Reduction for developmental dysplasia of the hip in rabbits: expression levels of Caspase-3 and Bcl-2 in acetabular chondrocytes

doi:10.3969/j.issn.2095-4344.0822

Abstract

Abstract:

BACKGROUND: Abnormal increase in the number of apoptotic articular chondrocytes can induce cartilage degeneration, further deteriorate and develop into osteoarthritis.
OBJECTIVE: To observe the expression of apoptosis-related factors in the treatment of developmental dysplasia of the hip by reduction.
METHODS: Sixty 28-day-old New Zealand white rabbits (either sex) were randomly selected, then the right hind limbs were fixed with tubular gypsum in flexion of hip and extension of knee for 8 weeks to establish an animal model of developmental dysplasia of the hip, and the left hind limbs served as controls. Subsequently, the successful models were judged by the X-ray film, acetabular index, and dislocation of the femoral. Forty-eight model rabbits were equally randomized into group A (8 weeks of fixation), group B (10 weeks of fixation), group C (embolia at 2 weeks after 8 weeks of fixation). The morphology of chondrocytes at the acetabular cartilage was observed after hematoxylin-eosin staining. The expression levels of Caspase-3 and Bcl-2 in chondrocytes were detected by immunohistochemistry. The apoptosis rate in the acetabular cartilage was detected by TUNEL method, and the correlation was analyzed.
RESULTS AND CONCLUSION: (1) The acetabular index in the groups A, B and C was significantly higher than that in the control group (P < 0.05). (2) The morphology of chondrocytes in control group after hematoxylin-eosin staining was regular, and the groups A and B appeared with vacuolate cells, part of the structure disappeared, and the structures of acetabular and femoral head articular cartilage were complete, with less vacuole in the group C. (3) TUNEL method found that the apoptosis rate in the group C was lower than that in the group B, but higher than that in the control group (P < 0.05), indicating the decreased apoptotic rate after reduction. (4) Correlation analysis showed that the apoptosis rate was positively correlated with the expression level of Caspase-3 and negatively correlated with Bcl-2 expression. (5) To conclude, the reduction group shows a highly expressed Bcl-2, decreased expression of Caspase-3 and reduced apoptosis rate. Therefore, the reduction method can reduce the apoptosis rate of acetabular chondrocytes in developmental dysplasia of the hip, delay the degeneration of cartilage, and slow the course of disease progression.

Key words: tissue construction, hip dislocation, reduction, cartilage, cell apoptosis, Bcl-2, Caspase-3, developmental dysplasia of the hip, hip dysplasia

CLC Number:

Xiong Fei1, Wei Yishan2. Reduction for developmental dysplasia of the hip in rabbits: expression levels of Caspase-3 and Bcl-2 in acetabular chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 4974-4978.

Figures/Tables 5

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