Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (24): 3870-3874.doi: 10.3969/j.issn.2095-4344.2017.24.017

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Migration and invasion abilities of normal fibroblast-like synoviocytes versus fibroblast-like synoviocytes in rheumatoid arthritis

Hu Jun1, 2, Tang Zi-zheng1, 3, Zhang Yu1, Shen Wei-gan1, Liu Gui-ping1, Wang Wen-wen1, Liu Jia-huan1   

  1. 1Department of Rheumatology and Immunology, Clinical Medical College of Yangzhou University (North Jiangsu People’s Hospital of Jiangsu Province), Yangzhou 225001, Jiangsu Province, China; 2Department of Internal Medicine, Jiangsu Provincial General Hospital of Armed Police Force, Yangzhou 225001, Jiangsu Province, China; 3Department of Rheumatology, the Fourth People’s Hospital of Jinan, Jinan 250031, Shandong Province, China
  • Revised:2017-03-19 Online:2017-08-28 Published:2017-08-30
  • Contact: Zhang Yu, Master, Professor, Chief physician, Department of Rheumatology and Immunology, Clinical Medical College of Yangzhou University (North Jiangsu People’s Hospital of Jiangsu Province), Yangzhou 225001, Jiangsu Province, China
  • About author:Hu Jun, Attending physician, Department of Rheumatology and Immunology, Clinical Medical College of Yangzhou University (North Jiangsu People’s Hospital of Jiangsu Province), Yangzhou 225001, Jiangsu Province, China; Department of Internal Medicine, Jiangsu Provincial General Hospital of Armed Police Force, Yangzhou 225001, Jiangsu Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81470070

Abstract:

BACKGROUND: Fibroblast-like synoviocytes (FLS) in the synovial lining layer are related to the cell proliferation, invasion, migration and apoptosis as well as bone resorption in rheumatoid arthritis.

OBJECTIVE: To compare the migration and invasion abilities of FLS (MH7A) in rheumatoid arthritis and normal FLS (HFLS).
METHODS: The capacities of cell migration and invasion were evaluated by Transwell cell migration and invasion assays. The primers of the indicated microRNAs were designed and synthesized, and the expression levels of miRNAs were determined by real-time PCR according to the SYBR®PrimeScript™miRNA RT-PCR Kit instruction.

RESULTS AND CONCLUSION: MH7A possessed stronger migration and invasion abilities than HFLS. Compared with HFLS, obviously upregulated miR-132, -155, -203, -223 and -124, and significantly downregulated miR-15a, -16, 18a, -19a, -26a and -146a were found in MH7A. These findings suggest that the differentially expressed 11 kinds of rheumatoid arthritis-associated miRNAs participate in the pathogenesis of rheumatoid arthritis probably by enhancing the migration and invasion capacities of MH7A.

 

Key words: Arthritis Rheumatoid, Cell Movement, MicroRNAs, Tissue Engineering

CLC Number: