Effect of bone morphogenetic protein 7 adenovirus transfection on biological function of bone marrow stromal stem cells

doi:10.3969/j.issn.2095-4344.2015.06.004

Abstract

Abstract:

BACKGROUND: Gene transfer techniques have been actively used in tissue regeneration therapy. Bone morphogenetic protein 7 with osteoinductive properties can effectively promote osteoblast growth and new bone formation.
OBJECTIVE: To explore the influence of bone morphogenetic protein 7 adenovirus gene transfection on the biological function of bone marrow stromal stem cells.
METHODS: Goat bone marrow stromal stem cells were isolated, cultured and transfected by recombinant adenovirus containing bone morphogenetic protein 7 (Adeno-BMP7). Cell ultrastructure was observed by transmission electronic microscope, cell cycle was detected by flow cytometry, expression of bone morphogenetic protein 7 was measure by western blot assay, and Von Kossa staining was used to observe the formation of calcium nodules. Bone marrow stromal stem cells transfected for 3 days and untransfected were used to prepare coral-cell complexes that were injected subcutaneously into the back of nude mice for 4 weeks and 8 weeks, followed by gross observation and histological examination.
RESULTS AND CONCLUSION: Adeno-BMP7-transfected bone marrow stromal stem cells appeared to have active substance synthesis and metabolism. Adeno-BMP7 transfection played no effect on the cell cycle of bone marrow stromal stem cells. Bone morphogenetic protein 7 was expressed in the transfected bone marrow stromal stem cells. Larger calcium nodules were visible after Adeno-BMP7 transfection. Adeno-BMP7-transfected bone marrow stromal stem cells showed stronger osteogenic capability and higher bone quality. These results demonstrate that Adeno-BMP7 transfection can effectively promote the osteogenic differentiation of bone marrow stromal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cells, Bone Morphogenetic Protein 7, Genes

CLC Number:

R394.2

Pu Zhi-chao, Xie Wei-yong, Wang Yan-bin, Xue Jian, Zhang Xing-shi. Effect of bone morphogenetic protein 7 adenovirus transfection on biological function of bone marrow stromal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 837-842.

Figures/Tables 2

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