Berberine promotes the in vitro migration of bone marrow mesenchymal stem cells via activation of PI3K/AKT pathway

doi:10.3969/j.issn.2095-4344.0967

Abstract

Abstract:

BACKGROUND: Berberine as the main alkaloid of herbal Coptis chinensis has many pharmacological effects on bone marrow mesenchymal stem cells (BMSCs).
OBJECTIVE: To explore the effects and mechanism of berberine on the in vitro migration of BMSCs.
METHODS: Rat BMSCs were isolated and cultured by a whole bone marrow assay, and prepared into 1.5×10⁹/L. Berberine was dissolved by dimethyl sulfoxide, and diluted by α-MEM containing 1 % fetal bovine serum into the concentrations of 1, 3, 10 μmol/L. Cell migration was assessed using a Transwell chamber and cell scratch assay. Western blot assay was used to test the regulatory effect of berberine on p-AKT pathway.
RESULTS AND CONCLUSION: Berberine had no effect on the cell viability of BMSCs. Berberine at 10 μmol/L significantly promoted the migration of BMSCs. The cell scratch assay shared consistent findings with the Transwell assay. Addition of berberine up-regulated the p-AKT expression in BMSCs, while LY294002, the inhibition of p-AKT, could effectively reverse the pro-migration effects of berberine. Overall, berberine can promote the migration of BMSCs via the activation of PI3K/AKT pathway.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Berberine, Bone Marrow, Mesenchymal Stem Cells, Cell Migration Assays, Tissue Engineering

CLC Number:

R394.2

Li Qiong-jing, Luo Qi. Berberine promotes the in vitro migration of bone marrow mesenchymal stem cells via activation of PI3K/AKT pathway[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(29): 4620-4624.

Figures/Tables 3

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