Next-generation sequencing of identifying the human leukocyte antigen-A*24:353 and its gene frequency analysis

doi:10.12307/2021.012

Abstract

Abstract:

BACKGROUND: High resolution validation of human leukocyte antigen (HLA) is one of the essential step before hematopoietic stem cell transplantation. With the continuous development of the work and the number of people tested continues to increase, human leukocyte antigen new alleles are constantly emerging. Due to the late discovery of some new genes and the lack of relevant reports, the gene frequency cannot be accurately calculated. In the absence of relevant data, it is easy to misjudge the typing results. The result of mismatch affects the success rate of transplantation.

OBJECTIVE: An allele of HLA-A*24:353 was detected and confirmed in HLA, and its occurrence frequency was analyzed.
METHODS: Polymerase chain reaction-sequencing-based typing method was used to detect five loci of HLA- A, B, C, DRB1, and DQB1 in 543 clinical transplantation matching recipients from September 2019 to May 2020. Next-generation sequencing was used to determine the full-length sequence of HLA-A*24:02/24:353 ambiguous results to obtain high resolution typing results of HLA and calculate the occurrence frequency of HLA-a *24:353 gene.
RESULTS AND CONCLUSION: The ambiguous results of HLA-A*24:02/24:353 were detected in 146 cases. After further confirmatory tests using next-generation sequencing, the results of HLA-A*24:353 gene were detected in 5 cases (3.42%) in A*24 group and 0.92% in 543 cases. The results suggest that HLA-A*24:353 may not be rare allele in China. When HLA-A*24:02/24:353 ambiguous results appear in clinical transplantation matching recipients for high resolution typing, further confirmation is needed to increase the accuracy of typing results and improve the success rate of transplantation matching.

Key words: hematopoietic stem cells, transplantation, human leukocyte antigen, allele, frequency, transplantation matching, blood

CLC Number:

He Liumei, Chen Hao, Zhong Yanping, Quan Zhanrou, Zou Hongyan. Next-generation sequencing of identifying the human leukocyte antigen-A*24:353 and its gene frequency analysis[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(25): 4009-4012.

Figures/Tables 4

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