Endothelial progenitor cell transplantation influences the inflammatory state in lung injury

doi:10.3969/j.issn.2095-4344.2013.10.012

Abstract

Abstract:

BACKGROUND: Endothelial progenitor cells play an important role in maintaining normal endothelial function as well as endothelial repairing after vascular injury, which has been widely applied to multiple clinical disorders including cardiovascular disease, limb ischemia and vascular repair. But the application studies of endothelial progenitor cells are much fewer in inflammatory diseases and lung injury.
OBJECTIVE: To observe the effect of endothelial progenitor cell transplantation on expressions of tumor necrosis factor-α and interleukin-10 in acute lung injury/acute respiratory distress syndrome rats and to explore whether cell transplantation can improve the inflammatory state of acute lung injury/acute respiratory distress syndrome.
METHODS: Thirty Sprague Dawley rats of the same genetic background were randomly divided into three groups with 10 rats in each group: normal control group, lung injury group and cell transplantation group. The endothelial progenitor cells were isolated and cultured with density gradient centrifugation method. Rats in the lung injury group and cell transplantation group were injected with lipopolysaccharide through tail vein to make the acute lung injury models. Rats in normal control group only received phosphate buffered solution with the same dose. Half an hour after modeling, rats in the normal control group and cell transplantation group were injected with bone marrow-derived endothelial progenitor cells suspension through tail vein, and rats in lung injury group received the same amounts of phosphate buffered solution.
RESULTS AND CONCLUSION: Compared with the lung injury group, the expression of interleukin-10 was significantly increased in the cell transplantation group (P < 0.001), but the expression of tumour necrosis factor-α was reduced, and the difference was not significant (P > 0.05). It indicated that transplantation of endothelial progenitor cells could up-regulate interleukin-10 expression and down-regulate tumour necrosis factor-α expression, and could significantly improve the inflammatory state in injury lung tissues.

Key words: stem cells, stem cell transplantation, endothelial progenitor cells, stem cell transplantation, tumour necrosis factor-α, interleukin-10, lung tissue, inflammation, other grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Mao Mei, Zhang Bo, Fu Zu-hong, Xu Xi-lin, Zhang Yong. Endothelial progenitor cell transplantation influences the inflammatory state in lung injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(10): 1780-1784.

Figures/Tables 5

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