Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6767-6773.doi: 10.3969/j.issn.2095-4344.2012.36.021

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Amniotic mesenchymal stem cells differentiate into motor neuron precursor cell

Hu Wei1, 2, Yang Feng1, Tang You-jia1, Yang Bo2, Guan Fang-xia3   

  1. 1Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang 332000, Jiangxi Province, China;
    2Department of Neurosurgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China;
    3Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, Henan Province, China
  • Received:2011-10-02 Revised:2011-11-16 Online:2012-09-02 Published:2012-09-02
  • Contact: 杨波，博士，主任医师，教授，博士生导师，郑州大学第一附属医院神经外科，河南省郑州市450052 yangbo96@126.com
  • About author:Hu Wei☆, Doctor, Associate chief physician, Department of Neurosurgery, First People's Hospital of Jiujiang City, Jiujiang 332000, Jiangxi Province, China huwei18120@sina.com

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Abstract:

BACKGROUND: The reports about neural differentiation of adult stem cells induced by extracellular matrix and cytokines are rarely reported.
OBJECTIVE: To explore the neural differentiation of human amniotic mesenchymal stem cells in vitro induced by natural membrane-like extracelluler martrix.
METHODS: Amniotic mesenchymal stem cells were isolated from healthy human amnion, and membrane-like extracellular matrix was made by enzyme digestion and chemical method and biocompatibility was detected. The cells were divided into two groups. In the experimental group, the cells were seeded in 24-well plates collated with capsule-like matrix slides and culture medium was changed. In the control group, membrane-like matrix was removed and the other procedures were the same as the experimental group.
RESULTS AND CONCLUSION: After step-by-step induction in the experimental group, the expressions of neuron-specific enolase and rabbit anti-human synaptic proteins were increased and the expression of neuroglial fibrillary acidic protein was decreased, the expression of rabbit anti-human synaptic protein in the experimental group was significantly higher than that in the control group. In the control group, the expression of neuron-specific enolase was increased significantly and the expression of rabbit anti-human synaptic protein and neuroglial fibrillary acidic protein were not changed. Passage 1 amniotic mesenchymal stem cells could express the marker of embryonic stem cells and neural progenitor cells, the transcription factors in the experimental group were changed after induction. It indicates that the extracellular matrix extracted by step-by-step method had a good biocompatibility, which could promote the mature of synapses during the differentiation of amniotic mesenchymal stem cells. Induction by step-by-step method could enrich neural progenitor cells in amniotic mesenchymal stem cells and promote them to differentiate into motor neuron-like precursor cells.

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