Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (41): 7241-7247.doi: 10.3969/j.issn.2095-4344.2013.41.009
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Aikeremujiang•Muheremu1, 2, Cao Peng1, Wu Zhong-yan1, Wang Xin-ling1, Jia Min1
Received:
2013-07-24
Revised:
2013-08-05
Online:
2013-10-08
Published:
2013-11-01
Contact:
Wu Zhong-yan, Chief physician, Department of Orthopedics, the Fifth Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
wzyfah@163.com
About author:
Aikeremujiang?Muheremu☆, Studying for doctorate, Physician, Department of Orthopedics, the Fifth Hospital Affiliated to Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China; Beijing Jishuitan Hospital of Tsinghua University, Beijing 100035, China
taklimakan321@hotmail.com
Supported by:
Scientific Research and Innovation Fund of Xinjiang Medical University, No. XJC2012122*, XJC2012117*, XJC2012123*
CLC Number:
Aikeremujiang•Muheremu, Cao Peng, Wu Zhong-yan, Wang Xin-ling, Jia Min. Selective regeneration of rat femoral nerve after crush injury and freeze injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(41): 7241-7247.
各组大鼠分别于造模后2,3,6,12周根据BBB评分量表进行行为学评分。 夹伤组与冰冻损伤组大鼠术侧后腿活动范围均缩小,伸腿功能均受限。 夹伤组与冰冻损伤组大鼠后肢BBB评分造模后随时间延长而增加,夹伤组与冰冻损伤组的BBB评分显著低于对照组(P < 0.05),夹伤组与冰冻损伤组之间各时间点BBB评分差异无显著性意义(P > 0.05)。 2.3 电生理检测 术侧电极刺激吻合口近端神经,对侧刺激相对应位置。于股四头肌处可记录到运动诱发电位。 夹伤组潜伏期为(1.78±0.33) ms,波幅(4.21± 0.87) mV,对照组潜伏期(1.66±0.25) ms,波幅(5.83±1.20) mV;冰冻损伤组潜伏期(1.80±0.41) ms,波幅(4.81±1.52) mV。 与对照组大鼠正常神经比较,夹伤组与冰冻损伤组的潜伏期延长(P < 0.05),波幅降低(P < 0.05),但夹伤组与冰冻损伤组大鼠之间差异无显著性意义(P > 0.05)。 2.4 逆行示踪 脊髓冰冻切片后,置于荧光显微镜下,在不同颜色的激发光下,观察到对照组中脊髓前角仅有红色标记的运动神经元,见图1;而夹伤组与冰冻损伤组可同时观察到红色、蓝色以及紫色染的神经元,见图2及表2。"
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