Low-frequency magnetic fields promote neovascularization in a rabbit model of atherosclerosis combined with limb ischemia

doi:10.3969/j.issn.2095-4344.2014.27.018

Abstract

Abstract:

BACKGROUND: Studies on basic research of magnetic treatment of limb ischemic disease are not much, because poor compliance of animals and the stability of the magnetic field strength are difficult to control, resulting in big experimental error and decreased credibility of the results. For this kind of problem, experimental study on low-frequency electromagnetic magnetic cages for treatment of ischemic limbs was conducted, thus overcoming the two major issues of poor compliance of animals and difficult control of the stability of magnetic field strength.
OBJECTIVE: To investigate the effects of self-made low-frequency magnetic fields of rabbit cages on neovascular growth-promoting factor of rabbits with limb ischemia.
METHODS: A total of 96 rabbit models of atherosclerosis were constructed, numbered and randomly divided into ischemia group and non-ischemia group (12 treatment combination in each group). Experiments in each group were performed four times according to the requirement of factorial design. Electromagnetic field intensity factor A (0, 3, 6, 12 mT) and the time factor B (3, 5, 7 days) were set.
RESULTS AND CONCLUSION: Low-frequency magnetic field could apparently promote hypoxia inducible factor-1α, vascular endothelial growth factor and CD34 expression in ischemic limb of rabbits. Electromagnetic field intensity factor A was a key factor for contributing to the expression of hypoxia inducible factor-1α, vascular endothelial growth factor and CD34, and the time factor B was secondary factor. Low-frequency magnetic field also promoted hypoxia inducible factor-1α expression in non-ischemia limb, but did not promote vascular endothelial growth factor and CD34 expression. Thus, the expression of vascular endothelial growth factor and CD34 was regulated by hypoxia inducible factor-1α, as well as other factors, in the ischemic state.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: electromagnetic fields, atherosclerosis, ischemia, muscle, striated, hypoxia-inducible factor 1, alpha subunit, vascular endothelial growth factors

CLC Number:

R318

Zhang Jian-yong, Peng Hui, Meng De-kai, Chen Hui, Zhang Yun-qiang, Yu Hua-long. Low-frequency magnetic fields promote neovascularization in a rabbit model of atherosclerosis combined with limb ischemia[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(27): 4375-4382.

Figures/Tables 5

References

[1]Criqui MH, Fronek A, Barrett-Connor E,et al.The prevalence of peripheral arterial disease in a defined population. Circulation. 1985;71(3):510-515.
[2]Newman AB, Sutton-Tyrrell K, Vogt MT,et al.Morbidity and mortality in hypertensive adults with a low ankle/arm blood pressure index.JAMA. 1993;270(4):487-489.
[3]Klinkert P, Schepers A, Burger DH,et al.Vein versus polytetrafluoroethylene in above-knee femoropopliteal bypass grafting: five-year results of a randomized controlled trial.J Vasc Surg. 2003;37(1):149-155.
[4]van Weel V, van Tongeren RB, van Hinsbergh VW,et al.Vascular growth in ischemic limbs: a review of mechanisms and possible therapeutic stimulation.Ann Vasc Surg. 2008;22(4):582-597.
[5]Kalka C, Baumgartner I.Gene and stem cell therapy in peripheral arterial occlusive disease.Vasc Med. 2008;13(2):157-172.
[6]Tateishi-Yuyama E, Matsubara H, Murohara T,et al.Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial.Lancet. 2002;360(9331):427-435.
[7]Hoshino J, Ubara Y, Hara S,et al.Quality of life improvement and long-term effects of peripheral blood mononuclear cell transplantation for severe arteriosclerosis obliterans in diabetic patients on dialysis.Circ J. 2007;71(8):1193-1198.
[8]刘傥,张湘生,雷霆,等.带部分松质骨小牛皮质骨支架复合兔骨髓间充质干细胞植入兔体内血管内皮生长因子的表达[J]. 中国组织工程研究与临床康复,2010,14(34):6287-6292.
[9]孙英华,王莉,焦兆德.血管内皮生长因子对免异体重建后交叉韧带血管再生的影响[J].中国组织下程研究与临床康复,2008, 12(42):8225-8229.
[10]樊国华.干细胞移植干预下肢缺血性疾病[J].中国组织工程研究与临床康复,2007,11(50):10154-10156.
[11]王三明,赵刚,胡世雄,等.血管内皮生长因子转染内皮祖细胞治疗大鼠缺血后肢的研究[J]. 中华实验外科杂志,2009,26(12):1625-1627.
[12]马丽,黄鲁刚,林苹.体外诱导脂肪干细胞向内皮细胞及成血管的分化[J].中国组织工程研究,2013,17(10):1730-1735.
[13]高延明,张路. 骨髓间充质干细胞移植治疗糖尿病足过程中血管内皮生长因子的表达[J].中国组织工程研究,2013,17(40):7169-7174.
[14]李华,高建华,颜玲.成人外周血单个核细胞体外培养过程中分化为早及晚期内皮祖细胞生物学表征[J].中国组织工程研究与临床康复,2007,11(24):4682-4685.
[15]Yen-Patton GP, Patton WF, Beer DM, et al.Endothelial cell response to pulsed electromagnetic fields: stimulation of growth rate and angiogenesis in vitro.J Cell Physiol. 1988;134(1):37-46.
[16]Matsumoto H, Kira K, Kondoh K,et al.Effects of alternately aligned static micromagnetic fields on intravascular endothelial lining.Angiology. 1992;43(9):757-764.
[17]李飞,贾国良,张荣庆,等.弱恒磁场对人脐静脉及兔主动脉内皮细胞增殖的影响[J].中国病理生理杂志,2003,19(6): 772,777.
[18]张杰,陆洪英,金成文,等.恒磁场对人脐静脉内皮细胞增殖、凋亡及分泌功能的影响[J].中华物理医学与康复杂志,2011,33(10): 742-745.
[19]郭影,李飞,郭文怡,等.低频脉冲磁场对大鼠骨髓源内皮祖细胞增殖和体外血管化的影响[J].岭南心血管病杂志,2009,15(2):131-135.
[20]冯玉,白文芳,许伟成,等. 低频电磁场促进骨髓间充质干细胞移植修复大鼠脊髓损伤的实验研究[J].中国组织工程研究,2013, 17(32):5819-5826.
[21]张建勇,陈辉,刘小庆,等.磁性饲养笼的研制[J].新疆中医药,2013, 31(2):5-7.
[22]张建勇,彭辉,陈辉,等. 磁性饲养笼低频电磁场对家兔缺血肢体新生血管形成的实验研究[J].新疆医科大学学报,2013,36(8): 1086-1090.
[23]张建勇,彭辉,陈辉.家兔髂总动脉结扎后不同时间缺血肢体病理变化特征研究[J].新疆医科大学学报,2013,36(9):1268-1271.
[24]谢作煊,李梅兰,何文芳.家兔和大鼠实验性动脉粥样硬化的比较研究[J].解剖学研究,2000,22(2):98-101.
[25]Babuccu O, Peksoy I, Hosnuter M,et al.Evaluation by scintigraphy of hindlimb ischemia in a rat model.J Reconstr Microsurg. 2004;20(5):405-410.
[26]张刚,谭军,李高峰.兔耳瘢痕成熟过程中血管内皮生长因子与缺氧诱导因子1α的表达[J].中国组织工程研究与临床康复,2011, 15(7): 1191-1194.
[27]黄烨,陈登峰,袁璐,等.乳腺肿块微血管密度与血氧近红外光参数的实验研究[J].肿瘤防治研究,2007,34(1):51-53.
[28]肖建伟,胡芸,李翔,等.早期动脉硬化斑块的MSCT动物实验研究及病理学对照[J]. 实用放射学杂志,2011,27(12):1925-1927.
[29]张桂运,王硕,王岩,等.动脉粥样硬化兔不同程度血液动力性脑缺血模型的建立[J]. 同济大学学报(医学版),2011,32(2):15-18.
[30]邵娜,陈文强,李大庆,等.口服雷帕霉素抑制支架内再狭窄的血管内超声的实验研究[J]. 山东大学学报(医学版),2011,49(6):59-63.
[31]薛峰,杜大勇,李运田.瑞舒伐他汀对兔动脉粥样硬化斑块内CD147的表达及斑块稳定性的影响[J].山西医科大学学报, 2013, 44(4):258-262 .
[32]李运田,柳杨,杜大勇,等.白藜芦醇对兔动脉粥样硬化基质金属蛋白酶表达的影响[J].军医进修学院学报,2010,31(4): 356-357,366.
[33]沈伟,施海明,罗心平,等.兔实验性动脉粥样硬化和急性心肌梗死双模型的建立[J].中国实验动物学报,2010,18(4):318-321.
[34]方伟,郭曙光,庞荣清,等.兔后肢缺血模型的制作[J].云南医药, 2008,29(2):126-129.
[35]孙晓峰,所剑,王琦,等.家兔后肢缺血模型制作[J].中国实验诊断学, 2009,13(1):52-53.
[36]Pu LQ, Jackson S, Lachapelle KJ,et al.A persistent hindlimb ischemia model in the rabbit.J Invest Surg. 1994;7(1):49-60.
[37]刘昭鸿,孙崇新,张文道,等.磁光复合疗法的临床研究[J].生物医学工程学杂志,2003, 20(2): 305-307.
[38]Pugh CW, Ratcliffe PJ.Regulation of angiogenesis by hypoxia: role of the HIF system.Nat Med. 2003;9(6):677-684.
[39]Mazure NM, Brahimi-Horn MC, Pouysségur J.Protein kinases and the hypoxia-inducible factor-1, two switches in angiogenesis. Curr Pharm Des. 2003;9(7):531-541.
[40]张刚,谭军,李高峰,等.兔耳瘢痕成熟过程中血管内皮生长因子与缺氧诱导因子1α的表达[J].中国组织工程研究与临床康复, 2011,15(7):1191-1194.
[41]Kagiwada H, Yashiki T, Ohshima A,et al.Human mesenchymal stem cells as a stable source of VEGF-producing cells.J Tissue Eng Regen Med. 2008;2(4): 184-189.
[42]Bekhite MM, Finkensieper A, Abou-Zaid FA, et al.Static electromagnetic fields induce vasculogenesis and chondro-osteogenesis of mouse embryonic stem cells by reactive oxygen species-mediated up-regulation of vascular endothelial growth factor.Stem Cells Dev. 2010;19(5): 731-743.
[43]孙晓峰,所剑,王琦. HIF-1α与VEGF在动脉粥样硬化闭塞症患者缺血下肢血管及肌组织中的表达及意义[J].吉林大学学报:医学版,2009,35(2):337-340.
[44]董智慧,魏征,符伟国,等.纯化自体外周血CD34+细胞移植治疗下肢重度缺血[J].中华普通外科杂志,2011,26(3):184-187.
[45]Tateishi-Yuyama E, Matsubara H, Murohara T,et al.Therapeutic angiogenesis for patients with limb ischaemia by autologous transplantation of bone-marrow cells: a pilot study and a randomised controlled trial.Lancet. 2002;360 (9331):427-435.
[46]Horie T, Onodera R, Akamastu M,et al.Long-term clinical outcomes for patients with lower limb ischemia implanted with G-CSF-mobilized autologous peripheral blood mononuclear cells.Atherosclerosis. 2010;208(2):461-466.
[47]师天雄,缪健航,张明光,等.自体外周血干细胞移植治疗患者下肢动脉缺血性疾病的随访研究[J].中华普通外科学文献:电子版, 2008,2(5):371-373.
[48]Asahara T, Masuda H, Takahashi T,et al.Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization.Circ Res. 1999;85(3):221-228.
[49]Tepper OM, Galiano RD, Kalka C, et al.Endothelial progenitor cells: the promise of vascular stem cells for plastic surgery. Plast Reconstr Surg. 2003;111(2):846-854.