Impact of different preparation methods for graft materials on biological properties of allogeneic tendon

doi:10.3969/j.issn.2095-4344.2014.39.020

Abstract

Abstract:

BACKGROUND: The transplanted tendon must have good biomechanical properties, in order to effectively avoid tendon tear at the anastomosis end during suturing and reduce adhesion of tendon during healing process.

OBJECTIVE: To investigate the effects of different methods for preparation of graft materials on the biological properties of tendon allograft.

METHODS: Forty-eight healthy male Leghorns were randomly divided into three groups: vitrification group, chemical extraction group, and control group. Unilateral superficial and deep flexor tendon of the third toe was subjected to vitrification, chemical extraction and no treatment in the three groups, respectively. A part of tendon was taken for biomechanical testing, and the other part was for allogeneic transplantation. After 1, 2, 3, 6 weeks, peripheral blood CD4⁺, CD8⁺ T lymphocytes were counted.

RESULTS AND CONCLUSION: Vitrification could partially retain the original tendon cells, but the chemical extraction method could not. Tensile strength for tendon rupture, tensile fracture power and tensile elongation at break were not statistically significant among three groups (P > 0.05). At the end of 1 and 2 weeks after transplantation, CD4⁺, CD8⁺, CD4⁺/CD8⁺ difference was significant among the three groups (P < 0.05); at the end of 3 and 6 weeks after transplantation, CD4⁺, CD8⁺, CD4⁺/CD8⁺ were significantly less in the vitrification and chemical extraction groups than the control group (P < 0.05), but no difference was found between the vitrification group and chemical extraction group (P > 0.05). These findings indicate that the vitrification and chemical extraction methods can significantly reduce immunogenicity of the tendon based on effective retention of biomechanical properties of the tendon.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, biomechanics, transplantation

CLC Number:

R318

Zhang Hong-xing, Liu Geng, Qiu Wu-an. Impact of different preparation methods for graft materials on biological properties of allogeneic tendon[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(39): 6348-6352.

Figures/Tables 4

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