Therapeutic effect of adipose derived mesenchymal stem cell conditioned medium thermosensitive hydrogel on skin scald

doi:10.3969/j.issn.2095-4344.2017.30.017

Abstract

Abstract:

BACKGROUND: Studies have shown that adipose derived mesenchymal stem cells are involved in the skin repair after scald, but the hydrogel made of the excreta by adipose derived mesenchymal stem cells is rarely reported in the treatment of skin scald.

OBJECTIVE: To analyze the therapeutic effect of human adipose derived mesenchymal stem cell conditioned medium hydrogel in a mouse model of skin scald.

METHODS: Adipose derived mesenchymal stem cells were obtained from adipose tissues by enzyme digestion combined with adherent culture method. Morphological and flow cytometry were used to identify phenotype and induce differentiation. Secondly, the stable proliferative phase cells were harvested to obtain the conditioned medium, and chitosan, mannitol, beta-glycerol phosphate sodium and hyaluronic acid were added to prepare the thermosensitive hydrogel. Then the 95 ℃ aluminum block was used to rapidly establish a model of degree III skin scald on the left (experimental group) and right (control group) sides of the back of 24 C57BL/6 mice. In the experimental group, adipose derived stem cell conditioned medium hydrogel was applied twice a day on the right side of the mouse back, and in the control group, fresh medium hydrogel was applied twice a day on the left side of the mouse back. The treatment period lasted for 7 days. Healing time and healing process were observed to calculate the healing rate. Histopathological changes were observed by hematoxylin-eosin staining at paraffin sections at 4, 14, 28 days after skin scald.

RESULTS AND CONCLUSION: (1) The human adipose derived mesenchymal stem cells had fibroblast-like morphology and proliferated vigorously, and the average doubling time was 55 hours. These cells could be induced to differentiate into osteoblasts, chondrocytes and adipocytes. High expression of CD29, CD44, CD90 and CD105 were observed on these cells with low expression of CD31 and CD34, which met the standard of mesenchymal stem cells. (2) The thermosensitive hydrogel prepared by the conditioned medium was cool and transparent viscous liquid at 4-20 ℃, and was changed into semi-solid gel at 37 ℃ after 15 minutes. (3) The normal structures of the epidermis, dermis, subcutaneous fat and muscle tissue of 95 ℃ aluminum block scalded mice were completely destroyed, which met the standards of degree III burns. The wound area was roughly 3 cm². (4) In the repair process, shorter wound healing time, less scar and better dermis structure were observed in the experimental group compared with the control group. (5) Inflammatory infiltration, thickness of granulation tissue, epidermal thickness, fibroblasts and vascular density were significantly improved in the experimental group as compared with the control group (P < 0.05). To conclude, human adipose derived mesenchymal stem cells conditioned medium hydrogel can promote the wound healing and promote the quality of regenerated skin after skin scald.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Adipose Tissue, Mesenchymal Stem Cells, Culture Media, Conditioned, Hydrogel, Skin, Wounds and Injuries, Tissue Engineering

CLC Number:

R318

Liu Pei, Hu Zhen-sheng, Ma Ling, Wang Huan-huan, Li Dong.

Therapeutic effect of adipose derived mesenchymal stem cell conditioned medium thermosensitive hydrogel on skin scald

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(30): 4852-4859.

Figures/Tables 7

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