Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (32): 5179-5185.doi: 10.3969/j.issn.2095-4344.2868
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Cao Qingjun, Yang Fenghua, Wang Hua
Department of Pediatric Neurology in Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Received:
2018-12-14
Revised:
2018-12-19
Accepted:
2019-09-07
Online:
2020-11-18
Published:
2020-09-25
Contact:
Wang Hua, MD, Professor, Doctoral supervisor, Chief physician, Department of Pediatric Neurology in Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
About author:
Cao Qingjun, MD, Department of Pediatric Neurology in Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China
Supported by:
the Science and Technology Plan of Liaoning Province, No. 2014225007
CLC Number:
Cao Qingjun, Yang Fenghua, Wang Hua. Hippocampal astrocytes in juvenile rats with persistent epilepsy: the role of cannabinoid receptor type 2 in regulating MAPK pathway[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(32): 5179-5185.
1.4.5 Real-time PCR检测大鼠脑海马组织中GFAP mRNA的表达 取出-80 ℃冷冻保存的幼年大鼠海马组织,在EP管中加入50 mg组织与1 mL Trizol,剪碎脑组织并用5 mL注射器抽打,提取细胞沉淀中的总RNA,紫外分光光度计测定总RNA的浓度和纯度,按Takara(RR047A)反转录试剂盒条件配20 μL体系进行反转录,取出标本,按Takara(RR047A)扩增试剂盒说明加入引物(Takara公司设计合成,见表1)及试剂配20 μL体系进行PCR扩增,反转录得到cDNA;新鲜稀释阳性模板,取不同梯度上机,得出RT-PCR标准曲线;上样,每个样本2个指标,每个指标3个重复孔。 "
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