Resveratrol regulates the expression of sterol regulatory element-binding protein-2 and matrix metalloproteinase 13 in mouse chondrocytes

doi:10.3969/j.issn.2095-4344.0577

Abstract

Abstract:

BACKGROUND: Recent studies suggest that sterol regulatory element-binding protein (SREBP)-2 and matrix metalloproteinase 13 (MMP-13) are up-regulated in chondrocytes of patients with osteoarthritis. The role of resveratrol in the prevention and treatment of osteoarthritis is considered to be involved in the reduction of chondrocyte apoptosis and synovial inflammation.
OBJECTIVE: To further investigate the effect of resveratrol on the expression of MMP-13 and SREBP-2 in chondrocytes.
METHODS: Mouse knee chondrocytes were isolated and cultured in vitro, and identified by immunofluorescence staining with collagen type II. The experiment was divided into three groups according to the different addition materials: blank control group, lipopolysaccharide group (1 mg/L) and resveratrol group (100 μmol/L resveratrol plus 1 mg/L lipopolysaccharide). The expression level of MMP-13 protein in chondrocytes was detected by western blot assay. The expression level of SREBP-2 in chondrocytes was determined by ELISA method.
RESULTS AND CONCLUSION: Fluorescence microscopy showed that the number of cells positive for collagen type II accounted for more than 95%, confirming that the cultured cells were chondrocytes. Compared with the blank control group, the expression levels of SREBP-2 and MMP-13 in the lipopolysaccharide group were significantly increased, and the expression levels of SREBP-2 and MMP-13 in the resveratrol group were lower than those in the lipopolysaccharide group, but they were still higher than those in the blank control group. In summary, resveratrol can inhibit the expression of SREBP-2 and MMP-13 in mouse chondrocytes.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Chondrocytes, Sterol Regulatory Element Binding Protein 2, Matrix Metalloproteinases

CLC Number:

R318

Yuan Hao, Zeng Hui, Chen Ke, Yu Fei, Lin Jianjing. Resveratrol regulates the expression of sterol regulatory element-binding protein-2 and matrix metalloproteinase 13 in mouse chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(36): 5772-5777.

Figures/Tables 3

References

[1] Liu Y,Zhang H,Liang N,et al.Prevalence and associated factors of knee osteoarthritis in a rural Chinese adult population: an epidemiological survey. BMC Public Health. 2016;16(1):94.

[2] Ackerman IN, Osborne RH.Obesity and increased burden of hip and knee joint disease in Australia: results from a national survey.BMC Musculoskelet Disord.2012;13:254.

[3] Raederstorff D,Kunz I,Schwager J.Resveratrol, from experimental data to nutritional evidence: the emergence of a new food ingredient. Ann N Y Acad Sci.2013;1290:136-141.

[4] Yu W, Fu YC, Wang W. Cellular and molecular effects of resveratrol in health and disease. J Cell Biochem.2012; 113(3):752-759.

[5] Nakata R, Takahashi S, Inoue H. Recent advances in the study on resveratrol. Biol Pharm Bull.2012;35(3):273-279.

[6] Maepa M,Razwinani M,Motaung S.Effects of resveratrol on collagen type II protein in the superficial and middle zone chondrocytes of porcine articular cartilage. J Ethnopharmacol. 2016;178:25-33.

[7] 夏睿,董启榕. MMP13在骨性关节炎患者滑膜细胞中的表达[J]. 苏州大学学报(医学版),2006,26(5):815-817.

[8] Kostopoulou F, Gkretsi V, Malizos K N, et al. Central role of SREBP-2 in the pathogenesis of osteoarthritis. PLoS One. 2012;7(5):e35753.

[9] Qiu XM, Jin CT, Wang W.Association between single nucleotide polymorphisms of sterol regulatory element binding protein-2 gene and risk of knee osteoarthritis in a Chinese Han population. J Int Med Res.2014;42(2):320-328.

[10] Pullig O, Kladny B, Weseloh G, et al.[Metabolic activation of chondrocytes in human osteoarthritis. Expression of type II collagen].Z OrthopIhre Grenzgeb.1999;137(1):67-75.

[11] 刘弼,肖德明,雷鸣,等. 白藜芦醇对硝普钠诱导的软骨细胞凋亡bax及bcl-2表达的影响[J]. 中国矫形外科杂志, 2012,20(13): 1235-1238.

[12] Shakibaei M, John T, Seifarth C, et al. Resveratrol inhibits IL-1 beta-induced stimulation of caspase-3 and cleavage of PARP in human articular chondrocytes in vitro. Ann N Y Acad Sci. 2007;1095:554-563.

[13] Chang CH, Hsu YM, Chen YC, et al.Anti-inflammatory effects of hydrophilic and lipophilic statins with hyaluronic acid against LPS-induced inflammation in porcine articular chondrocytes. J Orthop Res.2014;32(4):557-565.

[14] 刘维,吴沅皞.青藤碱对炎性刺激下体外培养关节软骨细胞的保护作用[J]. 国际免疫学杂志,2011,34(5):309-312.

[15] Suri P, Morgenroth DC, Hunter DJ. Epidemiology of osteoarthritis and associated comorbidities. PM R.2012; 4(5 Suppl):S10-S19.

[16] Davies-Tuck ML, Hanna F, Davis SR, et al. Total cholesterol and triglycerides are associated with the development of new bone marrow lesions in asymptomatic middle-aged women - a prospective cohort study. Arthritis Res Ther.2009;11(6): R181.

[17] Miller GD, Jenks MZ, Vendela M,et al.Influence of weight loss, body composition, and lifestyle behaviors on plasma adipokines: a randomized weight loss trial in older men and women with symptomatic knee osteoarthritis. J Obes.2012; 2012:708505.

[18] Iwata M, Ochi H, Hara Y, et al. Initial responses of articular tissues in a murine high-fat diet-induced osteoarthritis model: pivotal role of the IPFP as a cytokine fountain. PLoS One. 2013;8(4):e60706.

[19] Infantino V, Iacobazzi V, De Santis F, et al. Transcription of the mitochondrial citrate carrier gene: role of SREBP-1, upregulation by insulin and downregulation by PUFA. BiochemBiophys Res Commun.2007;356(1):249-254.

[20] Eberle D, Hegarty B, Bossard P, et al. SREBP transcription factors: master regulators of lipid homeostasis. Biochimie. 2004;86(11):839-848.

[21] 翁鉴,曾晖,肖德明,等. 固醇调节元件结合蛋白2在软骨细胞退变过程中的表达[J]. 中国组织工程研究, 2015,19(29):4624-4628.

[22] 张亚峰,王骏飞,陈东阳,等. 核因子κB信号转导途径在大鼠实验性骨性关节炎软骨细胞中被激活[J]. 中国临床康复, 2006, 10(37):71-73, 图34-图37.

[23] Lei M, Liu SQ, Liu YL. Resveratrol protects bone marrow mesenchymal stem cell derived chondrocytes cultured on chitosan-gelatin scaffolds from the inhibitory effect of interleukin-1beta. ActaPharmacol Sin.2008;29(11): 1350-1356.

[24] Torzilli PA, Bhargava M, Park S, et al. Mechanical load inhibits IL-1 induced matrix degradation in articular cartilage. Osteoarthritis Cartilage.2010;18(1):97-105.