 30% nano-hydroxyapatite/dentin slices, with pure nano-β-tricalcium phosphate and nano-hydroxyapatite as controls. The eight groups of samples were characterized and analyzed by scanning electron microscopy, Raman spectroscopy, X-ray diffractometer, and X-ray photoelectron spectroscopy.
    RESULTS AND CONCLUSION: (1) Scanning electron microscopy: Under the regulation of dentin slices, the microstructure and morphology of nano-β-tricalcium phosphate and nano-hydroxyapatite changed. The morphology of nano-β-tricalcium phosphate particles changed from irregular polygons to short rods and spheres, arranged in three-dimensional clusters, and the morphology of nano-hydroxyapatite particles changed from rods and needles to short rods and other forms. Nano-β-tricalcium phosphate and nano-hydroxyapatite particles were orderly arranged between the dentin collagen fiber network, and with the increase of nano-β-tricalcium phosphate or nano-hydroxyapatite concentration, the adhesion and agglomeration phenomenon became more and more obvious, and the arrangement became more and more dense. (2) Raman spectroscopy: Under the regulation of dentin slices, the functional groups of nano-β-tricalcium phosphate and nano-hydroxyapatite did not change, but with the increase of nano-β-tricalcium phosphate or nano-hydroxyapatite concentration, the intensity of the functional group characteristic peak increased. (3) X-ray diffraction: Under the regulation of dentin slices, the crystallinity of nano-β-tricalcium phosphate and nano-hydroxyapatite increased, the crystal structure became more complete, and the grains became smaller. Among them, 30% nano-β-tricalcium phosphate/dentin slices and 30% nano-hydroxyapatite/dentin slices had the best crystallinity. (4) X-ray photoelectron spectroscopy: Under the regulation of dentin slices, the elemental composition of nano-β-tricalcium phosphate and nano-hydroxyapatite remained unchanged, but some chemical state changes and characteristic peak intensity enhancement characteristics appeared. (5) The results show that the characterization performance of nano-β-tricalcium phosphate or nano-hydroxyapatite composites constructed with dentin slices as hard templates is stable, and dentin slices have a certain regulatory function on nano-β-tricalcium phosphate and nano-hydroxyapatite. Among them, 30% nano-β-tricalcium phosphate/dentin slices and 30% nano-hydroxyapatite/dentin slices have better characterization performance.
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    Deferoxamine-loaded strontium alginate hydrogel promotes the repair of skull injury in rats
    Wang Hao, He Qin, Wang Pingxi, Zhang Jun, Wu Zhilin
    2026, 30 (14):  3609-3617.  doi: 10.12307/2026.031
    Abstract ( 8 )   PDF (3260KB) ( 0 )   Save
    BACKGROUND: In recent years, alginate hydrogels have been widely used in bone tissue engineering to promote bone regeneration. Studies have shown that deferoxamine can promote angiogenesis and bone regeneration.
    OBJECTIVE: To construct strontium alginate/deferoxamine hydrogels and analyze their ability to promote angiogenesis and bone regeneration. 
    METHODS: (1) Strontium alginate hydrogels and strontium alginate/deferoxamine hydrogels were prepared by chemical cross-linking method, and the microscopic morphology of the hydrogels was observed by scanning electron microscopy. Strontium alginate hydrogels and strontium alginate/deferoxamine hydrogels were co-cultured with rat bone marrow mesenchymal stem cells. The cytotoxicity of the hydrogels was evaluated by live-dead cell staining and CCK-8 assay. The two hydrogels were cultured with rat bone marrow mesenchymal stem cells for osteogenic induction. The osteogenic differentiation was analyzed by alkaline phosphatase staining, alizarin red staining, and osteopontin immunofluorescence staining. The two hydrogel extracts were co-cultured with human umbilical vein cells, and the angiogenesis was analyzed by tubule formation experiment and CD31 immunofluorescence staining. (2) A 5-mm circular bone defect was made in the skull of 18 SD rats. The rats were randomly divided into three groups, with 6 rats in each group. The control group did not receive any intervention. The other two groups were injected with strontium alginate hydrogel and strontium alginate/deferoxamine hydrogel, respectively. Eight weeks after surgery, the bone repair was analyzed by Micro-CT detection and hematoxylin-eosin and Masson staining.
    RESULTS AND CONCLUSION: (1) Scanning electron microscopy showed that both hydrogels had a loose and porous structure. Live and dead cell staining and CCK-8 assay showed that the two hydrogels had no cytotoxicity. Alkaline phosphatase staining, alizarin red staining, and osteopontin immunofluorescence staining analysis showed that both hydrogels could promote the osteogenic differentiation of bone marrow mesenchymal stem cells. Tubule formation experiment and CD31 immunofluorescence staining analysis showed that compared with strontium alginate hydrogel, strontium alginate/deferoxamine hydrogel could promote angiogenesis. (2) Micro-CT detection of rat skull defect experiment showed that the new bone formation in the strontium alginate/deferoxamine group was significantly higher than that in the control group and the strontium alginate group. Hematoxylin-eosin and Masson staining showed that the new bone formation and collagen deposition in the strontium alginate/deferoxamine group were significantly better than those in the control group and the strontium alginate group. (3) The results confirm that the strontium alginate/deferoxamine hydrogel has good ability to promote angiogenesis and bone regeneration.
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    Preparation and characterization of 3D printed microstructured silk fibroin scaffold for liver injury repair
    Shi Xiaonan, Wu Xuan, Zhang Daxu, Hu Jingjing, Zheng Yazhe, Liu Yutong, Zhao Shuo, Li Weilong, Ye Shujun, Wang Jingyi, Yan Li
    2026, 30 (14):  3618-3625.  doi: 10.12307/2026.048
    Abstract ( 5 )   PDF (2086KB) ( 0 )   Save
    BACKGROUND: Liver tissue engineering is an important strategy for treating liver injury. Previous studies have achieved good results in repairing mouse liver injury using adipose derived stem cells combined with plant fiber scaffolds, electrospun silk protein scaffolds, and decellularized liver scaffolds. However, the scaffold materials have poor mechanical properties, irregular microstructures, and cannot simulate the liver microstructure to the greatest extent.
    OBJECTIVE: To fabricate microstructured silk fibroin scaffolds using 3D printing technology and evaluate their biocompatibility.
    METHODS: (1) The silk fibroin fiber obtained after degummed silkworm cocoon was dissolved in lithium bromide solution, and then glycidyl methacrylate was added for grafting. The mixed solution was dialyzed, lyophilized, and added with distilled water to prepare 3D printing bio-ink. The microstructure and 
    compressive elastic modulus of the scaffold were detected. (2) The third generation of mouse adipose derived stem cells were divided into two groups: the control group was cultured in flat plane, and the experimental group was seeded in 3D printed microstructured silk fibroin scaffold. The cytotoxicity of the scaffold was detected by CCK-8 assay. Cell proliferation was detected by live/dead staining. Cell adhesion was observed by scanning electron microscopy. After induction, the secretion of albumin and alpha-fetoprotein in the supernatant of the induced cells was detected by ELISA, and the expression of albumin, cytokeratin 18 and cytochrome P450 mRNA in the induced cells was detected by RT-PCR. (3) Nine BALB/c mice were selected, and the 3D printed microstructured silk fibroin scaffold was sutured and fixed to the surface of the liver after exposing the left lobe of the liver. The degradation of the scaffold and the tissue morphology of the liver-scaffold contact site were observed on days 2, 7, and 28 after surgery.
    RESULTS AND CONCLUSION: (1) The 3D printed microstructured silk fibroin scaffold was porous under scanning electron microscopy, with regular and uniform pore structure, pore size of 100 μm, and the compressive elastic modulus of the scaffold was 11.96 kPa. (2) 3D printed microstructured silk fibroin scaffolds were non-cytotoxic, and adipose stem cells could adhere to the scaffolds and proliferate, and were evenly distributed in the scaffolds. After hepatogenic induction culture, compared with the control group, the secretion of albumin and alpha-fetoprotein in the scaffold group increased (P < 0.05), and the expression of albumin, cytokeratin 18, and cytochrome P450 mRNA increased (P < 0.05). (3) With the extension of postoperative time, the scaffold gradually adhered to the liver surface and the scaffold gradually degraded. Hematoxylin-eosin staining showed that on day 2 after surgery, there was a clear junction between the scaffold and the liver surface, and there were more inflammatory cell infiltrations at the junction, and cells began to enter the inside of the scaffold. On day 7 after surgery, the junction between the scaffold and the liver surface began to fuse, the inflammatory cell infiltration at the junction decreased, and more cavities and more cells were formed in the scaffold. On day 28 after surgery, there was no obvious junction between the scaffold and the liver surface, the inflammatory reaction at the junction basically disappeared, and the scaffold was significantly degraded. (4) The results showed that the 3D printed microstructured silk fibroin scaffold had good biocompatibility and could promote the adhesion, proliferation, and hepatocyte-like cell differentiation of adipose-derived stem cells.
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    Repair of rat endometrial injury by using miR-424-5p modified exosome/Poloxam 407 hydrogel
    Kong Xiaojuan, Tan Zhenyu, Lei Lei
    2026, 30 (14):  3626-3635.  doi: 10.12307/2026.046
    Abstract ( 7 )   PDF (2086KB) ( 0 )   Save
    BACKGROUND: Studies have shown that treatment with exosomes and miR-424-5p mimics can promote angiogenesis and ameliorate endometrial injury. However, it is difficult for exosomes to attach to the endometrial wall after intrauterine administration, which shortens the retention time of exosomes and results in the inability of exosomes to fully exert their biological effects. Therefore, in recent years, more and more biological scaffolds have been used to deliver exosomes, so that exosomes can continue to play biological effects at the injured site.
    OBJECTIVE: To observe the effect of miR-424-5p modified exosome/Poloxam 407 hydrogel on the proliferation, migration, and angiogenesis of human umbilical vein endothelial cells, as well as the therapeutic effect on endometrial injury in rats. 
    METHODS: miR-424-5p was transfected into rat bone marrow mesenchymal stem cells by lentiviral transfection, and exosomes were subsequently extracted from bone marrow mesenchymal stem cells and identified. The expression of miR-424-5p in exosomes not transfected with lentivirus, exosomes transfected with lentivirus, and exosomes transfected with empty vector were detected by RT-qPCR to verify the transfection success. Poloxam 407 hydrogel, exosome/Poloxam 407 hydrogel, and miR-424-5p modified exosome/Poloxam 407 hydrogel were prepared respectively, and cell proliferation, migration, and capillary network formation were detected after co-culture with human umbilical vein endothelial cells. A total of 35 SD rats were randomly divided into sham operation group, model group, hydrogel group, non-transfected exosome hydrogel group, and transfected exosome hydrogel group, with 7 rats in each group. Except the sham operation group, endometrial injury models were established in the other four groups and corresponding hydrogel treatment was given, while no treatment was given in the model group. At 14 days after operation, samples were collected. The morphologic structure, angiogenesis, and receptivity indexes of endometrium were detected.
    RESULTS AND CONCLUSION: (1) In vitro cell experiment results showed that both exosome/Poloxam 407 hydrogel and miR-424-5P-modified exosome/Poloxam 407 hydrogel could promote the proliferation, migration, and capillary network formation of human umbilical vein endothelial cells, and the promotion effect of miR-424-5p-modified exosome/Poloxam 407 hydrogel was more significant. (2) Animal experiments showed that exosome/Poloxam 407 hydrogel and miR-424-5p modified exosome/Poloxam 407 hydrogel could increase the thickness and number of glands of the injured endometrial, inhibit fibrosis, promote the formation of blood vessels, and the expression of tolerance indicators, and have a good therapeutic effect on endometrial injury. Moreover, miR-424-5p modified exosome/Poloxa 407 hydrogel had a more significant therapeutic effect. These findings conclude that miR-424-5p modified exosome/Poloxam 407 hydrogel can promote tissue regeneration, angiogenesis and improve receptivity of injured endometrium in rats by promoting the proliferation, migration, and angiogenesis of human umbilical vein endothelial cells, thus playing a potential therapeutic role. 
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    3D printed methacrylated gelatin/chitosan scaffolds: evaluation of antibacterial, mechanical properties and cytocompatibility
    Li Liang, Yang Han, Suo Hairui, Guan Lu, Wang Zhenlin
    2026, 30 (14):  3636-3642.  doi: 10.12307/2025.586
    Abstract ( 5 )   PDF (1951KB) ( 0 )   Save
    BACKGROUND: 3D printing is an important means to manufacture tissue engineering scaffolds, but how to improve the antibacterial property of scaffolds is an important link that is easily overlooked in current research.
    OBJECTIVE: To construct methacrylated gelatin/chitosan antibacterial tissue engineering scaffolds by 3D printing and evaluate the antibacterial properties, mechanical properties, and cell compatibility of the scaffolds.
    METHODS: Methacrylated gelatin (3%-15%) and chitosan (0%-5%) were mixed to prepare methacrylated gelatin/chitosan composite bio-inks. The 3D printability of the bio-inks was tested at room temperature, and the appropriate bio-inks were selected for subsequent experiments. 10% methacrylated gelatin was mixed with chitosan of different concentrations (0%, 1%, 3%, and 5%) as bio-inks, and methacrylated gelatin/chitosan scaffolds were 3D printed, which were recorded as G10, G10C1, G10C3, and G10C5, respectively. The effects of the four kinds of bio-inks and four kinds of scaffolds on the growth of Escherichia coli were detected, and the compressive strength and compression modulus of the four kinds of scaffolds were detected. Mouse embryonic fibroblasts NIH3T3 were inoculated on the surface of four scaffolds. The live-dead staining was performed after culturing for 3 days to observe cell viability.
    RESULTS AND CONCLUSION: (1) The printing range of pure methacrylated gelatin was between 7% and 13%. The addition of chitosan expanded the printing range of methacrylated gelatin to a lower concentration, which could be as low as 4%. The addition of chitosan to high-concentration methacrylated gelatin increased its viscosity and made it difficult to extrude. Finally, methacrylated gelatin with a concentration of 10% was mixed with 0%, 1%, 3%, and 5% chitosan as bio-ink. (2) With the increase of chitosan concentration, the antibacterial properties of bio-ink and 3D printed scaffolds were enhanced; the compressive strength and compression modulus of 3D printed scaffolds were increased, and the viability of NIH3T3 cells on 3D printed scaffolds increased first and then decreased, among which the cell viability on G10C3 scaffold was the best. (3) The results show that by adjusting the concentration of chitosan and combining 3D printing technology, methacrylated gelatin/chitosan tissue engineering scaffolds with excellent antibacterial properties, mechanical properties and cell compatibility can be obtained.
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    Gelatin methacryloyl hydrogel loaded with injectable platelet-rich fibrin promotes skin wound repair
    Wei Dongdong, Shi Meikun, Wang Lei
    2026, 30 (14):  3643-3651.  doi: 10.12307/2025.588
    Abstract ( 7 )   PDF (2920KB) ( 0 )   Save
    BACKGROUND: Currently, gelatin methacryloyl and its composite materials are widely used in the field of skin wound repair, but single-function dressings only provide some passive protection and lack functions such as killing bacteria or regulating endogenous factors to promote skin wound healing. Injectable platelet-rich fibrin contains high concentrations of cytokines, which can promote skin wound healing. 
    OBJECTIVE: To observe the effect of gelatin methacryloyl hydrogel loaded with injectable platelet-rich fibrin on skin wound healing. 
    METHODS: (1) Injectable platelet-rich fibrin was isolated and extracted from rat venous blood. Gelatin methacryloyl/injectable platelet-rich fibrin hydrogel (denoted as GelMA/i-PRF hydrogel) was prepared by mixing equal volumes of gelatin methacryloyl and injectable platelet-rich fibrin. At the same time, pure gelatin methacryloyl hydrogel (denoted as GelMA hydrogel) was prepared. The morphology, swelling and degradation properties of the hydrogel were characterized. Staphylococcus aureus (or Escherichia coli) were inoculated on the surface of GelMA hydrogel and GelMA/i-PRF hydrogel to detect the antibacterial properties of the hydrogel. L929 cells were inoculated on the surface of the two hydrogels. The cell proliferation, viability, and migration ability were detected by CCK-8 assay, live/dead cell staining, and scratch test. (2) A full-thickness skin defect with a diameter of 10 mm was made on the back of 9 SD rats. They were randomly divided into three groups for intervention. The control group was injected with PBS at the defect site, and the other two groups were injected with GelMA hydrogel and GelMA/i-PRF hydrogel at the defect site, respectively. The wound healing was observed within 11 days after surgery. The samples were taken for hematoxylin-eosin and Sirius red staining 11 days later.
    RESULTS AND CONCLUSION: (1) GelMA/i-PRF hydrogel had a good pore structure with an average pore size of (111.4±10.4) µm. The surface roughness and swelling rate were greater than those of GelMA hydrogel, and the degradation performance was not significantly different from that of GelMA hydrogel. Compared with GelMA/hydrogel, GelMA/i-PRF hydrogel had stronger antibacterial ability, could promote L929 cell proliferation, and improve cell viability and migration ability. (2) Compared with GelMA/hydrogel, GelMA/i-PRF hydrogel can promote the healing of rat skin wounds and improve the quality of skin wound healing. (3) Gelatin methacryloyl/injectable platelet-rich fibrin hydrogel can accelerate the healing of skin wounds by promoting the proliferation and migration of skin fibroblasts, and improve the quality of skin wound healing.
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    Conductive hydrogel with cell-free fat extract repairs spinal cord injuries in rats
    Yang Biao, , Wu Zhonghuan, , Jiang Fugui, , He Chenglong, , Li Tingdong,
    2026, 30 (14):  3652-3662.  doi: 10.12307/2026.077
    Abstract ( 6 )   PDF (3096KB) ( 0 )   Save
    BACKGROUND: Conductive hydrogels can help endogenous nerve regeneration in the spinal cord, inhibit the formation of glial fibrosis and establish a neural bridge network, and promote the recovery of motor function in rats with hemisection injury model, but their ability to reduce inflammatory response after spinal cord injury is limited. Cell-free fat extract has immunomodulatory and tissue regeneration promoting effects. 
    OBJECTIVE: To explore the role of supramolecular conductive hydrogel loaded with cell-free fat extract in the repair of spinal cord injury in rats.
    METHODS: (1) Cell-free fat extract was extracted from the inguinal adipose tissue of SD rats. Agar/gelatin hydrogel and agar/gelatin/polypyrrole hydrogel were prepared respectively, and the micromorphology, rheology, swelling rate, and conductivity of the hydrogel were characterized. Agar/gelatin/polypyrrole hydrogel loaded with cell-free fat extract was prepared, and the in vitro drug release performance of the hydrogel was tested. (2) A total of 80 SD rats were used to establish a hemisection model of spinal cord injury and randomly divided into four intervention groups: the model group (n=20) received no intervention; the ordinary hydrogel group (n=20), the conductive hydrogel group (n=20), and the drug-loaded conductive hydrogel group (n=20) were implanted with agar/gelatin hydrogel, agar/gelatin/polypyrrole hydrogel, and agar/gelatin/polypyrrole hydrogel loaded with cell-free fat extract at the injured spinal cord, respectively. On day 42 after surgery, the motor function of the right hind limb of the rats was evaluated by BBB score and inclined board test. The morphology of the spinal cord injury tissue of the rats was observed by hematoxylin-eosin staining. The expression levels of CD86, CD206, microtubule-associated protein 2, tubulin βIII, chondroitin sulfate 56, neurofilament protein 200, and myelin basic protein in the spinal cord injury center were detected by immunofluorescence. The degree of axonal myelination was evaluated by fast blue staining and transmission electron microscopy. On day 14 after surgery, the levels of tumor necrosis factor α, interleukin 1β, interleukin 10, and interleukin 6 in the injured spinal cord tissue were detected by ELISA.
    RESULTS AND CONCLUSION: (1) Agar/gelatin hydrogel and agar/gelatin/polypyrrole hydrogel presented interconnected porous structures with high porosity and rheological properties. The pore size and swelling rate of agar/gelatin/polypyrrole hydrogel were lower than those of agar/gelatin hydrogel (P < 0.05), and the conductivity was higher than that of agar/gelatin hydrogel (P < 0.05). After soaking in PBS, the cell-free fat extract showed slow and sustained release from the agar/gelatin/polypyrrole hydrogel, with a cumulative release rate of about 31% in 5 days. (2) Compared with the other three groups, the motor function of the hind limbs of rats in the drug-loaded conductive hydrogel group was significantly improved, the area of the cyst in the spinal cord injury area was significantly reduced, the levels of tumor necrosis factor α, interleukin 1β, and interleukin 6 in the spinal cord tissue decreased, and the level of interleukin 10 increased. The expression of CD86 and chondroitin sulfate 56 was significantly reduced, and the expression of CD206, microtubule-associated protein 2, tubulin βIII, neurofilament protein 200, and myelin basic protein was significantly increased, and myelin regeneration was obvious. The results showed that the drug-loaded conductive hydrogel reduced the activity of microglia/macrophages and the expression of proinflammatory factors in the injured spinal cord tissue of rats, promoted the differentiation of endogenous neural stem cells into neurons, and ultimately promoted axon regeneration, myelination of regenerated axons, and recovery of neural function.
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    Natural porous biosilica loaded with mupirocin for treatment of infectious wounds
    Zhang Chunlong, Peng Baowei, Chang Jiang, Yang Chen, Xu Yuhong
    2026, 30 (14):  3663-3674.  doi: 10.12307/2026.107
    Abstract ( 4 )   PDF (3669KB) ( 0 )   Save
    BACKGROUND: Synthetic mesoporous silica can be used as a drug delivery carrier for wound treatment, but the synthesis process of this carrier is relatively complex and requires the use of toxic chemicals.

    OBJECTIVE: To extract natural porous silica from diatoms to achieve efficient loading of the antibiotic mupirocin for the treatment of infected wounds. 

    METHODS: (1) Diatom frustules (natural porous silica) were extracted from Nitzschia, Stratus algae and Amphiprion by high-temperature sintering. The morphology, size, pore structure and biocompatibility of the three diatom frustules were characterized. Synthetic mesoporous silica and the three diatom frustules were immersed in mupirocin solution to prepare drug delivery systems, and the drug loading, encapsulation efficiency and in vitro drug release behavior were tested. According to the drug loading and encapsulation efficiency, suitable diatom frustules were selected for subsequent experiments. The inhibitory effect of Stratus algae frustules on Staphylococcus aureus and Escherichia coli was detected by the supernatant containing mupirocin collected in the inhibition zone experiment and in vitro drug release behavior. (2) Twelve SD rats were selected, and two round full-thickness skin wounds with a diameter of 1.0 cm were made on the back of each rat, and Staphylococcus aureus suspension was dripped onto the wound. After 24 hours of bacterial liquid dripping, the rats were randomly divided into four intervention groups: the blank group (n=3) had no intervention on the wound; the mupirocin group (n=3) had mupirocin solution dripped onto the wound; the straight-chain algae frustule group (n=3) had straight-chain diatom frustule solution dripped onto the wound, and the drug-loaded straight-chain algae frustule group (n=3) had straight-chain diatom frustule solution loaded with mupirocin dripped onto the wound, and the wound healing was observed. At 6 and 24 hours after intervention, the antibacterial effect was detected by agar plate coating experiment. On day 9 after intervention, the wound edge tissue was histologically observed by hematoxylin-eosin staining and Masson staining.

    RESULTS AND CONCLUSION: (1) Different diatom frustules had different shell structures, showing the exquisite micro-nanostructure of natural diatom frustules. Nitzschia, Stratus, and Bryophyta frustules at concentrations of ≤25 μg/mL had no significant cytotoxicity to human umbilical vein endothelial cells and L929 cells. They showed drug loading efficiency comparable to or even better than that of synthetic mesoporous silica. The drug loading and encapsulation efficiency of Stratus frustules were higher than those of Nitzschia and Bryophyta frustules, and they could be used for subsequent drug loading. All four materials had good in vitro drug release properties, among which mupirocin had a higher cumulative release amount and release rate in Stratus frustules. Drug-loaded Stratus frustules could significantly inhibit the growth and reproduction of Staphylococcus aureus and Escherichia coli, and still showed strong antibacterial activity after one week of in vitro drug release. (2) At 24 hours after intervention, the number of colonies on the wound surface of the drug-loaded diatom frustule group was less than that of the other three groups (P < 0.05). Compared with the straight algae shells and mupirocin, the drug-loaded diatom shells accelerated the wound healing speed, and the wound healing rate exceeded 95% on day 9 after intervention. Hematoxylin-eosin staining and Masson staining showed that compared with the blank group and the straight algae shell group, the mupirocin group and the drug-loaded straight algae shell group had less inflammatory cell infiltration, shorter wound length, and more collagen deposition. The results show that diatom shells loaded with mupirocin can significantly accelerate wound healing.
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    Effects and mechanisms of natural polyphenol-based hydrogels in promoting bone repair
    Gong Yukang, Ye Gaoqi, Wang Chenhao, Chen Dejin, Gao Wenshan
    2026, 30 (14):  3675-3686.  doi: 10.12307/2026.055
    Abstract ( 6 )   PDF (2380KB) ( 0 )   Save
    BACKGROUND: In recent years, natural polyphenol-modified hydrogels have shown great application prospects in improving the immune microenvironment of bone regeneration.
    OBJECTIVE: To summarize the construction strategies of different types of natural polyphenol-modified hydrogel bone repair materials and the effects, mechanisms and repair effects of regulating the immune microenvironment of bone regeneration. 
    METHODS: PubMed, ScienceDirect, CNKI and WanFang databases were used to search the literature published from the establishment of each database to 2024. The search terms were “natural polyphenol, tannic acid, chlorogenic acid, quercetin, resveratrol, curcumin, rosmarinic acid, epigallocatechin gallate, gallic acid, proanthocyanidin, bone, hydrogel” in Chinese and English. The resulting literature was initially screened by reading to exclude duplicates and irrelevant content. Finally, 61 papers were included for review.
    RESULTS AND CONCLUSION: Natural polyphenols have bone-enhancing, antimicrobial, anti-inflammatory and antioxidant properties, which can effectively improve the immune microenvironment of bone regeneration, but solubility problems lead to their poor bioavailability. Introducing natural polyphenols into hydrogels can improve their bioavailability. Common reaction/interaction introduction modes of natural polyphenols in bone repair materials include hydrogen bonding, metal-polyphenol networks, covalent interactions (esterification, etherification, and amidation), polyphenol-borate complexation, poloxamer micelles, mesoporous particles, nanoparticles, etc. Natural polyphenols can give hydrogel materials anti-inflammatory, antioxidant, antibacterial and osteogenic properties, regulate the immune microenvironment of bone regeneration, and promote bone repair. However, due to the diversity of natural polyphenols, the mechanism of action of natural polyphenols and the selection of polyphenols in composite materials need further study.

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    Hydrogels: multifunctional effects from sports injury treatment to sports performance enhancement
    Zhang Shuai, Zhu Jiabin
    2026, 30 (14):  3687-3693.  doi: 10.12307/2026.028
    Abstract ( 4 )   PDF (934KB) ( 0 )   Save
    BACKGROUND: Hydrogel materials have shown unique application prospects in sports injury repair, sports monitoring, and flexible electronic devices due to their excellent high hydration, biocompatibility, self-healing ability, and adjustability.
    OBJECTIVE: To review the basic properties and classification of hydrogels and their current application in sports injuries. 
    METHODS: PubMed, CNKI, WanFang, and VIP databases were searched with Chinese and English search terms “hydrogel materials, athletic sports, flexible sensors, wearable devices, sports injuries, sports performance.” 
    RESULTS AND CONCLUSION: Hydrogels can be divided into physical cross-linked hydrogels and chemical cross-linked hydrogels according to the cross-linking mode, natural hydrogels and synthetic hydrogels according to the source, and traditional hydrogels and smart hydrogels according to the response characteristics. Self-healing hydrogels have good self-healing ability and can automatically repair after sports injuries, reduce secondary injuries, and improve the treatment effect. Smart hydrogels can change their properties according to external stimuli and adapt to different usage requirements, showing good application prospects in sports monitoring and auxiliary treatment. The application of hydrogels in flexible electronic devices provides a new technical path for real-time data collection and athlete health monitoring. Despite this, the current application of hydrogels in the field of sports injuries still faces some technical difficulties, such as mechanical properties, durability, and large-scale production of materials. With the continuous advancement and innovation of technology, hydrogel materials will play an increasingly important role in improving sports performance, promoting sports rehabilitation, extending the service life of equipment, and improving sports safety.

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    Different preparation methods of silk fibroin and its application in the construction of small-diameter tissue-engineered blood vessels
    Yang Lei, Liu Xinfang, Luo Sidong, Zhang Hongan, Wang Yeyang, Chen Weijian
    2026, 30 (14):  3694-3701.  doi: 10.12307/2026.106
    Abstract ( 3 )   PDF (1084KB) ( 0 )   Save
    BACKGROUND: Silk fibroin materials have the advantages of excellent mechanical properties, biocompatibility, biodegradability and low immunogenicity, and are expected to show broad application prospects in small-diameter artificial blood vessels.
    OBJECTIVE: To systematically review the application status and progress of silk fibroin in constructing small-diameter tissue engineering blood vessels.
    METHODS: The Chinese and English search terms consisted of “silk fibroin, vascular scaffold, polycaprolactone, polyurethane, collagen, polylactic acid, small diameter, vascular tissue engineering.” The search was conducted in databases such as PubMed, China National Knowledge Infrastructure (CNKI), and WanFang for articles published from 2011 to 2025. Finally, 75 articles were included in the review.
    RESULTS AND CONCLUSION: Compared with other common biomaterials, such as polycaprolactone, polylactic acid, polyurethane, and collagen, silk fibroin has obvious advantages in mechanical properties, biocompatibility, degradability and immunogenicity, and the extraction procedure of the material is simple. The ideal silk fibroin vascular scaffold can be prepared by dip coating, gas foaming, freeze-drying, electrospinning, bioprinting and multiple combination techniques. It not only has the advantages of high efficiency, easy adjustment, low cost, safety and reliability, but also has the disadvantages of insufficient stability, high viscosity and low accuracy. The small-diameter silk fibroin vascular scaffold shows good anticoagulant, endothelialization and mechanical properties. However, silk fibroin still has some difficulties to overcome before clinical application, mainly in the following aspects: automated bioprinting to produce high-precision silk fibroin vascular tissue rarely enters clinical trials; its availability is affected by the seasonality and regionality of mulberry leaves; silk fibroin is difficult to maintain stability during long-term storage.
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    Application of electrospinning in artificial dura mater substitutes: diverse biological functions
    Li He, Wang Yu, Xie Zikang, Jiang Tao
    2026, 30 (14):  3702-3708.  doi: 10.12307/2026.027
    Abstract ( 4 )   PDF (1354KB) ( 0 )   Save
    BACKGROUND: Many studies have applied electrospinning to the study of artificial dura mater substitutes, and designed multifunctional artificial dura mater, which can not only better simulate the natural dura mater structure, but also play biological functions such as promoting healing, preventing adhesion, and antibacterial. 
    OBJECTIVE: To systematically summarize the research progress of electrospinning technology in artificial dura mater substitutes. 
    METHODS: CNKI, WanFang, and PubMed databases were searched with Chinese and English search terms “electrospinning dura mater, dura mater substitute, artificial dura mater patch” for articles published from inception to September 2024. Finally, 64 articles were included for summarization by reading the titles and abstracts to select and exclude the literature with low relevance.
    RESULTS AND CONCLUSION: Artificial dura mater is mainly divided into two categories: non-degradable and degradable. It plays an important role in repairing dura mater defects and is also a hot topic in the research of biological tissue engineering. Electrospinning can combine chemistry, materials science, pharmacology and other fields to design artificial dura substitutes from the structural and functional requirements, processing a series of new artificial dura mater such as biomimetic oriented fiber type, asymmetric double layer type, and multi-layer composite type. Meanwhile, it also gives many important biological functions such as sealing, anti-adhesion, antibacterial, and promoting nerve tissue repair, which makes the artificial dura mater change from traditional simple patches to multifunctional tissue biological scaffolds. Electrospinning provides assistance for the future development and research of ideal artificial dura mater substitutes, and brings new ideas for solving the problem of dura mater injury.
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    Characteristics and strategies of 3D-printed biomimetic bioceramic scaffolds for repairing jaw defects
    Xiong Jiaying, Shen Jieyi, Lyu Jiahong
    2026, 30 (14):  3709-3716.  doi: 10.12307/2026.653
    Abstract ( 3 )   PDF (1606KB) ( 0 )   Save
    BACKGROUND: 3D-printed biomimetic bioceramic scaffolds, characterized by personalized design, superior biocompatibility, and osteogenic potential, represent an ideal solution for jaw defect repair.
    OBJECTIVE: To systematically review recent advances in 3D-printed bioceramic scaffolds with biomimetic structures for jaw repair.
    METHODS: Literature searches were conducted on PubMed and CNKI from database inception to 2025 using Chinese and English search terms “3D printing, scaffold, bionic, biomimetic, ceramic, maxillofacial repair, jaw repair.” Sixty-eight relevant articles were included for critical analysis.
    RESULTS AND CONCLUSION: Jaw defect repair requires balancing anatomical restoration and functional recovery. 3D-printed biomimetic bioceramic scaffolds, through designs such as gradient porosity and mechanical adaptation, precisely match defect morphology, outperforming traditional grafts (e.g., autologous bone) in bioactivity and osteoconductivity. Osteomimetic structures (e.g., trabecular bone simulation) and bioinspired hierarchical architectures enhance osseointegration efficiency. The current research on 3D-printed bioceramic scaffolds in jaw repair is still in its infancy. While initial studies confirm mechanical stability and biosafety, long-term degradation kinetics, immune responses, and clinical scalability require further investigation. 

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    Key points of zirconia all-ceramic restoration materials in clinical application
    Tang Yiyang, Xiao Yuxin, Sun Hening, Li Chunxiao, Zhang Yutong, Fu Jiale
    2026, 30 (14):  3717-3725.  doi: 10.12307/2026.613
    Abstract ( 3 )   PDF (1935KB) ( 0 )   Save
    BACKGROUND: Zirconia ceramics have high fracture toughness, compressive strength, wear resistance, corrosion resistance, and aesthetics similar to natural teeth, so they are increasingly used in dental restoration.
    OBJECTIVE: To summarize the key operation points of zirconia ceramics in clinical application from the aspects of tooth preparation, impression taking, try-in and adjustment, and bonding, as well as the special precautions for high permeability zirconia.
    METHODS: The pieces of literature on zirconia were searched in CNKI and PubMed databases by computer. The search terms were “zirconia, high transparency zirconia, bonding, surface treatment, tooth preparation, all-ceramic restoration” both in English and Chinese. A total of 85 articles were finally included for review.
    RESULTS AND CONCLUSION: Zirconia ceramic restoration material is an ideal choice for clinical aesthetic restoration, but its successful application depends on the comprehensive control of material properties, clinical operation, and postoperative maintenance. The amount of tooth preparation should be considered according to tooth color, occlusal force, and the flexural performance of materials to ensure that the prosthesis has excellent mechanical properties and aesthetics. Edge design has an important effect on the suitability and durability of zirconia restorations. Reasonable edge shape can reduce stress concentration and increase the service life of the restorations. Oral digital impressions reduce procedure steps and are superior to traditional impressions in accuracy. Additionally, when bonding zirconia prosthesis, it is necessary to perform proper surface treatment on abutment teeth and prosthesis based on thorough cleaning and select high-performance cement to achieve the best bonding strength and aesthetics. The application of digital technology and artificial intelligence is expected to further improve the repair effect of zirconia ceramic restoration, thus gaining wider clinical applications.

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    Role of enamel demineralization and remineralization in orthodontic treatment
    Ma Xinyu, Li Mengying, Li Huang, Han Lei
    2026, 30 (14):  3726-3735.  doi: 10.12307/2025.590
    Abstract ( 2 )   PDF (1339KB) ( 0 )   Save
    BACKGROUND: Enamel demineralization, as one of the common syndromes during orthodontic treatment, is mostly caused by patients’ poor dietary habits and neglect of oral hygiene while wearing appliances. It usually manifests as shapeless, opaque, chalky lesions on enamel surface (white spot lesions).
    OBJECTIVE: To analyze the causes of enamel demineralization, as well as current clinical research status and progress of remineralization methods for enamel demineralization during orthodontic treatment by reviewing the literature. 
    METHODS: We searched articles about enamel demineralization and remineralization in PubMed, CNKI, and WanFang databases with the keywords including “orthodontics, enamel, demineralization, remineralization, white spot lesions, early enamel caries lesions, clinical applications” in Chinese and English. Finally, 89 articles were included. 
    RESULTS AND CONCLUSION: Enamel demineralization during orthodontic treatment usually has a negative impact of aesthetics and function on the process and outcome. Though the use of remineralizing agents inhibits unfavorable effects and promotes the redeposition of calcium and phosphate ions on the enamel surface. Common commonly used laboratory methods can monitor the degree of enamel demineralization and remineralization, while longer-term efficacy still needs to be confirmed by further clinical follow-ups.
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    Application of quantitative light-induced fluorescence technology in diagnosis of oral diseases
    Du Huijing, Chen Shichun, Liu Yinuo, Yang Yutong, Wang Ruohan, Yang Xueying, Ma Zhe, Yan Rui
    2026, 30 (14):  3736-3744.  doi: 10.12307/2025.881
    Abstract ( 5 )   PDF (1464KB) ( 0 )   Save
    BACKGROUND: As an emerging optical diagnostic method, quantitative light-induced fluorescence technology has received extensive attention and application in the field of oral disease diagnosis in recent years. 
    OBJECTIVE: To review the application of quantitative light-induced fluorescence technology in the diagnosis and treatment of oral diseases.  
    METHODS: PubMed database and CNKI database were searched by computer. The Chinese search terms were “quantitative light-induced fluorescence, early caries, osteomyelitis of jaw bone, oral malodor, plaque, cracked teeth, wear teeth.” The English search terms were “quantitative light-induced fluorescence/QLF, dental caries/dental decay, osteomyelitis of jaw/maxillary osteomyelitis, oral malodor/dental bacteria, cracked tooth syndrome/enamel crack/tooth cracked/enamel fracture, tooth wear.” The quality of the retrieved Chinese and English literature was assessed. Finally, 60 articles meeting the criteria were included for review. 
    RESULTS AND CONCLUSION: (1) Quantitative light-induced fluorescence technology has shown effectiveness in the diagnosis of oral diseases such as early caries, osteomyelitis of the jaw, tooth cracks, oral malodor and tooth wear, and can provide a more accurate diagnosis than traditional methods, especially in the detection of early caries and tooth cracks. (2) Quantitative light-induced fluorescence technology can be combined with artificial intelligence, optical coherence tomography, digital photography and other technologies to improve the efficiency and accuracy of diagnosis. (3) Quantitative light-induced fluorescence technology has the advantages of multi-purpose, non-invasive, radiation-free, real-time judgment, and high sensitivity, and is a promising tool in the diagnosis of oral diseases. Despite some challenges, such as environmental condition requirements, equipment cost, and popularity, and less research support compared to other established technologies, quantitative light-induced fluorescence technology still shows great potential for application. Future research needs to further improve the accuracy and reliability of quantitative light-induced fluorescence techniques, explore combinations with other diagnostic techniques, and promote their standardization for use in broader clinical settings.  
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    Association between peri-implantitis and cytokine gene polymorphisms
    Huang Rui, Wang Jie, Zhang Ye, Liao Jian
    2026, 30 (14):  3745-3752.  doi: 10.12307/2026.614
    Abstract ( 4 )   PDF (1386KB) ( 0 )   Save
    BACKGROUND: The occurrence of peri-implantitis is not only related to exogenous factors, and genetic risk factors may contribute to the development of implant diseases. This is mainly reflected in the expression changes caused by the polymorphisms of various cytokines. Therefore, the association between peri-implantitis and the gene polymorphisms of various related cytokines has received much attention.
    OBJECTIVE: To summarize the correlation between peri-implantitis and the polymorphisms of multiple common related genes.
    METHODS: Using “peri-implantitis, peri-implant inflammation, peri-implant diseases, gene variation, polymorphism” as Chinese search terms and “peri-implantitis, genotype, SNP, single nucleotide polymorphisms, polymorphism, variant, dental implant failures, dental implant loss” as English search terms, a computer was used to search the relevant literature indexed in CNKI, Web of Science, and PubMed databases. Screening was carried out by reading the titles and abstracts, and finally 65 articles were included for review. 
    RESULTS AND CONCLUSION: The correlations between the gene polymorphisms of the interleukin family (interleukin 1, interleukin 4, interleukin 6, interleukin 10, and interleukin 17), tumor necrosis factor α, nuclear factor κB receptor activator/nuclear factor κB receptor activator ligand/osteoprotegerin system, Fcγ receptor, CD14 and the occurrence of peri-implantitis remain controversial. Regarding the relationship between matrix metalloproteinases and peri-implantitis, the results of early studies are contrary to those of recent years. The gene polymorphisms of epidermal growth factor and CXC chemokine receptor 2 have been found to be associated with the susceptibility to peri-implantitis in the Chinese population. The main reasons for the controversial correlations are the small sample size, the potential limitations of data collected from specific geographical populations, and the lack of more specific experimental evidence.

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