17Beta-estradiol combined with icariin promotes the osteogenic differentiation of bone marrow mesenchymal stem cells from Sprague-Dawley rats

doi:10.3969/j.issn.2095-4344.0879

Abstract

Abstract:

BACKGROUND: Previous studies have shown that 17β-estradiol and icariin can both promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), but their combined use is rarely reported.
OBJECTIVE: To observe the effects of 17β-estradiol combined with icariin on the proliferation and osteogenic differentiation of BMSCs.
METHODS: BMSCs were isolated and cultured by whole-bone marrow adherence separation. Surface markers (CD29, CD45, CD90) of BMSCs were identified by flow cytometry. Cultured cells were divided into control group, 17β-estradiol group, icariin group, combined induction group, followed by corresponding treatments. At 7, 14 and 21 days after osteogenic induction, alkaline phosphatase activity, levels of collagen type I and osteocalcin were detected, and alizarin red staining was performed to observe the formation of calcium nodules.
RESULTS AND CONCLUSION: The osteogenic ability of BMSCs treated with 17β-estradiol combined with icariin was enhanced. After 7 days of osteogenic induction, the alkaline phosphatase activity was detected in all groups, and ranked as follows: combined induction group > 17β-estradiol group > icariin group > control group. After 14 days of osteogenic induction, the expression of collagen type I was observed in all groups and ranked as follows: combined induction group > 17β-estradiol group=icariin group > control group. After 21 days of osteogenic induction, osteocalcin was expressed in all groups and ranked as follows: induction group > 17β-estradiol group > icariin group > control group. The number of calcium nodules as determined by alizarin red staining was ranked as follows: induction group > 17β-estradiol group=icariin group > control group. Therefore, 17β-estradiol and Icariin have synergistic effects to induce BMSCs to differentiate into bone cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Epimedium, Estradiol, Cell Differentiation, Tissue Engineering

CLC Number:

R394.2

Yang Yan-bing, Jin Xian-hui, Wang Hai-ping . 17Beta-estradiol combined with icariin promotes the osteogenic differentiation of bone marrow mesenchymal stem cells from Sprague-Dawley rats[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(21): 3299-3303.

Figures/Tables 2

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