Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (36): 6741-6747.doi: 10.3969/j.issn.2095-4344.2012.36.016

Previous Articles     Next Articles

Effect of granulocyte colony-stimulating factor on the expression of hippocampal apoptosis-related protein in rats with vascular dementia

Li Xiao-yun1, Lan Xi-fa2, Wang Yu-lin2, Liu Jing2   

  1. 1Department of Rehabilitation, the Third Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei Province, China;
    2Department of Neurology, First Hospital of Qinhuangdao, Hebei Medical University, Qinhuangdao 066000, Hebei Province, China
  • Received:2012-07-19 Revised:2012-08-10 Online:2012-09-02 Published:2012-09-02
  • Contact: 兰希发，博士，主任医师，教授，河北医科大学附属秦皇岛市第一医院神经内科，河北省秦皇岛市 066000 lanxifa2000@126.com
  • About author:Li Xiao-yun, Associate chief physician, Department of Rehabilitation, the Third Hospital of Qinhuangdao, Qinhuangdao 066000, Hebei Province, China happyxiaoyunli@sohu.com

PDF

459

Abstract:

BACKGROUND: Studies have demonstrated that granulocyte colony-stimulating factor plays an important role in protecting neurons from neuronal degeneration and death caused by a variety of factors.
OBJECTIVE: To investigate the effect of granulocyte colony-stimulating factor on hippocampal neuronal cell apoptosis and Bcl-2 and Bax protein expression in rats with vascular dementia.
METHODS: The permanent bilateral common carotid artery ligation method was used to establish the Sprague-Dawley rat vascular dementia models. Rats with no vascular ligation were included in the sham-operation group. Rats in the treatment group were subcutaneously injected with 50 μg/kg granulocyte colony-stimulating factor daily, rats in the sham-operation group and model group were injected with normal saline. At 7, 14 and 28 days after modeling, rat hippocampus was used for detection.
RESULTS AND CONCLUSION: Morris water maze results showed that the escape latency of the rats in the model group was significantly prolonged (P < 0.01), and the escape latency at different time points in the treatment group was shorter than that in the model group (P < 0.01). TUNEL and immunohistochemistry results showed that, compared with the control group, rat hippocampal TUNEL and Bax positive cell number in the treatment group were significantly reduced (P < 0.01), and the number of Bcl-2 positive cells was significantly increased (P < 0.01). Granulocyte colony-stimulating factor can up-regulate Bcl-2 protein expression in the hippocampus of vascular dementia rats, down-regulate Bax protein expression, reduce neuronal apoptosis and improve the learning and memory abilities of rats.

CLC Number: