Preparation and biocompatibility of an electroactive polyaniline/poly(lactic-acid) scaffold

doi:10.3969/j.issn.2095-4344.0755

Abstract

Abstract:

BACKGROUND: Previous studies have confirmed that polyaniline/poly(lactic-acid) scaffold could promote the growth of neural stem cell processes in the condition of electrical stimulation.

OBJECTIVE: To prepare an electroactive polyaniline/poly(lactic-acid) scaffold and to investigate its biocompatibility with rat olfactory ensheathing cells.

METHODS: Olfactory ensheathing cells of Sprague-Dawley neonatal rats were inoculated onto the polyaniline/poly(lactic acid) film (experimental group) and poly lysine coating slides (control group). Cell proliferation and cell adhesion rate were detected, and the cells were observed with immunofluorescence histochemistry.

RESULTS AND CONCLUSION: (1) Smooth and even polyaniline/poly(lactic acid) film was visible under electron microscopy, with a grid structure formed by fine fibers, and its conductivity was 1.5×10^-5 S/cm. (2) At 1, 3, 5, 7 days after co-culture, the cell relative growth rates were all above 90%, and the cytotoxicity was graded I. (3) At 7 days after co-culture, the cell adhesion rate in the experimental group was significantly higher than that in the control group (P < 0.05). (4) At 7 days after co-culture, immunofluorescence results showed that there was no obvious difference between the two groups in the number or area of S-100 positive cells (P > 0.05), while the cell perimeter in the experimental group was significantly higher than that in the control group (P < 0.05). To conclude, the prepared polyaniline/poly(lactic acid) film has good biocompatibility with olfactory ensheathing cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Spinal Cord Injuries, Tissue Engineering

CLC Number:

R318

Wang Ling-ling, Cui Zhi-ming, Xu Guan-hua, Li Wei-dong, Bao Guo-feng, Sun Yu-yu, Chen Jia-jia, Zhang Jin-long. Preparation and biocompatibility of an electroactive polyaniline/poly(lactic-acid) scaffold[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(22): 3557-3562.

Figures/Tables 7

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