Animal models of stress urinary incontinence: selection of appropriate construction methods of research objective-related animal models

doi:10.3969/j.issn.2095-4344.0252

Abstract

Abstract:

BACKGROUND: Animal models of stress urinary incontinence (SUI) can simulate the anatomical structure and pathological state of pelvic floor in female patients with SUI. Animal models are important for studies on the pathogenesis and therapeutic effect of SUI.
OBJECTIVE: To summarize the SUI modeling methods and research ideas, reveal the status and progress of establishing an animal model of SUI, and compare the advantages and disadvantages of various modeling methods, thereby providing the basis for ideal animal model construction and providing favorable reference for clinical research.
METHODS: Databases of CNKI and PubMed were retrieved using computer with the search time of January 2000 to August 2017. The keywords were “stress urinary incontinence and animal model” in English and Chinese, respectively. The articles addressing animal models rather than mechanism were excluded. Pathogenesis, establishment and pathological characteristics of animal models of SUI were summarized.
RESULTS AND CONCLUSION: A total of 128 articles were retrieved, the repetitive and obsolete articles were excluded, and then 37 eligible articles were included. The animal models of SUI mainly relay on the mechanical damage of the pelvic floor support organization. All kinds of models can only focus on some pathological causes of a disease, or pathological changes at a certain stage. Moreover, the maintenance time, advantages and disadvantages are different. So researchers must choose appropriate animal models according to their own requirements.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Urinary Incontinence, Stress, Models, Animal, Tissue Engineering

CLC Number:

R318

Wang Tian-xing1, Chen Yue-lai2, Yin Ping3, Xu Shi-fen3, Zheng Hui-min3 . Animal models of stress urinary incontinence: selection of appropriate construction methods of research objective-related animal models[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4557-4561.

Figures/Tables 2

2.1 纳入资料基本概况纳入文献内容符合标准的37篇文献中，文献[6-13]探讨了压力性尿失禁模型不同动物的选择和常用的造模方法，文献[14-22]具体描述了模拟产伤法建立压力性尿失禁动物模型，文献[23-27]描述了模拟产伤联合双侧卵巢切除法建立压力性尿失禁动物模型，文献[28-31]描述了阴部神经损伤建立压力性尿失禁动物模型，文献[32-34]描述了模拟尿道周围组织破坏法构建压力性尿失禁动物模型，文献[35-36]描述了较少使用的特殊类型建模方法。文献检索流程图见图1。 2.2 纳入文章的研究结果特征 2.2.1 压力尿失禁模型动物的选择国内外有关压力性尿失禁动物模型的选择较多，根据模型各自特点及研究目的不同，主要类型多为鼠类，也有兔、犬、猪、猴等。SD大鼠和Wistar大鼠是压力性尿失禁建模中最常用的动物，其繁殖力强使用经济，饲养方便利于长期观察研究，可有效模拟女性盆底解剖结构和压力性尿失禁发病机制。建立稳定方便的动物模型是进一步深入研究压力性尿失禁病理机制和治疗效应的重要步骤。方案成熟公认的压力性尿失禁动物模型建造方法主要有模拟产伤法、卵巢切除去势法、阴部神经损伤法、尿道周围组织破坏法等，各类动物模型的建模原理主要为采用机械性损伤盆底支撑结构，其稳定性和成功率各不相同，针对不同研究目的各有优缺点[6-13]。 2.2.2 模拟产伤法建立压力性尿失禁动物模型主要机制：通过模拟多次分娩、第二产程延长及产伤对盆底的支持结构如肌肉、韧带及神经等产生牵拉位移、压迫缺血或神经源性损伤。研究表明，阴道分娩时阴部神经损伤引起的盆底结构改变与尿失禁发生密切相关，经阴道分娩的女性比行剖腹产女性更易患压力性尿失禁[14-15]。在实验动物阴道内置气囊长时间压迫阴道壁"

References

[1] Abrams P, Cardozo L, Fall M, et al. The standardisation of terminology in lower urinary tract function: report from the standardisation sub-committee of the International Continence Society. Urology. 2003;61(1):37-49.

[2] Kobashi KC, Albo ME, Dmochowski RR, et al. Surgical Treatment of Female Stress Urinary Incontinence (SUI): AUA/SUFU Guideline. 2017. http://www.auanet.org/guidelines/stress-urinary-incontinence-(sui)-new-(aua/sufu-guideline-2017). 2017-05-20.

[3] Sinclair A J, Ramsay I N. The psychosocial impact of urinary incontinence in women. Obstetrician Gynaecologist. 2011;13(3): 143-148.

[4] 郑慧敏,徐世芬,尹平,等.电针治疗轻中度女性压力性尿失禁的近远期疗效观察[J].世界中西医结合杂志, 2015,10(2):191-193.

[5] Liu Z, Liu Y, Xu H, et al. Effect of Electroacupuncture on Urinary Leakage Among Women With Stress Urinary Incontinence: A Randomized Clinical Trial. JAMA. 2017; 317(24):2493-2501.

[6] Hijaz A, Daneshgari F, Sievert KD, et al. Animal models of female stress urinary incontinence. J Urol. 2008;179(6): 2103-2110.

[7] Jiang HH, Damaser MS. Animal models of stress urinary incontinence. Handb Exp Pharmacol. 2011;(202):45-67.

[8] Herrera-Imbroda B, Lara MF, Izeta A, et al. Stress urinary incontinence animal models as a tool to study cell-based regenerative therapies targeting the urethral sphincter. Adv Drug Deliv Rev. 2015;82-83: 106-116.

[9] Burdzińska A, Crayton R, Dybowski B, et al. Urethral distension as a novel method to simulate sphincter insufficiency in the porcine animal model. Int J Urol. 2012; 19(7):676-682.

[10] Gill BC, Damaser MS, Vasavada SP, et al. Stress incontinence in the era of regenerative medicine: reviewing the importance of the pudendal nerve. J Urol. 2013;190(1): 22-28.

[11] Koike Y, Furuta A, Suzuki Y, et al. Pathophysiology of urinary incontinence in murine models. Int J Urol. 2013;20(1):64-71.

[12] Sajadi KP, Gill BC, Damaser MS. Neurogenic aspects of stress urinary incontinence. Curr Opin Obstet Gynecol. 2010;22(5):425-429.

[13] Baek M, Paick SH, Jeong SJ, et al. Urodynamic and histological changes in a sterile rabbit vesicoureteral reflux model. J Korean Med Sci. 2010;25(9):1352-1358.

[14] Gill BC, Moore C, Damaser MS. Postpartum stress urinary incontinence: lessons from animal models. Expert Rev Obstet Gynecol. 2010;5(5):567-580.

[15] Goldberg RP, Kwon C, Gandhi S, et al. Urinary incontinence among mothers of multiples: the protective effect of cesarean delivery. Am J Obstet Gynecol. 2003;188(6):1447-1450; discussion 1450-1453.

[16] Pan HQ, Kerns JM, Lin DL, et al. Increased duration of simulated childbirth injuries results in increased time to recovery. Am J Physiol Regul Integr Comp Physiol. 2007; 292(4):R1738-1744.

[17] Phull HS, Pan HQ, Butler RS, et al. Vulnerability of continence structures to injury by simulated childbirth. Am J Physiol Renal Physiol. 2011;301(3):F641-649.

[18] Woo LL, Hijaz A, Pan HQ, et al. Simulated childbirth injuries in an inbred rat strain. Neurourol Urodyn. 2009;28(4):356-361.

[19] Boncher N, Vricella G, Kavran M, et al. Setting a new standard: updating the vaginal distention translational model for stress urinary incontinence. Neurourol Urodyn. 2012;31(1):190-194.

[20] Sievert KD, Bakircioglu ME, Tsai T, et al. The effect of labor and/or ovariectomy on rodent continence mechanism--the neuronal changes. World J Urol. 2004;22(4):244-250.

[21] Vricella GJ, Tao M, Altuntas CZ, et al. Expression of monocyte chemotactic protein 3 following simulated birth trauma in a murine model of obesity. Urology. 2010;76(6):1517.e12-17.

[22] 汤剑明,洪莉,刘成,等.压力性尿失禁小鼠模型的建立及效果评价[J].中华妇幼临床医学杂志(电子版),2016,12(5):540-546.

[23] Kane DD, Kerns JM, Lin DL, et al. Early structural effects of oestrogen on pudendal nerve regeneration in the rat. BJU Int. 2004;93(6): 870-878.

[24] Qiang F, Guo-long L. Comparative study of three rat models of stress urinary incontinence. Bosn J Basic Med Sci. 2011; 11(2):87-90.

[25] Sievert KD, Emre Bakircioglu M, Tsai T, et al. The effect of simulated birth trauma and/or ovariectomy on rodent continence mechanism. Part I: functional and structural change. J Urol. 2001;166(1):311-317.

[26] Resplande J, Gholami SS, Graziottin TM, et al. Long-term effect of ovariectomy and simulated birth trauma on the lower urinary tract of female rats. J Urol. 2002;168(1):323-330.

[27] Kuo HC. Effects of vaginal trauma and oophorectomy on the continence mechanism in rats. Urol Int. 2002;69(1):36-41.

[28] Damaser MS, Broxton KC, Ferguson C, et al. Functional and neuroanastomical effects of vaginal distension and pudendal nerve crush in the female rat. J Urol. 2003;170(3):1027-1031.

[29] 王皓羽,杜小文,徐剑炜,等.压力性尿失禁动物模型的建立及效果评价[J].中国组织工程研究与临床康复, 2010,14(11): 1959-1962.

[30] Ahn H, Lin DL, Esparza N, et al. Short-term timecourse of bilateral pudendal nerve injury on leak-point pressure in female rats. J Rehabil Res Dev. 2005;42(1):109-114.

[31] Peng CW, Chen JJ, Chang HY, et al. External urethral sphincter activity in a rat model of pudendal nerve injury. Neurourol Urodyn. 2006;25(4):388-396.

[32] Chermansky CJ, Cannon TW, Torimoto K, et al. A model of intrinsic sphincteric deficiency in the rat: electrocauterization. Neurourol Urodyn. 2004;23(2):166-171.

[33] Hong SH, Piao S, Kim IG, et al. Comparison of three types of stress urinary incontinence rat models: electrocauterization, pudendal denervation, and vaginal distension. Urology. 2013; 81(2):465.e1-6.

[34] Kefer JC, Liu G, Daneshgari F. Pubo-urethral ligament injury causes long-term stress urinary incontinence in female rats: an animal model of the integral theory. J Urol. 2009;181(1): 397-400.

[35] Takahashi S, Chen Q, Ogushi T, et al. Periurethral injection of sustained release basic fibroblast growth factor improves sphincteric contractility of the rat urethra denervated by botulinum-a toxin. J Urol. 2006;176(2):819-823.

[36] Kwon D, Kim Y, Pruchnic R, et al. Periurethral cellular injection: comparison of muscle-derived progenitor cells and fibroblasts with regard to efficacy and tissue contractility in an animal model of stress urinary incontinence. Urology. 2006; 68(2):449-454.

[37] Yoshimura N, Miyazato M. Neurophysiology and therapeutic receptor targets for stress urinary incontinence. Int J Urol. 2012;19(6):524-537.