Salbutamol attenuates hyperoxia-induced acute lung injury in rats

doi:10.12307/2022.664

Abstract

Abstract: BACKGROUND: Hyperoxia-induced acute lung injury has attracted more and more attention in clinical practice. Studies have shown that salbutamol can reduce inflammation in rats and improve the activity of various antioxidants and microcirculation, thereby reducing pulmonary edema, and exerting a protective role in lung tissue.
OBJECTIVE: To investigate the effect of salbutamol on relieving hyperoxia-induced acute lung injury and its mechanism.
METHODS: Sprague-Dawley rats were divided into a control group, a model group in which lung injury model was prepared with high concentration oxygen at different time, and a treatment group given different dosages of salbutamol by gavage (0.025, 0.05, 0.1, 0.2, 0.4 mg/kg). The optimal high concentration oxygen (> 95%) treatment time and dosage of salbutamol were determined by measuring various biochemical indicators. Real-time quantitative PCR, western blot, and hematoxylin-eosin staining were then used to determine the mRNA and protein expressions in JNK/PI3K/Akt/NF-κB/ICAM-1 signaling pathway and the pathological changes of lung tissue in the model rats with lung injury given the optimal dose of salbutamol.
RESULTS AND CONCLUSION: The hyperoxia-induced acute lung injury model was successfully constructed and showed the best quality when treated with high concentration oxygen at 60 hours. The optimal dosage of salbutamol was 0.2 mg/kg. Compared with the control group, the biochemical indicators showed a significant increase or decrease in the model group (P < 0.05). The mRNA and protein expression levels of JNK/PI3K/Akt/NF-κB/ICAM-1 signaling pathway-related factors (JNK, PI3K, Akt, nuclear factor E2 related factor 2, nuclear factor κB, intercellular adhesion molecule 1, interleukin 8, tumor necrosis factor) were significantly up-regulated in the model group compared with the control group (P < 0.05). A significant regression was observed after treatment with salbutamol (P < 0.05). Besides, the model group showed severe pathological damage and inflammatory infiltration, and salbutamol could effectively alleviate these changes in the model group. To conclude, salbutamol can significantly improve the lung injury in rats with hypoxia-induced acute lung injury through JNK/PI3K/Akt/NF-κB/ICAM-1 signaling pathways.

Key words: salbutamol, improvement, hyperoxia-induced acute lung injury, signal pathway, mechanism

CLC Number:

Mei Hong, Qin Song, Li Kang, Chen Miao. Salbutamol attenuates hyperoxia-induced acute lung injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(23): 3657-3663.

Figures/Tables 5

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