Effects of resveratrol on albumin-induced pyroptosis-related proteins in HK-2 cells

doi:10.12307/2023.094

Abstract

Abstract: BACKGROUND: Resveratrol exerts protective effects against renal diseases, while its protective mechanism against inflammatory injury of renal tubular epithelial cells induced by albumin is still unclear.
OBJECTIVE: To explore the effect of resveratrol on pyroptosis-related protein expression in HK-2 cells.
METHODS: Albumin was used to induce inflammatory damage in HK-2 cells. Resveratrol and sirtuins (SIRT1) inhibitor EX527 were used as pretreatments. MTT assay was used to assess cell viability. Western blot was applied to test the protein expression of SIRT1, NOD-like receptor protein 3 (NLRP3), caspase-1 and apoptosis-associated speck-like protein (ASC). Immunofluorescence double staining was used to observe the co-localization of NLRP3 and ASC. Hoechst33342/PI staining was used to observe pyrolysis. Levels of interleukin-1β and interleukin-18 were tested by using ELISA.
RESULTS AND CONCLUSION: Albumin could significantly decrease cell viability, elevate the expressions of interleukin-1β, interleukin-18 and NLRP3 inflammasome (P < 0.01), and increase pyroptosis in HK-2 cells. However, resveratrol significantly upregulated the protein expression of SIRT1 in HK-2 cells (P < 0.01), downregulated the expression of NLRP3, ASC and caspase-1 (P < 0.05) and the levels of interleukin-1β and interleukin-18 (P < 0.01), and attenuated albumin-induced pyroptosis. SIRT1 inhibitor could remarkably increase the expression of NLRP3 inflammasome and increase pyrolysis. To conclude, resveratrol modulates HK-2 cell pyroptosis-related proteins to ameliorate albumin-induced inflammatory injury in renal tubular epithelial cells.

Key words: resveratrol, renal tubular epithelial cell, NLRP3 inflammasome, pyroptosis, albumin

CLC Number:

Li Fang, Cao Jianmin, Zhai Pengfei, Wang Jianshe, Hong Da. Effects of resveratrol on albumin-induced pyroptosis-related proteins in HK-2 cells[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(14): 2164-2169.

Figures/Tables 7

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