1-Ethy1-3-(3-dimethylaminopropy1)carbodiimide hydrochloride cross-linking xenogeneic porcine acellular tendon for repairing Achilles tendon injury

doi:10.3969/j.issn.2095-4344.2013.12.009

Abstract

Abstract:

BACKGROUND: Heterogeneous tendon is an ideal method to repair tendon defects, but the post-transplantation rejection limits the application of heterogeneous tendons.
OBJECTIVE: To investigate the effect of transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular tendon of Banna inbred-lines miniature pig for repairing the rabbit Achilles tendon defects.
METHODS: Lateral resection of bilateral Achilles tendons from 40 Japanese rabbits was performed, and then the tendons were divided into experimental group and control group. The experimental group was repaired with transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular treated tendon of Banna mini-pig inbred-lines, and the control group was repaired with autologous tendon graft.
RESULTS AND CONCLUSION: Histological observation showed that the cells in the new tissue of two groups were mainly the mononuclear fibroblast and fibrocytes, and the cells were oval or circular fibroblasts at 1-4 weeks after operation; new collagen formation could be seen around the cells in the cell aggregation area, and the collagen represented disorderly; focal cord-like mature collagen was distributed in island shape and formed the so-called “collagen island”; at 12 weeks after operation, the cells were increasingly elongated and became spindle-shaped and long strip fibroblasts. Laboratory testing showed that there were no significant differences in white blood cells, C-reactive protein levels, hydroxyproline content and tensile strength between two groups. The results show that 1-ethy1-3-(3-dimethylaminopropy1) carbodiimide hydrochloride cross-linking acellular tendon from Banna mini-pig inbred-lines can repair rabbit Achilles tendon defects successfully with good histocompatibility, less transplant rejection and strong biomechanical performance.

Key words: biomaterials, material biocompatibility, tendon, carbodiimide hydrochloride, acellular, pig, xenogeneic transplantation, hydroxyproline, Achilles tendon injury, other grants-supported paper, biomaterials photographs- containing paper

CLC Number:

R318

Jin Xian-hui, Gao Chun-guang, Cui Sheng-jie, Liu Gui-hua, Yang Jian-bo, Lin Yue-qiu. 1-Ethy1-3-(3-dimethylaminopropy1)carbodiimide hydrochloride cross-linking xenogeneic porcine acellular tendon for repairing Achilles tendon injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(12): 2153-2158.

Figures/Tables 5

2.1 实验动物数量分析 40只兔均进入结果分析。 2.2 两组移植肌腱苏木精-伊红染色和Movat染色组织学光镜观察结果术后1周：实验组在光镜下均见到密度极高的椭圆形或圆形成纤维细胞，细胞间可见到胶原纤维，虽然这些胶原的走向较紊乱，但胶原整体构架尚完整，较致密，尚能分辨出其主要走向与跟腱纵轴平行，同时还可见到血管，见图1A。对照组胶原纤维纵行紧密排列，长梭形肌腱细胞位于纤维束间，见图1B。术后4周：两组均可见到大量椭圆形或圆形成纤维细胞聚集成团，细胞聚集区的细胞周围有新生胶原形成，这些胶原的走向较紊乱；局灶性条索状成熟胶原呈岛状分布，形成所谓“胶原岛”。在部分区域这些胶原条索形成的“胶原岛”分布较紧密，另一些区域“胶原岛”分布较疏散。“胶原岛”内胶原的走向与跟腱纵轴平行。整体胶原构架较疏松，出现一些“空白”的无组织区，见图1C，D。术后8周：两组出现岛状分布的“胶原岛”连接成带，这些条带与以细胞聚集和不成熟胶原为特征的区域(称其为不成熟胶原区) 间隔交错排列，有些索带贯穿整个标本，胶原走向与跟腱纵轴平行。“空白”无组织区面积缩小。不成熟胶原区内细胞以椭圆形或圆形为主，但出现梭形的纤维细胞。细胞周围新生胶原的走向虽仍略显紊乱,但仍能分辨出大致的走向与跟腱纵轴平行，见图1E，F。术后12周：两组均出现胶原条带连接成片，因而标本的大部分为成熟的胶原，胶原纤维的走向与跟腱纵轴平行，其中的细胞主要是梭形的纤维细胞。不成熟胶原区明显缩小，形成散在分布的局灶性区域，这些区域内细胞聚集，但细胞数量相对减少，细胞以椭圆形或圆形为主，见图1G，H。 Movat stain五套色染法成熟胶原纤维和新生胶原纤维，见图2。"

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