Preparation of icariin-poly(lactic acid)/hydroxyapatite composite bone-graft substitutes by supercritical carbon dioxide method

doi:10.3969/j.issn.2095-4344.0786

Abstract

Abstract:

BACKGROUND: Our previous findings have shown that poly(lactic acid)/hydroxyapatite (PLA/HA) composite biomaterials prepared by the supercritical carbon dioxide method have good physiochemical properties and biocompatibility. However, the composite materials only have osteoconductivity but no osteoinductivity.

OBJECTIVE: To prepare icariin-PLA/HA (IC-PLA/HA) composite biomaterials with good osteoconduction and osteoinduction.

METHODS: The supercritical carbon dioxide method was used to prepare IC-PLA/HA composite biomaterials containing 10^-4, 10^-5, 10^-6 mmol/g IC, named as IC-PLA/HA(1 000), IC-PLA/HA(100) and IC-PLA/HA(10). PLA/HA composite material served as controls. Biomechanical properties, porosity, sustained release characteristics were detected, and scanning electron microscope observation was performed, in order to screen out the optimal IC content in the composite materials.

RESULTS AND CONCLUSION: (1) Compressive strength and elastic modulus of IC-PLA/HA(1 000), IC-PLA/HA(100) and IC-PLA/HA(10) showed no difference from those of PLA/HA. (2) The porosity of all the composite materials was over 75%, and there was still no difference among groups. (3) The IC release from IC-PLA/HA was faster within the first 3 days, and then reduced gradually. However, after 7 days, the IC release plateaued, and the IC release amount from the IC-PLA/HA(100) was close to 10^-7 mol/L that had been confirmed to be an effective and safe concentration in the previous experiments. (4) Under the scanning electron microscope, HA and PLA were mixed homogeneously and IC was difficult to be identified. The pore size of the IC-PLA/HA(100) ranged from 50 μm to 150 μm. Overall, the IC-PLA/HA composite biomaterials have good mechanical and sustained-release properties.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Drugs, Chinese Herbal, Hydroxyapatites, Biomimetic Materials, Tissue Engineering

CLC Number:

R318

Zhang Yu-min, Wang Jian-ru, Han Bo, Niu Xiao-jun. Preparation of icariin-poly(lactic acid)/hydroxyapatite composite bone-graft substitutes by supercritical carbon dioxide method[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(18): 2789-2794.

Figures/Tables 4

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