Identification of bone marrow stem cells

doi:10.3969/j.issn.2095-4344.2012.49.032

Abstract

Abstract:

BACKGROUND: Single specific stem cells are always used in the scientific research. An identification technique is required to confirm the isolated cells.
OBJECTIVE: To introduce the identification technique used to confirm the isolated bone marrow mesecnhymal stem cells and bone marrow hemopoietic stemcells. METHODS: A computer-based online retrieval was performed to search papers describing identification of bone marrow mesenchymal stem cells and bone marrow hemopoietic stem cells published during 1998-01/2010-12 in the PubMed database and Weipu database. The key words are bone marrow stem cell, bone marrow hematopoietic stem cells, identification in Chinese and English. After excluding repetitive studies, 31 papers were included in the final analysis.
RESULTS AND CONCLUSION: There has been no specific surface marker as a gold standard to identify bone marrow mesenchymal stem cells. Bone marrow mesenchymal stem cells can be detected from cell morphology and culture feature, molecular marker, multi-differentiation potential. Generally, the cells that have a fibrocyte-like morphology, can be adhered, divided, and proliferated, and express CD44 and CD29 rather than CD34 and CD45, are considered as bone marrow mesenchymal stem cells. At present, the real phenotype for bone marrow hemopoietic stem cells has not been determined. Spleen clone formation, in vitro clone formation and flow cytometry are the primarily used methods to detect bone marrow hemopoietic stem cells.

CLC Number:

R318

Xu Mei-mei, Gao Fu-lai1, Han Ming-zi, Zheng Yue, Jin Shi-zhu, Lü Chun-yan. Identification of bone marrow stem cells[J]. Chinese Journal of Tissue Engineering Research, 2012, 16(49): 9301-9305.

Figures/Tables 1

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