Intraperitoneal transplantation of human umbilical cord mesenchymal stem cells in the treatment of acute liver injury in rats

doi:10.3969/j.issn.2095-4344.2013.49.010

Abstract

Abstract:

BACKGROUND: In vitro experiments have confirmed that human umbilical cord mesenchymal stem cells can be induced to differentiate into hepatocyte-like cells, thus which can be considered to function as liver repair.
OBJECTIVE: To observe the therapeutic effect of human umbilical cord mesenchymal stem cell transplantation on acute liver injuries in rats through in vivo animal experiments.
METHODS: Healthy Sprague-Dawley rats were randomly divided into three groups: normal control group without modeling, cell transplantation group, and PBS group. Rat models of acute liver injury were prepared by 10% CCl4-olive oil solution in the cell transplantation and PBS groups which were followed by intraperitoneal injection of 0.5 mL human umbilical cord mesenchymal stem cell suspension and 0.5 mL PBS, respectively.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that pathological changes related to acute liver injury appeared at 24 hours after intraperitoneal injection of CCl4. Then, the liver structure recovered at 7 days after cell transplantation, but it did not recover till the 14th day after PBS injection. Compared with the normal control group, serum alanine aminotransferase and aspartate aminotransferase levels were significantly increased in the other two groups (P < 0.05-0.01). In the cell transplantation group, the serum alanine aminotransferase and aspartate aminotransferase levels were significantly lower than those in the PBS group at 3 days after treatment (P < 0.05-0.01), and recovered normally after 7 days. Cells positive for anti-human nucleoprotein antibody were found in the portal area of liver tissues in the cell transplantation group after 3 days of transplantation, and then cells positive for anti-human albumin antibody appeared after 7 days. These findings indicate that intraperitoneal transplantation of human umbilical cord mesenchymal stem cells can improve liver function and repair injured liver tissues after acute liver injury in rats to some extent.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, stem cell transplantation, umbilical cord, models, animal, drug-induced liver injury, carbon tetrachloride

CLC Number:

R394.2

Zheng Xue-feng, Liu Xia. Intraperitoneal transplantation of human umbilical cord mesenchymal stem cells in the treatment of acute liver injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8527-8531.

Figures/Tables 5

References

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