Vascular endothelial growth factor-modified bladder acellular collagen matrix for repair of urethral defects

doi:10.3969/j.issn.2095-4344.0921

Abstract

Abstract:

BACKGROUND: Modification by vascular endothelial growth factors (VEGF) contributes to the repair of urethral

defects using bladder acellular collagen matrix scaffolds. However, there is a dispute in different experimental results.

OBJECTIVE: To investigate the effect of VEGF-modified bladder acellular collagen matrix in the repair of rabbit urethral defects.

METHODS: Forty-eight rabbits were randomized into four groups (n=12 per group): no intervention was done in normal group; an animal model of urethral defect was made in the model, control and experimental groups, followed by implantation of nothing, bladder acellular collagen matrix and VEGF-modified bladder acellular collagen matrix, respectively. Rabbit urinary flow rate and maximal urethral pressure were measured. Hematoxylin-eosin staining was performed on the urethral defects to observe the urethral tissue repair and to detect the number of microvessels at 1 month after modeling.

RESULTS AND CONCLUSION: (1) At 1 month after modeling, the urinary flow rate and maximal urethral pressure in the experimental group had no statistical significance compared with the normal group (P > 0.05), but were significantly higher than those in the model and control groups (P < 0.05). The urinary flow rate and maximal urethral pressure in the model group were significantly lower than those in the normal and control groups (P < 0.05). (2) At 1 month after modeling, the urethral tissue in the normal group was clear with the epithelial cells being arranged regularly. The urethral tissues in the model group were relatively ambiguous shown by hematoxylin-eosin staining, the epithelial cells were arranged disorderly, and a large amount of inflammatory cells in the repair tissue were infiltrative. The epithelium in the experimental group was well repaired, incompletely degraded collagen matrix and new blood vessels were detected, and no inflammatory cell infiltration was observed. The mucosal epithelium in the control group was generally repaired, but no obvious neovascularization was visible, with a few inflammatory cell infiltrations. (1) At 1 month after modeling, the number of microvessels in the experimental group was significantly higher than that in the other three groups (P < 0.05), and the number of microvessels in the control group was also higher than that in the model group (P < 0.05). Overall, the use of VEGF-modified bladder acellular collagen matrix in the repair of rabbit urethral defects can promote local neovascularization and improve the environment for promoting urethral regeneration.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Vascular Endothelial Growth Factors, Urinary Bladder, Urethra, Tissue Engineering

CLC Number:

R318

Li Gui-li, Zhao Qi-lin. Vascular endothelial growth factor-modified bladder acellular collagen matrix for repair of urethral defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.0921.

Figures/Tables 3

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