Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (10): 1520-1525.doi: 10.3969/j.issn.2095-4344.1574
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You Qi1, Duan Xiaojun2, Zhang Jun1, Jin Ying1, Peng Xu1, Ge Zhen1, Liu Yi1
Received:
2018-10-15
Online:
2019-04-08
Published:
2019-04-08
Contact:
Liu Yi, Professor, Department of Joint Surgery, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China.
About author:
You Qi, Master candidate, Department of Joint Surgery, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
Supported by:
the National Natural Science Foundation of China, No. 81071484 (to DXJ); the Combined Foundation of Science & Technology Department of Guizhou Province, No. LH[2017]7015
CLC Number:
You Qi, Duan Xiaojun, Zhang Jun, Jin Ying, Peng Xu, Ge Zhen, Liu Yi. Feasibility of repairing articular cartilage defects with particulated juvenile cartilage allograft[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(10): 1520-1525.
2.3.3 组织学观察 苏木精-伊红染色结果显示,空白组无明显填充物,仅有少量纤维组织样物填充;实验组缺损被软骨样组织填充,与周围正常软骨整合良好。甲苯胺蓝染色结果显示,空白组缺损未见明显着色的修复组织;实验组缺损可见蓝染的修复组织填充,着色强度与周围正常软骨组织类似,界面整合良好。番红-固绿染色结果显示,空白组缺损中修复组织着色不明显;实验组缺损中可见红染的修复组织填充,且着色强度与周围正常软骨类似,潮线结构完整,软骨与骨界面整合良好。Ⅱ型胶原免疫组织化学染色结果显示,空白组缺损中未见明显着色的Ⅱ型胶原;实验组缺损中可见着色阳性的Ⅱ型胶原,且着色强度与周围正常软骨组织类似。实验组与对照组关节软骨缺损组织学观察结果,见图5。"
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