Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (36): 5805-5812.doi: 10.3969/j.issn.2095-4344.2014.36.012
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Zhang Hao, Qi Hai, Li Yuan-ming, Chen Jia-hua
Revised:
2014-06-03
Online:
2014-08-30
Published:
2014-08-30
Contact:
Qi Hai, Chief physician, Professor, Doctoral supervisor, Department of Cardiothoracic Surgery, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
About author:
Zhang Hao, Studying for master’s degree, Department of Cardiothoracic Surgery, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
CLC Number:
Zhang Hao, Qi Hai, Li Yuan-ming, Chen Jia-hua. Harmine has no lung-protective effects in a canine model of lung ischemia-reperfusion injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(36): 5805-5812.
2.1 肺组织及血液中白细胞介素17、肿瘤坏死因子α、内皮素1水平分析 实验组(低钾右旋糖苷+去氢骆驼蓬碱保护液灌洗组)与对照组(低钾右旋糖苷保护液灌洗组)中肺组织及血液的白细胞介素17、肿瘤坏死因子α、内皮素1水平进行比较,经方差齐性检验,方差齐(P > 0.05),采用两样本t 检验。经分析,两组白细胞介素17、肿瘤坏死因子α、内皮素1水平差异均无显著性意义(P > 0.05,表1)。从实际测得数据均值来看,再灌注2 h时,实验组中左肺组织及血液的白细胞介素17、肿瘤坏死因子α及血液中的内皮素1水平较对照组有所降低,说明肺损伤程度较对照组有所减轻;再灌注4 h时,实验组中左肺组织及血液的白细胞介素17、肿瘤坏死因子α、内皮素1水平较对照组有所升高,说明肺损伤程度较对照组有所加重。"
2.2 再灌注4 h后肺组织内病理指标分析 实验组与对照组中再灌注4 h的肺泡损伤指数、血管壁水肿程度、支气管肺泡灌洗液(BALF)中性粒细胞及淋巴细胞数目进行比较。经方差齐性检验,两组肺泡损伤指数进行比较方差不齐 (P =0.013),采用t' 检验,其余采用两样本t 检验。经分析,两组中的肺泡损伤指数、血管壁水肿程度、支气管肺泡灌洗液中性粒细胞及淋巴细胞数目差异均无显著性意义(P > 0.05,表2)。从实际测得数据均值来看,实验组中的肺泡损伤指数、灌洗液中性粒细胞数目较对照组有所降低,说明肺损伤程度有所减轻;而血管壁水肿程度、灌洗液淋巴细胞数目较对照组有所升高,说明肺损伤程度有所加重。"
2.4 肺动脉压力分析 采用单因素方差分析,两组的主肺动脉压、左肺动脉压及右肺动脉压差异均无显著性意义(P > 0.05,表3)。从图2,3可以看出,实验组的左肺动脉压均值在开放循环和开放后30 min最高,随后出现下降,在开放后150 min出现回升,之后缓慢下降后维持在26 mm Hg左右;对照组左肺动脉压均值在开放循环后出现缓慢上升,在开放后120 min达到峰值,随后出现缓慢下降后维持在30 mm Hg左右。从实际测得的数据均值来看,实验组的主肺动脉压、左肺动脉压及右肺动脉压均值低于对照组,说明实验组肺循环阻力较对照组有所降低,肺血管收缩程度较对照组有所减轻,机制可能与血管舒张因子的生成、释放有关,有待于进一步研究证实。单从本实验肺动脉压力进行分析,实验组的肺保护程度比对照组好。"
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