Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (17): 2686-2693.doi: 10.3969/j.issn.2095-4344.2597
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Low-dose zoledronic acid regulates osteoclasts and
osteoblasts in the extraction socket of ovariectomized rats
Cheng Yuting1, Wu Chao1, Huang Xiaolin2, Li Fang1, Shi Qianhui1, Zhou Qian1, Hong Wei3, Wang Yong1, Liao Jian1
Received:
2019-08-21
Revised:
2019-08-22
Accepted:
2019-09-17
Online:
2020-06-18
Published:
2020-03-28
Contact:
Liao Jian, MD, Associate chief physician, Stomatological Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
About author:
Cheng Yuting, Master candidate, Stomatological Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China
Supported by:
CLC Number:
Cheng Yuting, Wu Chao, Huang Xiaolin, Li Fang, Shi Qianhui, Zhou Qian, Hong Wei, Wang Yong, Liao Jian.
Low-dose zoledronic acid regulates osteoclasts and
osteoblasts in the extraction socket of ovariectomized rats
2.5 免疫组织化学染色结果 RANKL蛋白属于分泌型蛋白,主要表达于细胞浆和细胞膜上。实验中有12只正常对照组大鼠低表达RANKL蛋白(±),8只模型组大鼠高表达RANKL蛋白(++和+++),9只治疗组大鼠RANKL蛋白表达下降(+),对照组、模型组、治疗组吸光度值分别为25±5, 26 604±3 415,2 823±641,组间比较差异有显著性意义(F=212,P < 0.001),见图4A,B,与对照组相比较,模型组的骨细胞胞浆和胞膜的RANKL表达较明显,而在唑来膦酸治疗后RANKL 表达明显减少;骨保护素蛋白的阳性部位与RANKL蛋白相同,10只正常对照组大鼠高表达骨保护素蛋白(++和+++),8只模型组大鼠低表达骨保护素蛋白(±),9只大鼠用药后骨保护素蛋白表达上升(++),对照组、模型组、治疗组吸光度值分别为17 691±5 287,407±365,11 610± 6 879,组间比较差异有显著性意义(F=12.23,P < 0.002)(图4A,B),对照组的骨保护素表达与其他组相比较略为明显,模型组阳性颗粒最少,骨保护素表达量最少;NF-KB p65蛋白主要位于细胞浆和细胞核上,有11只正常对照组大鼠低表达NF-κB蛋白(±),9只模型组大鼠高表达NF-κB蛋白(++和+++),9只大鼠用药后NF-κB蛋白表达下降(+),对照组、模型组、治疗组吸光度值分别为3 175±437,22 532±3 500,4 181±2 055,组间比较差异有显著性意义(F=85.51,P < 0.001),见图4A,B,模型组表达较其他组明显,用药后的治疗组与对照组无明显差异,表达较少。 "
2.6 Western Blot结果 与对照组相比较,模型组的RANKL、NF-κB蛋白高表达,用药后其表达量显著降低,甚至比正常大鼠的表达量略低些,对照组、模型组、治疗组的RANKL、NF-κB与内参β-Actin的灰度比值分别为0.305 8±0.081 1,0.732 0±0.054 9,0.345 7±0.034 7,组间比较差异有显著性意义(F=46.19,P < 0.001)和 0.374 7±0.082 4,0.892 6±0.108 0,0.281 7±0.072 9,组间比较差异有显著性意义(F=41.02,P < 0.001),见图5,模型组表达较其他组明显,用药后的治疗组与对照组比较差异无显著性意义。 "
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