Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2477-2480.doi: 10.3969/j.issn.1673-8225.2012.14.002

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In vitro culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells   

Li Song, Guo Yan-wei, Fang Dian-ji   

  1. Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Jiamusi University Hospital, Jiamusi  154000, Heilongjiang Province, China
  • Received:2011-12-08 Revised:2012-03-06 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Fang Dian-ji, M.D., Master’s supervisor, Associate chief physician, Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Jiamusi University Hospital, Jiamusi 154000, Heilongjiang Province, China dianjifang@yahoo.com.cn
  • About author:Li Song★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Stomatology Hospital of Jiamusi University Hospital, Jiamusi 154000, Heilongjiang Province, China yitao1028@hotmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81170935*

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Abstract:

BACKGROUND: Previous studies have demonstrated that bone marrow mesenchymal stem cells are harvested primarily from femur or ilium, but there have been few reports describing mandible-derived bone marrow mesenchymal stem cells.
OBJECTIVE: To investigate in vitro isolation, culture and biological characteristics of rabbit mandible-derived bone marrow mesenchymal stem cells.
METHODS: Bone marrow mesenchymal stem cells were harvested from rabbit mandible and isolated by density gradient centrifugation. Cells were adherently cultured in vitro, and passage 2 or 3 bone marrow mesenchymal stem cells were used for detection. Cell proliferation was detected by MTT assay. Fibroblast colony forming units were detected by examination of colony formation. The potential of bone marrow mesenchymal stem cells differentiation into osteoblasts and adipocytes was detected.
RESULTS AND CONCLUSION: MTT assay showed that the cell growth curves of mandible-derived bone marrow mesenchymal stem cells exhibited incubation period, logarithmic phase and platform period. Colony-formation assays showed that cells exhibited the appearance of fibroblast colony forming units. After Aalizarin red and oil red O staining, there were mineralized nodules and lipid drops. These findings suggest that rabbit mandible-derived bone marrow mesenchymal stem cells show strong self-renewal and proliferative capacities and exhibit the potential of multi-directional differentiation.

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