Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats

doi:10.3969/j.issn.2095-4344.2768

Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (26): 4213-4217.doi: 10.3969/j.issn.2095-4344.2768

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Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats

Wu Zhen¹, Ma Wei², Zang Chenghao¹, Liu Kuangpin², Liu Wei², Liu Jie², Liang Yu², Li Chunyan², Chen Zhiming¹, Ru Jin¹, Fan Chuming¹, Yang Jinwei¹, Guo Jianhui¹, Li Liyan²

¹The Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China; ²Kunming Medical University, Kunming 650500, Yunnan Province, China

Received:2019-12-27 Revised:2020-01-04 Accepted:2020-02-19 Online:2020-09-18 Published:2020-09-02
Contact: Li Liyan, MD, Professor, Kunming Medical University, Kunming 650500, Yunnan Province, China Guo Jianhui, Professor, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China Yang Jinwei, Master, Attending physician, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
About author:Wu Zhen, Master candidate, the Affiliated Hospital of Kunming University of Science and Technology, the First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan Province, China
Supported by:
the National Natural Science Foundation of China, No. 31560295; Yunnan Provincial Science and Technology Department & Kunming Medical University Joint Special Key Project, No. 2018FE001 (-163) and 2019FE001 (-179); Yunnan Cardiovascular Disease Imaging Research Center, No. 2017NS254 and 2018NS0270 ; Yunnan Provincial Innovation Team, No. 2019HC022; Yunnan Provincial Ten Thousand Talent Project, No. (YNWR-MY-2018-015); the Major Scientific and Technological Achievement Cultivation Project of Kunming Medical University, No. CGPY201802

Abstract

Abstract:

BACKGROUND: Previous studies have found that panax notoginseng saponins have a certain protective effect on immunological liver injury in mice.

OBJECTIVE: To explore the therapeutic effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats.

METHODS: Experimental liver fibrosis model was made by carbon tetrachloride in male Sprague-Dawley rats. Then 30 g/L notoginsenoside R1 (60 mg/kg) was given once daily for 4 and 6 weeks in the treatment group. Rats in the control and model group were given distilled water of the same volume. Histopathological observation with hematoxylin-eosin staining and Masson’s trichrome staining was used to evaluate the changes of liver structure and fibrosis degree. The expression of collage type I, α-smooth muscle actin and transforming growth factor-β1 mRNA of hepatic tissue was measured by qRT-PCR method. The experimental protocol was approved by the Animal Experiment Ethics Committee of Kunming Medical University (approval No. KMMU2018018).

RESULTS AND CONCLUSION: Liver histopathology showed that notoginsenoside R1 improved the degree of liver fibrosis. The expression levels of collagen type I, α-smooth muscle actin and transforming growth factor-β1 mRNA were reduced significantly in the treatment group compared with the model group (P < 0.05). But there was no significant difference after 4 and 6 weeks of treatment with notoginsenoside R1. Overall findings indicate that notoginsenoside R1 can slow down the progression of carbon tetrachloride-induced liver fibrosis in rats to a certain extent.

Key words: notoginsenoside R1, carbon tetrachloride, liver fibrosis, collagen type I (Col-1), α-smooth muscle actin, transforming growth factor β1

CLC Number:

Wu Zhen, Ma Wei, Zang Chenghao, Liu Kuangpin, Liu Wei, Liu Jie, Liang Yu, Li Chunyan, Chen Zhiming, Ru Jin, Fan Chuming, Yang Jinwei, Guo Jianhui, Li Liyan.

Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(26): 4213-4217.

Figures/Tables 5

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Protective effect of notoginsenoside R1 on carbon tetrachloride-induced liver fibrosis in rats

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