Sciatic nerve regeneration in a rat model of brain injury at different locations

doi:10.3969/j.issn.2095-4344.2017.36.012

Abstract

Abstract:

BACKGROUND: Previous studies have shown that traumatic brain injury can promote the regeneration of peripheral nerve by reducing scar collagen in nerve endings.
OBJECTIVE: To investigate the effect of brain injury at different locations on the ipsilateral rat sciatic nerve regeneration.
METHODS: Ninety-nine healthy male Sprague-Dawley rats were equivalently randomized into three groups: group A, right sciatic nerve transection; group B, right sciatic nerve transection combined with right brain injury; and group C, right sciatic nerve transection combined with left brain injury. All of transected nerves were sutured under microscope. Classical Feeney method was used to establish a model of traumatic brain injury. At 4, 6, 8, 10 and 12 weeks after modeling, the sciatic functional index (SFI) was calculated by measuring footprint. At 4, 8 and 12 weeks after modeling, the bilateral gastrocnemius were harvested for determining wet weight and calculate wet weight ratio, followed by acetylcholinesterase staining at the motor end plate to detect the absorbance values. At 4, 8 and 12 weeks after modeling, fluoro-gold retrograde tracing was used to trace L4-5 vertebrae for 1 week, and the number of spinal cord anterior horn motor neurons positive for fluoro-gold was detected and calculated by fluorescence microscope.
RESULTS AND CONCLUSION: The SFI value in each group was gradually improved with time. The SFI value was significantly higher in the groups B and C than the group A at 4 and 6 weeks after modeling (P < 0.05), and was further improved in the group B at 8 weeks compared with the groups A and C (P < 0.05). The wet weight ratio of gastrocnemius showed no significant difference among groups at 4 weeks after modeling (P > 0.05), and the group B showed a significantly higher wet weight ratio than the other groups from the 8th week (P < 0.05). Compared with the groups A and C, the absorbance values of motor endplate in group B appeared to be a significant increase at the beginning of the 8th week (P < 0.05). At 4 and 6 weeks after modeling, the number of spinal cord anterior horn motor neurons positive for fluoro-gold was significantly nigher in the groups B and C than in the group A, and the number was significantly higher in the group B than the groups A and C at 12 weeks (all P < 0.05). These finding manifest that brain injury can promote the repair of ipsilateral sciatic nerve injury, thus proving theoretical reference for unveiling the mechanism by which traumatic brain injury promotes peripheral nerve regeneration.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Brain Injuries, Peripheral Nerves, Nerve Regeneration, Tissue Engineering

CLC Number:

R318

Ma Jian-jun1, He Xin-ze2, Wang Hao-qi1, Sun Bo1, Gao Yun-feng1, Fu Shi-jie1, Wang Pei1. Sciatic nerve regeneration in a rat model of brain injury at different locations[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(36): 5806-5811.

Figures/Tables 3

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