Angiogenesis of dermal scaffold transplantation zone promoted by moboilizing endothelial progenitor cells

doi:10.3969/j.issn.2095-4344.2013.24.003

Abstract

Abstract:

BACKGROUND: In recent years, erythropoietin is found to mobilize autologous vascular endothelial progenitor cells from the bone marrow to peripheral blood, which are moved to the wound and promote wound angiogenesis and wound healing ultimately.
OBJECTIVE: To observe the effect of erythropoietin on the mobilization of vascular endothelial progenitor cells.
METHODS: The vascular endothelial progenitor cells were mobilized by intraperitoneal injection of erythropoietin with different concentrations for 7 days, and the cells injected with normal saline were as control. The number of endothelial progenitor cells in the peripheral blood was detected with flow cytometry to compare the mobilization effect. The safe and effective erythropoietin mobilization dose was selected to observe the effect of erythropoietin- mobilized endothelial progenitor cells on the angiogenesis of the dermal scaffold transplantation zone.
RESULTS AND CONCLUSION: In 1, 3, 5, 7, 10 and 14 days after injection, there was no significant change in the number of endothelial progenitor cells in the peripheral blood of the normal saline group, while the number of endothelial progenitor cells in the peripheral blood of the erythropoietin mobilization group was increased gradually and reached peak at 7 days after injection. The proportion of endothelial progenitor cells was significantly increased in the high-dose erythropoietin mobilization group when compared with the low-dose erythropoietin mobilization group. The erythropoietin mobilization group had better effect on the angiogenesis in the dermal scaffold transplantation zone than the control group. Consecutive intraperitoneal injection of erythropoietin for 7 days can increase the number of endothelial progenitor cells in the peripheral blood that reaches peak at 7 days after mobilization. The mobilization effect is in a dose-dependent manner, and the injection can promote the angiogenesis in the dermal scaffold transplantation area.

Key words: tissue construction, vascular tissue construction, endothelial progenitor cells, erythropoietin, acellular porcine dermis, dermal scaffold, angiogenesis, tissue-engineered skin, National Natual Science Foundation of China

CLC Number:

R318

Zhang Wei, Cai Di-xin, Wang Hong, Zhu Yong-cui. Angiogenesis of dermal scaffold transplantation zone promoted by moboilizing endothelial progenitor cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4386-4393.

Figures/Tables 6

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