Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2471-2476.doi: 10.3969/j.issn.1673-8225.2012.14.001

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Induced differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by four inductors   

Liu Bo-wu, Lü An-lin, Huang Wei, Hou Jing, Hou Zhao-lei, Hou Hong, Da Jing, Yang Na   

  1. Department of Cardiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an   710032, Shaanxi Province, China
  • Received:2011-12-05 Revised:2012-01-12 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Lü An-lin, Associate chief physician, Associate professor, Department of Cardiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China lvanlin@fmmu.edu.cn
  • About author:Liu Bo-wu★, Studying for master’s degree, Department of Cardiology, Xijing Hospital, Fourth Military Medical University of Chinese PLA, Xi’an 710032, Shaanxi Province, China lbw_113@hotmail.com

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Abstract:

BACKGROUND: It has been rarely reported that different inductors are used to induce bone marrow mesenchymal stem cells to differentiate into cardiomyocyte-like cells. 
OBJECTIVE: To investigate the effects of 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, pifithrin-α on induced differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells. 
METHODS: Passage 3 rat bone marrow mesenchymal stem cells were divided into five groups: control, 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, pifithrin-α. Surface antigen expression of bone marrow mesenchymal stem cells was identified by flow cytometry. After 4 weeks of induction, Cx43 and troponin Ⅰ expression was detected by western blot method and calcium transient capability of induced bone marrow mesenchymal stem cells was detected by laser confocal scanning microscopy with flou3/AM.
RESULTS AND CONCLUSION: Cell surface antigen CD29, CD44, CD45 positive rate was 89.1%, 90.4%, 1.9% respectively. Troponin Ⅰ expression appeared in each group and the expression was significantly higher in the pifithrin-α group (P < 0.05), as well as significantly lower in the bone morphogenetic protein 2 group (P < 0.05), compared with the remaining groups. Cx 43 expression appeared in each group, and the expression was significantly lower in the control group (P < 0.05) compared with the remaining groups. In the four experimental groups, Cx43 expression was highest in the pifithrin-α group and lowest in the angiotensin Ⅱ group (P < 0.05). Calcium transient capability determined by fluorescence intensity was arranged as follows: angiotensin Ⅱ group > pifithrin-α group > 5-azacytidine group > bone morphogenetic protein 2 group > control group (P < 0.01). These findings suggest that after induced by 5-azacytidine, angiotensin Ⅱ, bone morphogenetic protein 2, and pifithrin-α, bone marrow mesenchymal stem cells differentiated towards cardiomyocyte-like cells and expressed myocardium-specific protein. Different inductors exert different effects on differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells which occurs because of different pathways.

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