Detection of the calcium and phosphorus in the hard tissue of permanent teeth: spectrophotometry and ethylene diamine tetraacetie acid titration

doi:10.3969/j.issn.2095-4344.2014.46.011

Abstract

Abstract:

BACKGROUND: Currently, acid etching and bonding technology have been widely used in clinical stomatology. Data have indicated that the main content of inorganic elements (calcium and phosphorus) has a certain difference between the dental enamel and dentin of the young and adult permanent teeth.

OBJECTIVE: To measure the content of main inorganic elements, calcium and phosphorus, in the dental enamel and dentin from young and adult permanent teeth with spectrophotometry and ethylene diamine tetraacetie acid titration method.

METHODS: Each 20 adult and young permanent teeth in vitro were selected. Plaster stone and water was mixed; when it was nearly dried, the teeth were vertically cast in the mixture and the tooth surface was exposed. The models were cut into the slices using syj-200 precision cutting machine, and then the slices were put into nitric acid and dissolved through heater to prepare standard solution. At last, the concentrations of calcium and phosphorus in the hard tissue of both young and adult permanent teeth were measured with spectrophotometric method and titration method.

RESULTS AND CONCLUSION: The content of calcium and phosphorus and calcium/phosphorus ratio in the hard tissue of young permanent teeth were less than those of adult permanent teeth (P < 0.05), reflecting that the organic matter content was more than that in adult permanent teeth, but their mineralized degree was inferior to that of adult permanent teeth (P < 0.05). These findings indicate that the young permanent teeth are more acid proof than the adult permanent teeth; therefore, the acid etching time can be properly prolonged for young permanent teeth in clinical treatment, in order to achieve better effects.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: tissue engineering, dental enamel, dentin, calcium, phosphorus

CLC Number:

R318

Ma Hai-liang, Yu Xin-bo, Liu Meng-dong, Jia Jing, Fu Da-yong. Detection of the calcium and phosphorus in the hard tissue of permanent teeth: spectrophotometry and ethylene diamine tetraacetie acid titration[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(46): 7432-7436.

Figures/Tables 1

References

[1] Filipský T, Ríha M, Hrdina R, et al. Mathematical calculations of iron complex stoichiometry by direct UV-Vis spectrophotometry. 2013;49:1-8.
[2] Geerligs HJ, Meinders CA, Snel Y, et al. The use of RT-PCR for determination of separate end-points for the strains IB H120 and IB D274 in titration of the combination vaccine Poulvac IB® primer. J Virol Methods. 2013;193(2): 508-511.
[3] Sh P, Raghu R, Shetty A, et al. Effect of organic versus inorganic fluoride on enamel microhardness: an in vitro study. J Conserv Dent. 2013;16(3):203-207.
[4] Butt N, Talwar S. In-vitro evaluation of various solvents for retrieval of mineral trioxide aggregate and their effect on microhardness of dentin. J Conserv Dent. 2013;16(3): 199-202.
[5] Wan Bakar W, McIntyre J. Susceptibility of selected tooth-coloured dental materials to damage by common erosive acids. Aust Dent J. 2008;53(3):226-234.
[6] Shirban F, Khoroushi M, Shirban M. A new solvent-free one-step self-etch adhesive: bond strength to tooth structures. J Contemp Dent Pract. 2013;14(2):269-274.
[7] Fares J, Shirodaria S, Chiu K, et al. A new index of tooth wear. Reproducibility and application to a sample of 18- to 30-year-old university students. Caries Res. 2009;43(2): 119-125.
[8] Jung K, Miyagawa M, Matsuda A, et al. Antifungal effects of palmitic acid salt and ultrapure soft water on Scedosporium apiospermum. J Appl Microbiol. 2013;115(3):711-717.
[9] Kasraei S, Azarsina M, Khamverdi Z. Effect of Ethylene diamine tetra acetic acid and sodium hypochlorite solution conditioning on microtensile bond strength of one-step self-etch adhesives. J Conserv Dent. 2013; 16(3): 243-246.
[10] Kourkoumelis N, Balatsoukas I, Tzaphlidou M. Ca/P concentration ratio at different sites of normal and osteoporotic rabbit bones evaluated by Auger and energy dispersive X-ray spectroscopy. J Biol Phys. 2012;38(2): 279-291.
[11] Bolanos-Carmona V, Gonzalez-Lopez S, Briones-Lujan T, et al. Effects of etching time of primary dentin on interface morphology and microtensile bond strength. Dent Mater. 2006;22(12):1121-1129.
[12] Wang L, Yan M, Wang Y, et al. Proliferation and osteo/odontoblastic differentiation of stem cells from dental apical papilla in mineralization-inducing medium containing additional KH(2)PO(4). Cell Prolif. 2013;46(2):214-222.
[13] Engle K, Hara AT, Matis B, et al. Erosion and abrasion of enamel and dentin associated with at-home bleaching: an in vitro study. J Am Dent Assoc. 2010;141(5):546-551.
[14] Boskey AL, Verdelis K, Spevak L, et al. Mineral and matrix changes in brtl/+ teeth provide insights into mineralization mechanisms. Biomed Res Int. 2013;2013:295812.
[15] McKee MD, Yadav MC, Foster BL, et al. Compounded PHOSPHO1/ALPL Deficiencies Reduce Dentin Mineralization. J Dent Res. 2013;92(8):721-727.
[16] Lv RJ, Li YJ, Yao P. Histology of incipient “young” human enamel. Beijing Kouqiang Yixue. 2003;11(3):125-127.
[17] Veis A, Alvares K, Dixit SN, et al. Characterization of two distinctly different mineral-related proteins from the teeth of the Camarodont sea urchin Lytechinus variegatus: Specificity of function with relation to mineralization. Front Mater Sci China. 2009;3(2):163-168.
[18] Bishara SE, Soliman M, Laffoon J, et al. Effect of antimicrobial monomer-containing adhesive on shear bond strength of orthodontic brackets. Angle Orthod. 2005;75(3):397-399.
[19] Erhardt MC, Osorio E, Aguilera FS. Influence of dentin acid-etching and NaOCl-treatment on bond strengths of self-etch adhesives. Am J Dent. 2008;21(1):44-48.