A single bout of exhaustive exercise induces renal NOD-like receptor protein 3 inflammasome expression in rats

doi:10.12307/2022.032

Abstract

Abstract: BACKGROUND: Exhaustive exercise leads to multiple redistribution of blood oxygen in the body, induces a strong inflammatory response, enhances the level of systemic inflammation, and damages kidney function.
OBJECTIVE: To determine the effects of a single bout of exhaustive exercise on the expressions of renal NOD-like receptor protein 3 (NLRP3) inflammasome in rats and possible mechanism.
METHODS: Twenty male Sprague-Dawley rats aged 3 months were randomly divided into a control group and an exhaustive exercise group, with 10 rats in each group. In the exhaustive exercise, three-level incremental exercise load treadmill training was performed to establish a one-time exhaustive exercise experimental animal model. No modeling was performed in the control group. Immediately after training, blood sample was collected from each rat, and then the kidney tissue was removed for hematoxylin-eosin staining to observe the morphological changes of renal cells. Renal thioredoxin-interacting protein (TXNIP), serum blood urea nitrogen, creatinine, creatine kinase, malondialdehyde, cystatin C and urine kidney injury molecule-1 levels were detected. Western blot was used to detect renal SIRT1, NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC) 1, Caspase-1 and interleukin-1β protein expression. RT-qPCR was performed to detect serum and renal miR-155 and renal SIRT1 mRNA expression. Meanwhile, renal cell morphology was observed.
RESULTS AND CONCLUSION: Compared with the control group, the levels of serum and renal miR-155 were significantly increased in the exhaustive exercise group (P < 0.01, P < 0.05). Compared with the control group, the protein and mRNA expression of renal SIRT1 was significantly decreased (both P < 0.01), while the protein expression of renal TXNIP, NLRP3, ASC-1, Caspase-1 and interleukin-1β was significantly increased in the exhaustive exercise group (P < 0.05 or 0.01). Moreover, the levels of serum blood urea nitrogen, creatinine, creatine kinase, malondialdehyde, cystatin C and urine kidney injury molecule-1 were significantly increased in the exhaustive exercise group compared with the control group (P < 0.05 or 0.01), and renal pathologic changes were aggravated. The expression of SIRT1 was negatively related to the protein expression of TXNIP and NLRP3 (r=-0.962, P < 0.01; r=-0.977, P < 0.01). The expression of miR-155 was negatively related to the protein expression of SIRT1 (r=-0.989, P < 0.01), and positively related to the protein expression of TXNIP and NLRP3 (r=0.902, P < 0.01; r=0.968, P < 0.05). Therefore, a single bout of exhaustive exercise increases the levels of serum and renal miR-155, reduces the expression of renal SIRT1 protein and mRNA, increases the expression of renal TXNIP, activates NLRP3 inflammasome, triggers inflammatory response and then aggravates the renal pathological damages, thereby impairing renal function. This may be one of the mechanisms of kidney injury induced by a single bout of exhaustive exercise.

Key words: a single bout of exhaustive exercise, kidney, inflammatory injury, miR-155, SIRT1, NLRP3 inflammasome, rat

CLC Number:

Geng Yuanwen, Lin Qinqin, Li Ruoming, Tang Shaokai, Wang Baihui, Tian Zhenjun. A single bout of exhaustive exercise induces renal NOD-like receptor protein 3 inflammasome expression in rats[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(2): 190-196.

Figures/Tables 6

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