Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (29): 4600-4604.doi: 10.3969/j.issn.2095-4344.2017.29.002

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Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem cells

Chen Yu-shu1, Zhang Yan-hong2, Zhang Shu-jiang1, Bai Bo1, Chen Yi1   

  1. 1Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China; 2Department of Orthopaedic Surgery, Shunde Hospital of Southern Medical University, Shunde 528300, Guangdong Province, China
  • Revised:2017-05-04 Online:2017-10-18 Published:2017-11-08
  • Contact: Bai Bo, Chief physician, Professor, Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China
  • About author:Chen Yu-shu, Department of Orthopaedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong Province, China

Abstract:

BACKGROUND: Parathyroid hormone can activate a series of physiological and biochemical reactions through specific receptors on the surface of target organs, to adjust the dynamic calcium balance.
OBJECTIVE: To explore the mechanisms whereby parathyroid hormone maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs), by observing the effects of parathyroid hormone on calcium ions in BMSCs.
METHODS: BMSCs at passage 4 were randomly divided into two groups. Experimental group was treated with parathyroid hormone, and control group was treated with routine culture medium. During 10 days of culture, MTT assay was used to detect BMSCs proliferation. At 7 and 14 days after treatment, laser scanning confocal microscopy was used to detect fluorescence intensity of calcium ions in BMSCs in the two groups.
RESULTS AND CONCLUSION: (1) At 1 and 2 days after culture, the cell viability in the two groups was sluggish. At 3 days after culture, the cells turned into the rapid growth. At 7 and 8 days after culture, the cell growth turned into the plateau. At 10 days after culture, the cell proliferation ability had a weakening trend, which was lower in the control group than in the experimental group (P < 0.05). (2) At 7 days after culture, the fluorescence intensity of intracellular calcium ions was strong. As the induction time extended, the fluorescence intensity of intracellular calcium ions was declined at 14 days after-culture. At 7 and 14 days after culture, the fluorescence intensity of intracellular calcium ions in the experimental group was higher than that in the control group (P < 0.001). There was no difference in the fluorescence intensity of intracellular calcium ions between experimental group at 14 days and control group at 7 days. To conclude, parathyroid hormone could increase the fluorescence intensity of calcium ions in BMSCs, and function to maintain the BMSCs proliferation.  

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Mesenchymal Stem Cells, Teriparatide, Cell Proliferation, Tissue Engineering

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