Puerarin promotes osteogenesis of osteoblasts compounded with beta-tertiary calcium phosphate

doi:10.3969/j.issn.2095-4344.0388

Abstract

Abstract:

BACKGROUND: Puerarin is the major bioactive ingredient isolated from the root of the Pueraria lobata (Willd.) Ohwi, which is a kind of isoflavones. Isoflavones has been shown to promote the proliferation and differentiation of osteoblasts.
OBJECTIVE: To observe the effect of puerarin at different concentrations on the osteogenic activity of β-tertiary calcium phosphate (β-TCP) complex, and to investigate the effect of puerarin on osteogenesis in vivo.
METHODS: Osteoblasts isolated from calvarial of newborn rabbits were cultured in vitro with different concentrations of puerarin. The alkaline phosphotase activity and mineral node formation were determined by alkaline phosphatase kit and alizarin red staining. The osteoblasts cultured with the optimal concentration of puerarin (group A) or without puerarin (group B) were seeded on β-TCP, respectively. Osteoblast activity was determined using MTT assay. Scaffold complexes in each group were implanted into the rabbit dorsal muscles. Osteogenesis was analyzed by histological analysis.
RESULTS AND CONCLUSION: The alkaline phosphatase activity and mineral nodules were significantly increased in osteoblasts cultured with puerarin, and 1×10-6 mol/L puerarin could significantly incresase increase the osteoblast activity in the scaffold. After the β-TCP composite scaffolds cultured with 1×10-6 mol/L puerarin were implanted into the rabbit dorsal muscles, the newly formed bone was significantly increased. In summary, puerarin enhances the osteogenic effect of β-TCP osteoblast complex in vivo, which can be used to promote osteogenesis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoblasts, Calcium Phosphates, Tissue Engineering

CLC Number:

R318

Chang Zai-ping1, Yang Hai-long2, Zhang Ming-yu3. Puerarin promotes osteogenesis of osteoblasts compounded with beta-tertiary calcium phosphate[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4447-4451.

Figures/Tables 1

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