Canine adipose-derived mesenchymal stem cells and their exosomes in the repair of gentamicin-induced renal tubular epithelial cell injury

doi:10.3969/j.issn.2095-4344.2089

Abstract

Abstract:

BACKGROUND: Canine kidney injury is characterized by the apoptosis and necrosis of renal tubular epithelial cells. Recent developments in mesenchymal stem cells and their exosomes research have shown great promise for the treatment of kidney injury in humans, rats and mice, but little research has been done on dogs.

OBJECTIVE: To investigate the effects of canine adipose-derived mesenchymal stem cells and their exosomes on canine renal tubular epithelial cell injury induced by gentamicin in vitro.

METHODS: Canine renal tubular epithelial cells were treated by 5 mmol/L gentamicin sulfate. Subsequently, canine adipose-derived mesenchymal stem cells and their conditional medium and exosomes were co-cultured with damaged canine renal tubular epithelial cells respectively. After 24 and 48 hours, the cell proliferation activity of each group was measured by cell counting kit-8 method, and the apoptosis rate of each group was detected by flow cytometry. Finally, Q-PCR was used to further reveal the effects of canine adipose-derived mesenchymal stem cell exosomes on PCNA, Bcl-2 and Bax genes in these cells.

RESULTS AND CONCLUSION: Canine adipose-derived mesenchymal stem cells, their conditioned media, and exosomes could significantly promote proliferation and reduce apoptosis in damaged canine renal tubular epithelial cells (P < 0.05). Among them, canine adipose-derived mesenchymal stem cell exosomes worked best, which could significantly increase the expression of PCNA and Bcl-2 genes in damaged canine renal tubular epithelial cells (P < 0.05). These results suggest that canine adipose-derived mesenchymal stem cells can repair the canine renal tubular epithelial cell damage induced by gentamicin through their exosomes.

Key words:

canine, adipose-derived mesenchymal stem cells, exosomes, gentamicin, renal tubular epithelial cells, MDCK cells

CLC Number:

Lin Jiaying, Chen Shuyi, Chen Shengfeng, Wang Bingyun, Chen Zhisheng, Liu Canying, Bai Yinshan, Ji Huiqin, Xie Shiting. Canine adipose-derived mesenchymal stem cells and their exosomes in the repair of gentamicin-induced renal tubular epithelial cell injury [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(25): 3959-3965.

Figures/Tables 5

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