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    28 October 2017, Volume 21 Issue 30 Previous Issue    Next Issue
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    Kyphoplasty with polymethylmethacrylate bone cement treats Kummell disease in the elderly 
    Li Zhi-jun, Zheng Yu-peng, Jiang Tian-wei, Shi Yong-chen
    2017, 21 (30):  4757-4762.  doi: 10.3969/j.issn.2095-4344.2017.30.001
    Abstract ( 353 )   PDF (1139KB) ( 443 )   Save
    BACKGROUND: Percutaneous kyphoplasty is an effective method for the treatment of osteoporotic vertebral compression fracture, because it can relieve pain quickly, restore the vertebral height and correct kyphosis. However, little is reported on percutaneous kyphoplasty for the treatment of Kummell disease.
    OBJECTIVE: To evaluate the efficacy and safety of kyphoplasty with polymethylmethacrylate bone cement in treating Kummell disease in the elderly.
    METHODS: Thirty-one patients (6 males and 25 females) with Kummell disease, aged 58-72 years old, were included in this study. Affected segments involved T11 in 5 cases, T12 in 11 cases, L1 in 8 cases, L2 in 4 cases, L3 in 2 cases, L4 in 1 case. All the patients underwent percutaneous kyphoplasty with polymethylmethacrylate bone cement. The clinical effect was evaluated by pain, viability and imaging changes before and after therapy.
    RESULTS AND CONCLUSION: (1) Pain relief: The visual analogue scale score for all the 31 patients at 3 days, 3 months and 12 months after the surgery were significantly lowered compared to the preoperative scores (P < 0.05). (2) Viability: The mean Oswestry disability index scores for 31 patients at 3 days, 3 months and 12 months after the surgery were significantly lowered compared to the preoperative scores (P < 0.05). (3) Imaging evaluation: The anterior vertebral height and vertebral height at middle line for 31 patients at 3 days, 3 months and 12 months after the surgery were higher compared to the preoperative data (P < 0.05), and Cobb angles were significantly lowered compared to the preoperative data (P < 0.05). (4) Adverse reaction: There was no leakage of bone cement in the vertebral canal and vein, and no adverse reaction occurred related to bone cement. In summary, the kyphoplasty with polymethylmethacrylate bone cement is safe and effective in treating Kummell disease in the elderly. It can fast relieve pain, improve patients quality of life, and reconstruct the spinal stability.
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    Cement leakage factors in clinical vertebroplasty
    Chen Jia-lin, Chen Xi, Yang Jun, Wang Jun, Geng Tian-yong, An Cheng-ling, Hu Ting-ye
    2017, 21 (30):  4763-4768.  doi: 10.3969/j.issn.2095-4344.2017.30.002
    Abstract ( 518 )   PDF (1131KB) ( 382 )   Save
    BACKGROUND: Vertebroplasty (PVP) and kyphoplasty (PKP) are important methods for the treatment of osteoporotic vertebral compression fractures in the elderly. Although bone cement has certain liquidity and vertebral fractures are often in different situations, bone cement leakage rate is still high.
    OBJECTIVE: Based on the theoretical discussion and clinical analysis, to study the theoretical causes of bone cement leakage and effective prevention methods.
    METHODS: A total of 162 cases (186 vertebrae) were treated with three methods of vertebroplasty. Group A: 64 cases with 78 vertebrae were treated with conventional cemented vertebroplasty; Group B: 57 cases with 65 vertebrae were treated with cemented vertebroplasty using cement pump; Group C: 41 cases with 43 vertebrae were treated with balloon kyphoplasty. The leakage of bone cement was observed in three groups.
    RESULTS AND CONCLUSION: Of the 186 vertebrae, postoperative bone cement leakage occurred in 25 vertebrae, with the leakage rate of 13% (25/186). Group A had bone cement leakage in 11 vertebrae, and the leakage rate was 14% (11/78). Group B had bone cement leakage in 8 vertebrae, and the leakage rate was 12% (8/65). Group C had bone cement leakage in 6 vertebrae, and the leakage rate was 14% (6/43). There was no significant difference among the three groups in the leakage rate of bone cement (P > 0.05). That is to say, the causes of bone cement leakage are not completely controllable, and the leakage position has some randomness. Strict and careful imaging monitoring is an intuitive method to prevent bone cement leakage.
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    Effects of zinc-containing magnesium alloys on proliferation of human osteosarcoma U2OS cells in vitro 
    Zhao Xiao-kui, He Bao-hua, Shao Nan, Zhang Sheng-guo, Yang Wan-shi
    2017, 21 (30):  4769-4774.  doi: 10.3969/j.issn.2095-4344.2017.30.003
    Abstract ( 447 )   PDF (7199KB) ( 202 )   Save
    BACKGROUND: Alloying can improve the corrosion resistance and slow the degradation of pure magnesium. In addition, increasing studies have shown that zinc has good antitumor effect.
    OBJECTIVE: To evaluate the effect of zinc-containing magnesium alloy on the proliferation and apoptosis of human osteosarcoma U2OS cells in vitro.
    METHODS: The corrosion resistance of pure magnesium and different zinc-containing (2%, 4%, 6%) magnesium alloys were observed and compared by the hydrogen release assay in the Hank’s solution. MTT assay was used to examine the effects of different zinc-containing magnesium alloy extractions on the proliferation of U2OS cells (or MC3T3-E1 cells) after co-culture of 1, 3, 5 days. After 24-hour co-culture with pure magnesium, different zinc-containing magnesium alloys and titanium alloy extractions, the apoptotic rates of U2OS cells were analyzed by flow cytometry, and the expression of apoptosis-related proteins were detected by western blot assay.

     

    RESULTS AND CONCLUSION: The corrosion resistance of pure magnesium was improved after addition of Zn within the initial 100 hours, and the magnesium alloy containing 4% zinc exhibited the optimal corrosion resistance. Different zinc-containing magnesium alloy extractions obviously inhibited the U2OS proliferation in a zinc level-depended manner, and the cytotoxicity of different zinc-containing magnesium alloy extractions to MC3T3-E1 was graded 0-1. Different zinc-containing magnesium alloy extractions could also induce obvious apoptosis in U2OS cells in a zinc level-depended manner. Different zinc-containing magnesium alloy extractions, especially the magnesium alloy containing 4% zinc, up-regulated the expression of p53 and Bax proteins and down-regulated the expression of Bcl-2 protein in U2OS cells, leading to the disorder of Bcl-2/Bax. These findings suggest that different zinc-containing magnesium alloy extractions can inhibit the proliferation and induce apoptosis of U2OS cells in vitro.
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    Low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate in the repair of articular cartilage defects
    Hao De-feng, Zhang Lu-qing, Liu Yu-dong
    2017, 21 (30):  4775-4780.  doi: 10.3969/j.issn.2095-4344.2017.30.004
    Abstract ( 375 )   PDF (4544KB) ( 244 )   Save
    BACKGROUND: As reported, the use of low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate in rabbit articular cartilage defect repair is ideal, which can promote the healing of defect site. However, the conclusion has not been further confirmed.
    OBJECTIVE: To investigate the effect of low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate in repairing articular cartilage defects in rabbits.
    METHODS: Sixty rabbits were anesthetized using 20% urethane solution injection at a dose of 4mL/kg, and a model of articular cartilage defect (3 mm in diameter and 3 mm in depth) was made on the femoral ankle of each rabbit. Then, there were three groups in this study: model group with no intervention, sodium hyaluronate group with intra-articular injection of sodium hyaluronate, and observation group with intra-articular injection of sodium hyaluronate combined with low-intensity pulsed ultrasound. Gross observation, hematoxylin-eosin staining, safranin O staining and Wakitani scores were compared among groups after repair.
    RESULTS AND CONCLUSION: (1) At 5 weeks after repair, in the model group, the defect size was reduced with uneven surface that was lower than the surrounding tissues; in the sodium hyaluronate group, there was a clear boundary between the defect and surrounding cartilage tissues; in the observation group, the defect was completely repaired. (2) After 2 and 5 weeks, the Wakitani scores in the observation group were significantly lower than those in the other two groups (P < 0.05). (3) After 5 weeks, in the observation group, the cells around the defect site arranged regularly, and in the sodium hyaluronate group, there were a large amount of cartilage-like cells in few cartilage regions. In the model group, there were irregularly arranged capillary blood vessels after 2 and 5 weeks. (4) After 5 weeks of repair, cartilage tissues with normal structure were found in the defect site in the observation group, and deeply stained proteoglycan and cartilage lacunae were observed in the sodium hyaluronate group, while in the model group, the deep layer stained white was observed at 2 and 5 weeks after repair. To conclude, low-intensity pulsed ultrasound combined with intra-articular injection of sodium hyaluronate can achieve the ideal repair effect on rabbit articular cartilage defects.
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    Bone marrow mesenchymal stem cells/biphasic calcium phosphate ceramics for cartilage repair in juvenile rats
    Li Guo-wei, Guo Yuan-qing, Chen Tao, Zhang Kui-bo, Yu Bing, Zhang Da-wei, Zhang Rong-kai
    2017, 21 (30):  4781-4786.  doi: 10.3969/j.issn.2095-4344.2017.30.005
    Abstract ( 330 )   PDF (4609KB) ( 237 )   Save
    BACKGROUND: The pathogenesis of pediatric femoral head necrosis is associated with cartilage injury of the hip joint induced by stress and inflammation.
    OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cells (BMSCs)/biphasic calcium phosphate ceramics (B-CPC) on cartilage repair in juvenile rats.
    METHODS: Thirty male Sprague-Dawley rats, aged 1 week, were randomized into three groups. No intervention was done in blank group. A juvenile rat model of articular cartilage injury was made using improved Hulth’s method in control and observational groups, followed by implantation of BMSCs/hydroxyapatite and BMSCs/B-CPC, respectively. Four weeks later, the rat articular cartilage was observed pathologically, and MTT and flow cytometry were employed to detect chondrocyte proliferation and apoptosis, respectively.  
    RESULTS AND CONCLUSION: The articular cartilage of the rats in the blank group was smooth and complete. In the control group, articular cartilage damage was obvious, presenting with rupture, defect and irregularity of the articular cartilage surface, as well as unclear four-layer structure of the cartilage. In the observational group, articular cartilage injury was repaired to some extent. At the same observation time, the cell viability was significantly increased in the observational group compared with the control group (all P < 0.05), and the proportion of apoptotic cells was significantly decreased (all P < 0.05). To conclude, BMSCs/B-CPC composite can promote the cartilage repair in juvenile rats.
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    Injectable hyaluronic acid carrying autologous chondrocytes repairs cartilage defects
    Zhao Feng, He Wei, Liu Shao-jun, Wang Hui, Lv Ya-jun, Kang Kai, Zhang Guo-ping
    2017, 21 (30):  4787-4792.  doi: 10.3969/j.issn.2095-4344.2017.30.006
    Abstract ( 388 )   PDF (1287KB) ( 364 )   Save
    BACKGROUND: In the cartilage tissue engineering materials, hyaluronic acid as the representative of polysaccharide materials has good material-cell interface that is beneficial to the growth of chondrocytes, which has become a hot research topic in recent years.
    OBJECTIVE: To explore the feasibility of injectable hyaluronic acid material loaded with chondrocytes to repair cartilage defects in rats.
    METHODS: Ninety Sprague-Dawley rats were selected to prepare a cartilage defect model, and they were randomly divided into three groups at the 2nd day after modeling. The experimental group was injected hyaluronic acid hydrogel loaded with chondrocytes into the articular cavity, the control group was injected with hyaluronic acid hydrogel into the articular cavity, and the blank control group received no intervention. At 1, 3 and 6 weeks after injection, the repaired cartilage tissues were taken out for hematoxylin eosin staining, Masson staining, and scanning electron microscope observation, and the expression of heme oxygenase and level of collagen were detected.
    RESULTS AND CONCLUSION: (1) Hematoxylin eosin staining: at 6 weeks after injection, granulation tissues filled the repair area in the blank control group. The control group was full of yellowish white tissues in the repair area, with distinct boundary with the normal cartilage, the surface was not smooth and the lymphocytes were reduced compared with those at 3 weeks. Repair tissue of the experimental group was semi-transparent and showed a fuzzy boundary with the normal cartilage, and moreover, lymphocyte was significantly reduced compared with those at 3 weeks. (2) Masson staining: at 6 weeks after injection, collagen fiber synthesis in the repair area in the experimental group was the best, successively followed by the control group and the blank control group. (3) Scanning electron microscope observation: at 6 weeks after injection, collagen fiber arrangement in the repair area was irregular and partially broken in the blank control group, and the arrangement became more orderly in the control group but still partially broken. The collagen fibers in the experimental group were arranged orderly, and the boundary with normal cartilage was unclear. (4) Expression of heme oxygenase and level of collagen: at 6 weeks after injection, the expression of heme oxygenase in the experimental group was higher than that in the control group and blank control group (P < 0.05). The levels of collagen in the experimental group at 1, 3 and 6 weeks after injection were higher than those in the control group and the blank control group (P < 0.05). To conclude, hyaluronic acid injectable material loaded with chondrocytes can promote the repair of cartilage defects in rats.
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    Tissue-engineered cartilage construction by Cultispher microcarrier for articular cartilage repair
    Chang Bin, Xiao Tong-guang
    2017, 21 (30):  4793-4798.  doi: 10.3969/j.issn.2095-4344.2017.30.007
    Abstract ( 571 )   PDF (1277KB) ( 323 )   Save
    BACKGROUND: Both Cultispher microcarrier and fibrin can act as carriers in cartilage tissue engineering, but their application is limited by poor mechanical properties and poor plasticity.
    OBJECTIVE: To combine Cultispher microcarrier carrying chondrocytes with fibrin glue to construct Gultispher/fibrin composite scaffold, and to investigate the effect of this composite scaffold in the articular cartilage repair in a rabbit model.
    METHODS: Rabbit chondrocyte and Cultispher microcarriers were co-cultured in a stirred bioreactor until the chondrocytes adhered to and proliferated quickly on the microcarrier surface. Chondrocytes-seeded microcarries were then combined with fibrin glue to construct microcarrier/fibrin glue composite scaffolds, to repair trochlear cartilage defects of the knee joint in the rabbit model. In the experiment, three different treatments were respectively done for repair of cartilage defects, including implantation of chondrocytes-seeded microcarries/fibrin glue composite scaffold (MCF group), implantation of Gultispher/fibrin composite scaffold (MF group), and no treatment (blank control group). At 3 and 6 months after surgery, gross observation, histological evaluation, pathological evaluation and Micro-CT scanning were conducted to evaluate the cartilage repair effects.
    RESULTS AND CONCLUSION: Gross observation showed that the MCF group achieved better effect on cartilage repair, compared to the other two groups. Histopathological evaluation revealed hyaline-like cartilage tissues in the MCF group while ?brocartilage tissues were seen in the other two groups, shown by safranin O staining and sirius red staining. Micro-CT scanning results showed better subchondral bone remodeling was found in the MCF group than the other two groups. Gross observation and pathological observation showed better outcomes in the MCF group than the MF and blank control groups. To conclude, the chondrocyte-seeded Cultispher microcarrier/fibrin glue composite scaffold succeeds in the articular cartilage repair.
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    Effect of light-cured adhesives on color relapse after cold light tooth whitening 
    Xu Nuo, Shao Miao-miao, Hu Shu-hai, He Jian-ya, Wang Jun-ling, Li Xiao-jie
    2017, 21 (30):  4799-4804.  doi: 10.3969/j.issn.2095-4344.2017.30.008
    Abstract ( 464 )   PDF (1265KB) ( 234 )   Save
    BACKGROUND: Most studies suggest tooth whitening can create a coarse enamel surface which is likely to attract exogenous dyeing material and form the phenomenon of color relapse. After dental bleaching procedure, the use of resin surface treatment agent is expected to make the enamel surface smooth and alleviate tooth color relapse.
    OBJECTIVE: To unravel the efficacy of Adper Single Bond 2 Adhesive on color relapse phenomenon after dental bleaching treatment by cold light (xenon laser) whitening.
    METHODS: Twenty-four extracted teeth were collected and coated with 35% hydrogen peroxide followed by xenon laser whitening. The test group was made up of 12 samples coated with light-cured resin surface treatment agent and the left samples as control group were treated with no-coating agent after tooth whitening. Then the two groups were divided into two subgroups which were soaked in distilled water and tea for aging test respectively. The color differences (?E) which provided comparative values for statistical analysis was recorded at 1, 3, 5, 7, 14, 21, 28, 35 and 42 days after the aging test. The enamel surface microstructure of the samples was observed before and after bleaching, after resin agent coating, and 28 days after the aging test.
    RESULTS AND CONCLUSION: (1) Results from color differences observation: there was no significant difference between samples soaked in distilled water before and after whitening treatment (P > 0.05). Similarly, there was no statistical difference between test group and control group soaked in distilled water (P > 0.05). However, after soaking in tea, the color differences in the test group at 1-42 days showed statistically significant differences from those in the control group (P < 0.05). (2) Results from scanning electron microscope observation: after the tooth whitening producers, the enamel surface was damaged in the presence of cavities. After coated with resin surface treatment agent, the enamel surface became smooth and had few cavities. After soaking in tea, gradient smooth surface, some crack, inconspicuous flake dyeing color layer decomposition could be seen in the test group while rough surface with big holes and mottled dyeing layer were clearly visible in the control group. All samples soaked in distilled water had only a small amount of block dyeing layer, holes, weaker roughness than those soaked in tea. To conclude, Adper Single Bond 2 Adhesive light-cured resin surface treatment agent could weaken color relapse phenomenon after tooth whitening, achieving a smooth enamel surface and reducing dyeing material adhesion.
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    A comparative study on the mechanical properties of flexible denture materials containing different fluoride concentrations
    Tang Zhen-yu, Zhang Zhe, Zhang Wei-yi
    2017, 21 (30):  4805-4810.  doi: 10.3969/j.issn.2095-4344.2017.30.009
    Abstract ( 353 )   PDF (1036KB) ( 372 )   Save
    BACKGROUND: Long-term use of flexible denture prosthesis will increase the incidence of caries and periodontal disease, and partial denture stomatitis. The addition of fluoride in the prosthesis material can prevent and reduce the incidence of these diseases. Currently, increasing research addresses the addition of fluoride into dental materials at home and abroad, but little is reported on the addition of sodium monofluorophosphate (Na2(PO3)F into flexible denture materials and on the relevant mechanical performance.
    OBJECTIVE: To evaluate the mechanical properties of flexible denture materials containing different fluoride concentrations.
    METHODS: Totally 180 elastic denture samples were made at the three different sizes of 80 mm×51 mm×1 mm (n=60), 20 mm×5 mm×5 mm (n=60), 25 mm×2 mm×2 mm (n=60). Then, elastic denture samples containing Na2(PO3)F 0 (control), 3%, 5%, 10%, 15%, 20% were prepared in each size groups. The tensile strength, flexural strength and Shore A hardness of the samples were tested.
    RESULTS AND CONCLUSION: When the mass ratio of fluoride was up to 10%, the tensile strength and flexural strength reduced a little (P > 0.05). When the mass ratio of fluoride was up to 15%, the tensile strength and flexural strength were significantly lower than those of non-fluoride samples (P < 0.05). When the mass ratio of fluoride was up to 20%, the tensile strength, flexural strength and Shore A hardness were significantly lower than those of non-fluoride samples (P < 0.05). To conclude, Na2(PO3)F content has a significant effect on the tensile strength, flexural strength and Shore A hardness, and 10% is the best proportion of Na2(PO3)F. The mechanical properties of fluoride-containing flexible denture materials will be changed if the mass ratio of fluoride is higher than 10%.
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    Short-term effect of titanium abutment versus zirconia abutment on peri-implant tissues in anterior teeth
    Li Gan, Tong Xin, Qin Hai-yan, Wang Peng-lai
    2017, 21 (30):  4811-4816.  doi: 10.3969/j.issn.2095-4344.2017.30.010
    Abstract ( 413 )   PDF (1364KB) ( 325 )   Save
    BACKGROUND: With the increasing aesthetic pursuit of patients, an aesthetic abutment with certain strength has aroused people’s attentions either in the anterior or posterior tooth repair.
    OBJECTIVE: To evaluate the effects of titanium abutment and zirconia abutment on peri-implant tissue in anterior teeth.
    METHODS: A total of 40 anterior teeth from 34 patients were selected and received single crown restoration with Ankylos implants. Among them, titanium abutments were used in 20 teeth, and zirconia abutments in the other 20 teeth. All the patients were re-visited at 12 months after crown restoration. Peri-implant marginal bone resorption length (MBL), periodontal probing depth (PD), modified plaque index (mPLI), modified sulcus bleeding index (mSBI), and papilla index (PP) were detected; patient satisfaction investigation was carried out; and aesthetic assessment was done based on the pink esthetic score (PES).
    RESULTS AND CONCLUSION: There were no differences between two groups in MBL, PD, mPLI, mSBI, PP. Patients in both groups were satisfied with the treatment outcomes with insignificant difference (P > 0.05). The PES value in the zirconia abutment group was significantly higher than that in the titanium abutment group (P < 0.05). Overall, titanium and zirconia abutments show the similar effects on the peri-implant tissues and both can achieve good clinical outcomes. However, zirconia abutments have certain advantages in the aesthetic restoration for anterior teeth, and further investigation on long-term effects is warranted. 
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    A three-dimensional finite element model of Physiologic Anchorage Spee’s-wire System combined with opening bite by intrusion arch and analysis of relevant data
    Liu Guang-mei, Liu Hai-xia, Wu Xiao-ya, Wang Wei
    2017, 21 (30):  4817-4822.  doi: 10.3969/j.issn.2095-4344.2017.30.011
    Abstract ( 401 )   PDF (1467KB) ( 263 )   Save
    BACKGROUND: Physiologic Anchorage Spee’s-wire System (PASS) is a new appliance for orthodontic treatment in recent years. It has its superiority in support control, torque domination and friction control, and it is also unique in opening bite.
    OBJECTIVE: To build a three-dimensional finite element model of PASS with intrusion arch, and to analyze its effects on intrusion of the anterior teeth and on enhancing anchorage of the molars.
    METHODS: The CT scan data were imported into software of Mimicsl7.0 to obtain a three-dimensional model of the teeth and jaw bone. A series of modeling software such as Geomagic Studio, NX, ANSYSl5.0 were used to obtain the three-dimensional model of the maxillary dentition with PASS and intrusion arch. The overall force of the dentition and movement of the anterior teeth were analyzed when the intrusion arch was located at the mesial side of the central incisor and lateral incisor as well as the distal side of the lateral incisor.
    RESULTS AND CONCLUSION: It was efficient to accomplish the intrusion of the anterior teeth when the arch was located between central incisors. Labial inclination of the anterior teeth was achieved when the arch was ligated at the mesial side of the lateral incisor. Lingual inclination and intrusion of the anterior teeth was found when the arch was located at the distal side of the lateral incisor. Under these three kinds of conditions, the trend of molar retrodisplacement became remarkable. The maximum movement trend of the incisors was achieved, when the arch was ligated at the mesial side of the central incisor, mainly due to the intrusion of the incisors. The molar retrodisplacement was improved under the three conditions. This experiment simulated the stress distribution and displacement trend of the teeth, which is beneficial to the clinical understanding and application of the PASS technology with intrusion arch.
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    Stress distribution of the three-dimensional post-crown model with labial and lingual oblique defects of the maxillary central incisor: a finite element analysis
    Cai Yue, Huang Ying, Zhang Hui, Guo Ling
    2017, 21 (30):  4823-4829.  doi: 10.3969/j.issn.2095-4344.2017.30.012
    Abstract ( 383 )   PDF (1627KB) ( 214 )   Save
    BACKGROUND: Clinically, dentin ferrule has great influence on resistance, retention and edge sealing of post-core crowns.
    OBJECTIVE: To analyze the stress distribution of a three-dimensional post-crown model with labial and lingual oblique defects of the maxillary central incisor by finite element analysis.
    METHODS: A standard maxillary central incisor was selected and scanned by cone beam CT, and then, scanning data were imported to Mimics17 software. The CAD and UG software were used to construct the post-crown model with labial and lingual oblique defects. The dentin ferrule height of the defective side was designed to be 0 mm, and according to the dentin ferrule height at labial side-the dentin ferrule height at lingual side, there were the following groups: 0 mm-   0 mm, 0 mm-0.5 mm, 0 mm-1.0 mm, 0 mm-1.5 mm, 0 mm-2.0 mm, 0.5 mm-0 mm, 1.0 mm-0 mm, 1.5 mm-0 mm,   2.0 mm-0 mm, 0.5 mm-0.5mm, 1.0 mm-1.0 mm, 1.5 mm-1.5 mm, 2.0 mm-2.0 mm). In the crown cut 1/3, 1/3 at the junction, the Von Mises stress distribution of the root dentin and fiber post was analyzed by finite element analysis using a force of 100 N at 45° to the long axis of the tooth
    RESULTS AND CONCLUSION: (1) Root dentin stress distribution: In the case that the dentin ferrule at both labial and lingual sides was complete, with the increasing of the dentin height, the root Von Mises stress from the focus on the neck gradually became more evenly distributed and the peak stress gradually decreased. In the case of defective dentin ferrule, the peak of the Von Mises stress which had the dentin ferrule at the lingual side or the labial side was lower than that without the dentin ferrule, but at the same height, the dentin ferrule’s stress reduction at the lingual side was more obvious, close to the dentin ferrule ferrule complete group. (2) Fiber post stress distribution: in the case that the dentin ferrule at both labial and lingual sides was complete, with the increasing of the dentin height, fiber post’s Von Mises stress was gradually reduced. In the case of defective dentin ferrule, fiber post’s Von Mises stress which had dentin ferrule at the labial or lingual side was lower than that without the dentin ferrule, but at the same height, the dentin ferrule’s stress reduction at the labial side was more obvious. Overall, the lingual ferrule is more obvious on reducing root stress concentration than the labial ferrule, and the lingual ferrule height is beneficial to enhancing tooth resistance; the labial ferrule is more obvious on reducing the peak stress of fiber post and luting cement than the lingual ferrule, and the labial ferrule height is beneficial to increasing the retention and stabilization of the fiber post-core crown, and the effect becomes more obvious with at least 0.5-1.0 mm labial ferrule.
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    Three-dimensional finite element analysis of the cantilever fixed bridge supported by implants with different shapes 
    Wang Jun-zheng, Wang Qi-bo, Ding Chao, Shi Jiu-hui
    2017, 21 (30):  4830-4835.  doi: 10.3969/j.issn.2095-4344.2017.30.013
    Abstract ( 313 )   PDF (3579KB) ( 197 )   Save
    BACKGROUND: The shape of the implant exerts an effect on its biomechanics. At present, the research of implant-supported fixed partial denture with cantilever extension mainly focuses on the same shape, and little is reported on the comparison and analysis of implant-supported fixed partial denture with cantilever extension supported by different shape implants.
    OBJECTIVE: To compare the biomechanical behaviors of cantilever fixed bridges which were supported by three different implants, including cylindrical implant, tapered implant, expandable implant, in the mandibular posterior region with the help of three-dimensional finite element analysis.
    METHODS: The three-dimensional finite element models of the cantilever fixed bridges which were supported by cylindrical implant, tapered implant and expandable implant and their surrounding tissue in the mandibular posterior region were established were established. The force of 300 N was applied to the cantilever fixed bridges with axial 90° and buccolingual 45° to evaluate the maximum von Mises stress (Max EQV stress) of cortical bone and cancellous bone and the maximum displacement in implant-abutment complex.
    RESULTS AND CONCLUSION: Under axial and buccolingual loads, the Max EQV stress in the cortical bone was higher than that in the cancellous bone. The cantilever fixed bridge which was supported by expandable implant had the lowest Max EQV stress in the cortical bone, especially under axial load, and exhibited the highest Max EQV stress in the cancellous bone. The cantilever fixed bridges supported by three different implants showed an increase in the Max EQV stress of the cortical bone and cancellous bone and the maximum displacement in implant-abutment complex under buccolingual load. The cantilever fixed bridge which was supported by expandable implant had the minimum maximum displacement in the buccolingual direction. To conclude, the cantilever fixed bridge which is supported by expandable implant has best stability.
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    Platelet-rich fibrin combined with collagen membrane for repairing calvarial bone defects 
    Xie Jing, Wu Qian-qian, Sun Song, Sun Yong, Zhao Feng
    2017, 21 (30):  4836-4841.  doi: 10.3969/j.issn.2095-4344.2017.30.014
    Abstract ( 269 )   PDF (8634KB) ( 197 )   Save
    BACKGROUND: At present, there are some studies on applying platelet-rich fibrin (PRF) or collagen membrane in guided bone regeneration to promote the bone defect repair, while there is no report about their combined use versus single use in the bone defect repair.
    OBJECTIVE: To compare the bone regeneration ability of PRF, collagen membrane and their combined membrane in the bone defect repair.
    METHODS: Twenty-two Japanese big ear rabbits were selected to establish three bone defects in the calvarial bone, and then PRF, collagen membrane and PRF/collagen membrane composite were respectively implanted into the three defect regions. At 2, 4, 8, 12 weeks after implantation, tissue repair and regeneration in the bone defect regions were observed by X-ray and hematoxylin-eosin staining.
    RESULTS AND CONCLUSION: (1) Postoperative 2-week X-ray showed blurred density increase in the margin of bone defect in the composite membrane group, as well as increased density in the PRF group, while it was rarely seen in the collagen membrane group. At 12 weeks after implantation, in the composite membrane group, the bone density in the defect area was similar to the surrounding bone tissue; in the collagen membrane group, annulus-shaped density was enlarged, but the density in the defect region was still lower than that in the surrounding bone tissues; and in the PRF group, the lower density was seen in the individual parts of bone defect region compared with the surrounding bone tissues. (2) Histological observation: At 2 weeks after implantation, new fibrous connective tissues and newborn capillaries were seen around the defect area in the composite membrane group, while less fibrous connective tissues and capillaries were found in the other two groups. At 12 weeks after implantation, in the composite membrane group, a great amount of bone cells arranged regularly in the bone defect area with the presence of thickened trabecular bone and mature bone formation; in the PRF group, there were visible bone cells and increased number of trabecular bone, but less bone formation than the composite membrane group; in the collagen membrane group, there were few bone tissues formed with osteoblasts, osteoclasts and obvious bone lacuna. In conclusion, the PRF/collagen composite membrane shows better osteogenic effects than their use alone. 
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    Fresh amniotic membrane versus acellular amniotic membrane for repair of the tendon sheath and prevention of tendon adhesion
    Bai Jiang-bo, Zhao Hong-fang, Gao Rui-jiao, Zhang Bing, Yu Kun-lun, Yang Yan-tao, Ma Tao, Tian De-hu
    2017, 21 (30):  4842-4846.  doi: 10.3969/j.issn.2095-4344.2017.30.015
    Abstract ( 359 )   PDF (6605KB) ( 222 )   Save
    BACKGROUND: Amniotic membrane has a unique structure that can block the penetration of certain substances, to ensure normal nutrition supply for the surrounded tissues, and is also characterized by anti-adhesion, good tissue compatibility, mild inflammatory reaction, few fibers and biodegradability.
    OBJECTIVE: To compare the effects of fresh amniotic membrane and acellular amniotic membrane to prevent adhesion and promote tendon healing during the repair of tendon sheath defects.
    METHODS: Sixty healthy male leghorn chickens were selected, and the model of tendon injury and tendon sheath defects was established at the third toes. The animal models were then randomly divided into three groups and underwent repair with fresh amniotic membrane (group A), acellular amniotic membrane (group B), and no treatment in control group (group C), respectively. Histological observation and biomechanical analysis of the third toes were performed after repair.
    RESULTS AND CONCLUSION: (1) Histological observation. Congestive edema and inflammatory response were found in all animals at 2 weeks after repair, but mildest in the group A and severest in the group C. These inflammatory responses gradually alleviated over time in the three groups. At 12 weeks after repair, the new tendon sheath formed in all the animals, which was more mature than that at 4 weeks after repair. The synovial cells on the surface of the tendon sheath were arrayed tidily with dense structure in the groups A and B, but in the group C, the synovial cells were distributed disorderly with loose structure and prominent fibrous tissues. (2) Biomechanical analysis. Tendon sliding distance in the groups A and B was significantly larger than that in the group C at 4, 8, 12 weeks after repair (P < 0.05), but there were no significant difference in the distance between the groups A and B (P > 0.05). At 4 and 8 weeks after repair, the maximum tensile strength was largest in the group A, sequentially followed by group B and group C (P < 0.05), but there were no significant difference among the three groups at 12 weeks after repair (P > 0.05). To conclude, both fresh amniotic membrane and acellular amniotic membrane can promote tendon healing and prevent the adhesion of tendon through tendon sheath reconstruction, but the fresh amniotic membrane is preferred to promote early tendon healing compared with acellular amniotic membrane.
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    Autologous periosteum patch enhances tendon-bone healing in rotator cuff repair
    Li Jia, Dai Hai-feng, Liu Sha-sha, Xu Cong, Zhao Yang
    2017, 21 (30):  4847-4751.  doi: 10.3969/j.issn.2095-4344.2017.30.016
    Abstract ( 445 )   PDF (4625KB) ( 278 )   Save
    BACKGROUND: The latest research shows that the rotator cuff patch can reduce tendon-bone tension, provide the point of attachment for growth factor and cell growth, and promote biological reconstruction of the rotator cuff, but what kind of material as the rotator cuff patch is the most effectively and safety is unclear.
    OBJECTIVE: To investigate the effect of autologous periosteum patch on the tendon-bone healing in rotator cuff repair.
    METHODS: Forty-eight New Zealand white rabbits were selected to make acute unilateral rotator cuff injury models and then randomly divided into two groups: control group treated with simple suture repair and experimental group treated with autologous periosteum patch on the basis of suture repair. At 4, 8, 12 weeks after repair, proximal humerus specimens were taken, and the histological observation and biomechanical testing were recorded.
    RESULTS AND CONCLUSION: (1) Histological observation: At 4 weeks after repair, the experimental group showed a large amount of interconnected collagen fibers and infiltrated inflammatory cells and a small amount of cartilage cells, and mesenchymal stem cells proliferated remarkably. At 8 weeks after repair, the experimental group showed a large amount of cartilage cells. At 12 weeks, in the experimental group, the tendon bone interface was similar to normal with regular arrangement of cartilage cells. In the control group, loose connective tissues were seen at 4 weeks; a large amount of fibroblasts appeared at the ending point, a small amount of collagen fibers were connected and a small amount of chondrocytes were formed at 8 weeks after repair; collagen fibers were connected and chondrocytes were disordered at 12 weeks after repair. (2) Biomechanical test: The maximum load of the two groups increased gradually with time, and the maximum load of the experimental group was higher than that of the control group at different time points (P < 0.05). To conclude, the autologous periosteum patch can effectively promote the tendon-bone healing in rotator cuff repair.
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    Therapeutic effect of adipose derived mesenchymal stem cell conditioned medium thermosensitive hydrogel on skin scald
    Liu Pei, Hu Zhen-sheng, Ma Ling, Wang Huan-huan, Li Dong
    2017, 21 (30):  4852-4859.  doi: 10.3969/j.issn.2095-4344.2017.30.017
    Abstract ( 497 )   PDF (6395KB) ( 322 )   Save
    BACKGROUND: Studies have shown that adipose derived mesenchymal stem cells are involved in the skin repair after scald, but the hydrogel made of the excreta by adipose derived mesenchymal stem cells is rarely reported in the treatment of skin scald.
    OBJECTIVE: To analyze the therapeutic effect of human adipose derived mesenchymal stem cell conditioned medium hydrogel in a mouse model of skin scald.
    METHODS: Adipose derived mesenchymal stem cells were obtained from adipose tissues by enzyme digestion combined with adherent culture method. Morphological and flow cytometry were used to identify phenotype and induce differentiation. Secondly, the stable proliferative phase cells were harvested to obtain the conditioned medium, and chitosan, mannitol, beta-glycerol phosphate sodium and hyaluronic acid were added to prepare the thermosensitive hydrogel. Then the 95 ℃ aluminum block was used to rapidly establish a model of degree III skin scald on the left (experimental group) and right (control group) sides of the back of 24 C57BL/6 mice. In the experimental group, adipose derived stem cell conditioned medium hydrogel was applied twice a day on the right side of the mouse back, and in the control group, fresh medium hydrogel was applied twice a day on the left side of the mouse back. The treatment period lasted for 7 days. Healing time and healing process were observed to calculate the healing rate. Histopathological changes were observed by hematoxylin-eosin staining at paraffin sections at 4, 14, 28 days after skin scald.
    RESULTS AND CONCLUSION: (1) The human adipose derived mesenchymal stem cells had fibroblast-like morphology and proliferated vigorously, and the average doubling time was 55 hours. These cells could be induced to differentiate into osteoblasts, chondrocytes and adipocytes. High expression of CD29, CD44, CD90 and CD105 were observed on these cells with low expression of CD31 and CD34, which met the standard of mesenchymal stem cells. (2) The thermosensitive hydrogel prepared by the conditioned medium was cool and transparent viscous liquid at 4-20 ℃, and was changed into semi-solid gel at 37 ℃ after 15 minutes. (3) The normal structures of the epidermis, dermis, subcutaneous fat and muscle tissue of 95 ℃ aluminum block scalded mice were completely destroyed, which met the standards of degree III burns. The wound area was roughly 3 cm2. (4) In the repair process, shorter wound healing time, less scar and better dermis structure were observed in the experimental group compared with the control group. (5) Inflammatory infiltration, thickness of granulation tissue, epidermal thickness, fibroblasts and vascular density were significantly improved in the experimental group as compared with the control group (P < 0.05). To conclude, human adipose derived mesenchymal stem cells conditioned medium hydrogel can promote the wound healing and promote the quality of regenerated skin after skin scald. 
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    Preparation and in vitro properties of degradable antibacterial composite hydrogel for intestinal fistulas
    Niu Yu-xiang, Xiong Dang-sheng
    2017, 21 (30):  4860-4866.  doi: 10.3969/j.issn.2095-4344.2017.30.018
    Abstract ( 331 )   PDF (1854KB) ( 261 )   Save
    BACKGROUND: As the surgical treatment for intestinal fistulas nowadays needs to be improved, we are seeking a new kind of artificially synthesized hydrogel to replace fibrin gels as the sealing gel, which is imperative for both economic and social benefits.
    OBJECTIVE: To prepare a degradable antibacterial composite hydrogel and to detect the in vitro biological properties.
    METHODS: In this study, we combined soluble chitosan (S-Cts) with oxidized alginate (O-Alg) to prepare the injectable and degradable hydrogel under Schiff base reaction. Besides, nanosilver (nano-Ag) particles were added to obtain S-Cts/O-Alg/nano-Ag composite hydrogel. Gelation time, microstructure, swelling, degradation, and antibacterial properties of the composite hydrogel were observed and detected in simulated physiological environment.
    RESULTS AND CONCLUSION: The closer constituent contents of water-soluble chitosan and sodium alginate indicate the shorter gelation time, and the time could be controlled within the range of surgery. The variation in the constituent content of the two components can affect the hydrogel microstructure. The higher constituent content of water-soluble chitosan implicates the denser network of the hydrogel. The composite hydrogel has excellent swelling properties, and it degrades faster in the simulated intestinal fluid containing trypsin than in the PBS. Moreover, adding nanosilver particles can bring certain antibacterial properties. This hydrogel has better biocompatibility, biodegradability and antibacterial ability than natural macromolecules, and has certain research value and application prospect.
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    Performance characterization of bacterial cellulose reinforced polyvinyl alcohol/ polyacrylamide composite hydrogels
    Ma Biao, Dong Yan-yan, Chen Shi-wen
    2017, 21 (30):  4867-4872.  doi: 10.3969/j.issn.2095-4344.2017.30.019
    Abstract ( 479 )   PDF (1160KB) ( 223 )   Save
    BACKGROUND: With the extension of the application of polymer hydrogel materials, to develop polymer hydrogel materials with better performance has become a hotspot.
    OBJECTIVE: To compare the mechanical properties of polyvinyl alcohol/polyacrylamide (PVA/PAM) composite hydrogel before and after modification by bacterial cellulose (BC), thereby providing a theoretic basis for its application in wound dressing.  
    METHODS: BC-reinforced PVA/PAM hydrogels with different PVA/PAM contents were prepared using repeated freezing-thaw method. The BC/PVA/PAM hydrogels were characterized by infrared spectrum (IR) analysis, mechanical property test and scanning electron microscopy (SEM) observation, and thermogravimetric analysis.
    RESULTS AND CONCLUSION: SEM and IR results showed the PVA and PAM were combined and attached onto the surface of BC, indicating a success in the preparation of BC/PVA/PAM hydrogels. Results from the thermogravimetric analysis showed that the thermal-stability of the BC/PVA/PAM hydrogels improved significantly. The mechanical test results demonstrated that the tensile strength of the PVA/PAM hydrogels was strongly enhanced, and the elongation at break was significantly lowered after being reinforced with BC. Furthermore, the content of PVA and PAM had an obvious effect on the mechanical properties of the composites. When the PAM content was 1.0%, the tensile strength and elongation at break of BC/PVA/PAM composites were at the highest level, which were 331.79 kPa and 105.33%, respectively; when the PVA content was 3.5%, the elongation at break of the BC/PVA/PAM composites was increased to the maximum (46.25%). Moreover, the results of this work reveal that the BC/PVA/PAM hydrogels exhibit some promising characteristics as artificial biomaterials. In general, the introduction of bacterial cellulose significantly improves the mechanical properties of the PVA/PAM hydrogels, and enhances the stability of the BC/PVA/PAM.
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    Hemostatic effect of collagen sponge on liver trauma
    Zhang Zi-qiang, Zhang Yi-he, Zu Yao-xian, An Qi, Xu Rong-rong
    2017, 21 (30):  4873-4878.  doi: 10.3969/j.issn.2095-4344.2017.30.020
    Abstract ( 438 )   PDF (1300KB) ( 280 )   Save
    BACKGROUND: iRegene collagen sponge exhibits stable physical and chemical properties, and has passed the test by the State Food and Drug Administration of China.
    OBJECTIVE: To study the hemostatic effect and the biocompatibility of the iRegene collagen sponge on a liver wound by means of rat models.
    METHODS: Liver trauma bleeding models were made in 21 Sprague-Dawley rats. These model rats were randomized into three groups (n=7 per group): experimental group with implantation and external application of iRegene collagen sponge; positive control group with implantation of medical collagen sponge and external application of iRegene collagen sponge; blank control group with external application of medical gauze. The bleeding time and amount on the liver wounds were observed. Histological observation on the liver wound was performed at 7, 14, 28 days after intervention.
    RESULTS AND CONCLUSION: The bleeding time was shorter in the experimental group than the positive control group (P ≤ 0.05). Beyond that, there was no difference in the bleeding amount and time among the three groups. Histological findings on the liver wound showed that the iRegene collagen sponge in the experimental group was completely wrapped with fibrous connective tissues and began to degrade at 7 days after intervention, the Inflammatory cell infiltration mainly occurred in neutrophils, and new capillaries were observed in peripheral connective tissues; at 14 days after intervention, the fibrous connective tissues became remarkably thickened, the number of neurophils was reduced, and the number of macrophages was increased; at 28 days after intervention, the iRegene collagen sponge degraded completely, most of the liver tissues recovered, and there were macrophages, monocytes, fibroblasts and capillaries in the inflammatory connective tissues adjacent to a part of liver tissues. Similar findings were observed in the positive control group. In the blank control group, there were obvious connective tissues on the wound and red blood cells in the liver sinus, and occasionally liver tissue bleeding and vacuolar degeneration were visible; at 28 days after intervention, there were thickened connective tissues on the wound, red blood cells in the liver sinus and reversed hepatic stellate cells. To conclude, the iRegene collagen sponge possesses effective hemostatic effects on liver wounds and shows good histocompatibility.
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    Arthroscopic posterior cruciate ligament reconstruction using autologous and allogeneic materials and the association with matrix metalloproteinases 2

    Tang Ju, Lou Fang-yong, Zhu Wei, Jiang Hai-tao, Zhang Zhen-xiang
    2017, 21 (30):  4879-4884.  doi: 10.3969/j.issn.2095-4344.2017.30.021
    Abstract ( 340 )   PDF (1106KB) ( 205 )   Save
    BACKGROUND: Self-healing ability of the posterior cruciate ligament is poor, and the degradation and synthesis of extracellular matrix often follow the ligament repair. The matrix metalloproteinase family plays a critical role in the dynamic equilibrium between the matrix degradation and synthesis.
    OBJECTIVE: To investigate the effect of arthroscopic reconstruction of the posterior cruciate ligament, and to study its correlation with matrix metalloproteinase 2 level.
    METHODS: Sixty patients with posterior cruciate ligament rupture were studied, including 37 cases of autologous reconstruction and 13 cases of allogeneic reconstruction. Ligament and synovial cells from traumatic amputation patients with no ligament injury in the corresponding period were collected. Lysholm and Tegner scores were detected before and after operation. The results of postoperative drawer test were analyzed. The tibial displacement of the posterior cruciate ligament after autologous reconstruction and allogeneic reconstruction was compared. The posterior cruciate ligament cells were cultured alone or co-cultured with synovial cells, and then the level of matrix metalloproteinase 2 protein was detected. In addition, operation time, incision length, postoperative fever time and gender differences were also detected and compared.
    RESULTS AND CONCLUSION: Tibial displacement, irrespective of genders, was higher in the allogeneic reconstruction group than the autologous reconstruction group, while there were no significant differences in the posterior drawer test between the two reconstruction groups as well as between males and females. Postoperative Lysholm and Tegner scores were both improved significantly (P < 0.01). As time went by, the level of matrix metalloproteinase 2 had an increasing trend in the posterior cruciate ligament cells cultured alone or co-cultured with synovial cells, but the level in the co-culture group was higher than that in the single culture group. For both male and female, the autologous reconstruction group showed a longer operative time (P < 0.05 or 0.01) and a longer incision length (P < 0.01), as compared with the allogeneic reconstruction group, while the time of fever was significantly longer in the allogeneic reconstruction group (P < 0.01). Results from the last follow-up show that the autologous reconstruction is better than the allogeneic reconstruction to restore the stability of posterior cruciate ligament and shorten fever time, but longer operative time and surgical incision as well as increased level of matrix metalloproteinase 2 cannot be ignored. 
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    Application of chitosan-based hydrogels carrying antimicrobial agents in wound healing  
    Li Zu-hao, Wang Chen-yu, Wang Zhong-han, Zhong Lei, Li Chen, Qin Yan-guo, Liu He, Wang Jin-cheng
    2017, 21 (30):  4885-4892.  doi: 10.3969/j.issn.2095-4344.2017.30.022
    Abstract ( 534 )   PDF (1728KB) ( 338 )   Save
    BACKGROUND: With the development of chemical synthesis technology, the application of chitosan-based hydrogels as wound dressing has become a hotspot in current research.
    OBJECTIVE: To generally introduce the chitosan-based hydrogels and to review and summarize the application of chitosan-based hydrogels carrying antimicrobial agents in wound healing.
    METHODS: A computer retrieval of PubMed, Web of Science, Springerlink and Medline databases was performed with “chitosan hydrogel, wound healing, dressing, antimicrobial agents” as key words for relevant articles published from 2007 to 2016. Initially, 168 articles were retrieved, and finally 76 articles were included in result analysis.
    RESULTS AND CONCLUSION: Because of their inherent anti-inflammatory and antibacterial properties, chitosan-based hydrogels possess the ability of absorbing exudates and forming thin film between the wound and the external environment, to exert their advantages for chronic, non-healing wounds caused by infection. Furthermore, chitosan-based hydrogels carrying antibacterial agents can inhibit the growth of microorganisms in the wound, accelerating the progress of wound healing. Moreover, the antimicrobial agents, including organic antibacterial agents and inorganic antibacterial agents (especially nano-silver), show unique advantages in wound healing and microbial resistance in combination with chitosan-based hydrogels.
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    Application of chitosan scaffolds in tissue engineering
    Chen Dao-yu, Zhang Zhong-min, Jiang Li-li
    2017, 21 (30):  4893-4900.  doi: 10.3969/j.issn.2095-4344.2017.30.023
    Abstract ( 418 )   PDF (1566KB) ( 329 )   Save
    BACKGROUND: Chitosan as a functional polymer material has been widely used in the field of biomedicine, and it is an intelligent material with sensor function, feedback function and response function.
    OBJECTIVE: To discuss the recent application of chitosan scaffolds in different fields of tissue engineering, and to highlight the advantages and disadvantages of chitosan as a scaffold material.
    METHODS: The key words of "tissue engineering, scaffold, chitosan" in Chinese and English were used to search CNKI and PubMed databases for relevant articles published from 2000 to 2016. After the initial screening, the reserved articles were further detailed, summarized and concluded.
    RESULTS AND CONCLUSION: Due to its biological properties such as being biocompatible, biodegradable and carrying many different growth factors, chitosan has been widely used in the tissue engineering field for repair of different tissues including nerve tissue, periodontal tissue, bone tissue, and myocardial tissue. However, the application of chitosan as a scaffold in tissue engineering is limited due to its shortcoming. The combination of chitosan and other biocompatible materials provides a solution to these limitations, which greatly broadens the application of chitosan.
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    Repairing peripheral nerve injury with nerve conduits
    Chen Yong, Fan Lin, Fu Zhen, Xiong Yan, Wang Yan-feng, Ye Qi-fa, Qin Wei
    2017, 21 (30):  4901-4907.  doi: 10.3969/j.issn.2095-4344.2017.30.024
    Abstract ( 644 )   PDF (1121KB) ( 506 )   Save
    BACKGROUND: In recent years, the rapid development of medical and tissue engineering has provided more choices for making nerve conduit preparation.
    OBJECTIVE: To review the application of nerve conduits in the repair of peripheral nerve injury.
    METHODS: The first author retrieved the CNKI and PubMed databases to search relevant articles published from 2010 to 2016. The key words were "nerve conduit, peripheral nerve" in Chinese and English, respectively.
    RESULTS AND CONCLUSION: The nerve conduit materials are mainly classified into biotype and non-biotype. Biotype materials mainly include muscle, amniotic membrane, vein and small intestinal submucosal layer. The non-biotype materials include chitosan, collagen, silk fibroin, polylactic acid, polycaprolactone, polyaniline and silicone tubes. Some materials currently have been approved to enter the clinical stage. There are more or less problems in the clinical application of nerve conduits in the repair of peripheral nerve injury. For example, the length of a defect that can be repaired is limited; the mechanical properties and mechanical properties are not exactly matched with nerve regeneration; the degradation rate is inconsistent with the rate of nerve regeneration; and poor biocompatibility exists.
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    Mechanism and application of autologous platelet-rich plasma in the treatment of joint and sports injuries
    Fu Jia-xin, Wang Shu-sen
    2017, 21 (30):  4908-4914.  doi: 10.3969/j.issn.2095-4344.2017.30.025
    Abstract ( 568 )   PDF (1461KB) ( 241 )   Save
    BACKGROUND: Platelet-rich plasma is an autologous plasma product that is simple and easy to be obtained, and has been widely used in a variety of sports injuries, non-infectious tendon and ligament injuries, nonunion, bone healing, and cartilage repair, because it contains a large number of growth factors that promote tissue healing and soft tissue regeneration. However, its actual effects are uncertain.
    OBJECTIVE: To introduce the pathophysiological mechanism, preparation and preservation of autologous platelet-rich plasma and to review the research status of autologous platelet-rich plasma in the treatment of bone and joint injuries induced by sports.
    METHODS: A computer retrieval in WanFang, CNKI, and PubMed databases was performed for articles addressing clinical and laboratory reports on autologous platelet-rich plasma for diseases of bone and joint injuries published from 1990 to 2016. Then, we summarized the research status of autologous platelet-rich plasma in the treatment of bone and joint diseases and motor system damage.
    RESULTS AND CONCLUSION: The mechanism of autologous platelet-rich plasma to repair bone and soft tissue injuries is not yet fully understood, and there is still a lot of reasoning and speculation. Moreover, its dose, treatment course, administration route and method of use have not been fully standardized, and there are still no clear guidelines for its clinical use. Autologous platelet -rich plasma has a broad application prospect in the treatment of bone and joint injuries, but it is still in the initial exploratory stage, which needs further observation and summarization to get more powerful evidence. Up to now, there are few samples in the world, in the absence of effective control standards, and long-term large-sample rigorous research is required to reach the final conclusion.
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    Research progress in the stability of miniscrews as anchorage
    Chen Yan-qu, Tang Min, Huang Xuan-ping
    2017, 21 (30):  4915-4920.  doi: 10.3969/j.issn.2095-4344.2017.30.026
    Abstract ( 340 )   PDF (1058KB) ( 285 )   Save
    BACKGROUND: Miniscrews have been widely used as “absolute anchorage” in orthodontic treatment, but high shedding rate has been a major flaw. Factors associated with the miniscrew stability mainly include its own factors, surrounding inflammation, implant method, angle, position and loading time of miniscrews.
    OBJECTIVE: To review the factors affecting the stability of miniscrews reported at home and abroad in recent years.
    METHODS: The first author searched CNKI and PubMed using computer for relevant articles published in recent 4 years, and the keywords were “miniscrews, stability, orthodontic anchorage” in Chinese and English, respectively.
    RESULTS AND CONCLUSION: A total of 52 articles were included. The surface modification of miniscrews effectively enhances bone binding capacity, and promotes bone regeneration, thereby improving the stability of miniscrews. The morphology, implanted angle and mode, bone density and load timing of miniscrews are closely related to the stability of miniscrews. Meanwhile, we should avoid damage to the root, nerves, blood vessels and maxillary sinus, and focus on the maintenance of postoperative healing.
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