Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (39): 5825-5833.doi: 10.3969/j.issn.2095-4344.2016.39.008

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Experimental animal models of intervertebral fusion-induced adjacent segment degeneration

Liu Ying-jie1, Peng Jun1, Liu Xiao-kang2, Zhao Cheng3, Yang Er-zhu4, Xu Jian-guang5   

  1. 1Department of Orthopedics, Eastern Branch, Shanghai Sixth People’s Hospital, Shanghai 201306, China; 2Department of Orthopedics, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450003, Henan Province, China; 3Department of Orthopedics, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China; 4Department of Orthopedics, 5Department of Spine Surgery, Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Revised:2016-07-04 Online:2016-09-23 Published:2016-09-23
  • Contact: Xu Jian-guang, Doctoral supervisor, Department of Spine Surgery, Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • About author:Liu Ying-jie, Master, Attending physician, Department of Orthopedics, Eastern Branch, Shanghai Sixth People’s Hospital, Shanghai 201306, China

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Abstract:

BACKGROUND: Upright rat intervertebral disc degeneration model can prove that intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration, but cannot affirm that spinal non-fusion technology has more advantages.

OBJECTIVE: Base on the affirmed intervertebral disc degeneration models of Beagle dog, we performed intervertebral fusion to affirm whether or not the intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration.
METHODS: Twelve Beagle dogs were randomly divided into two groups. The control group animals were stabbed percutaneous to injure L5/6 disc. The experimental group animals were stabbed percutaneous to injur L5/6 disc. One month later, we made L4/5 intervertebral fusion. At 3 and 6 months postoperatively, lumbar MRI was conducted. L5/6 discs were harvested and subjected to histological observation and PCR.
RESULTS AND CONCLUSION: (1) MRI: in the experimental group, 3 and 6 months after fusion, different degrees of intervertebral disk herniation appeared. In the control group, no obvious intervertebral disk herniation appeared. (2) Histological observation: 6 months after fusion, in the experimental group, intervertebral disc annulus and nucleus pulposus were disordered; there was a gap in the fold, and the number of nucleus pulposus cells decreased. In the control group, nucleus pulposus and anulus fibrosus boundary was clear; collagen was loose and arranged regularly. Cell number was more. Anulus fibrosus was close to normal. Collagen fibers were regular and dense. In the experimental group, 3 and 6 months after fusion, type II collagen-positive cells were less than the control group (P < 0.05). Moreover, type II collagen-positive cells were less at 6 months than at 3 months in the experimental group (P < 0.05). (3) PCR: Bone morphogenetic protein 15 and tissue inhibitor of matrix metalloproteinase 1 gene expression was higher in the experimental group than in the control group at 3 and 6 months after fusion (P < 0.05). Bone morphogenetic protein 15 and tissue inhibitor of matrix metalloproteinase 1 gene expression was higher in the experimental group at 6 months than that at 3 months (P < 0.05). (4) These results suggest that intervertebral fusion can aggravate the adjacent-segment intervertebral disc degeneration.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

Key words: Spinal Fusion, Intervertebral Disk Degeneration, Models, Animal, Tissue Engineering

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