Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (1): 110-114.doi: 10.3969/j.issn.2095-4344.2017.01.020

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Feasibility of renal tissue stem cell therapy for acute renal failure

Hu Yue-shi, Li Peng, Cao Zhi-hua, Liu Lei, Wang Yang   

  1. Department of Urinary Surgery, Nanyang City Center Hospital, Nanyang 473000, Henan Province, China
  • Online:2017-01-08 Published:2017-03-15
  • About author:Hu Yue-shi, Master, Attending physician, Department of Urinary Surgery, Nanyang City Center Hospital, Nanyang 473000, Henan Province, China

Abstract:

BACKGROUND: Studies have shown that renal tissue stem cells mainly exist in the renal papillae, holding similar characteristics to mesenchymal stem cells, yet it is unknown whether they are involved in the renal repair of tubular epithelial injury.
OBJECTIVE: To explore the effect and feasibility of renal tissue stem cells in the repair of acute renal failure.
METHODS: Renal tissue stem cells were isolated and culture from the renal tissue of 10 Sprague-Dawley rats. Another 30 Sprague-Dawley rats were randomly assigned into experimental group, model group or normal control group. An animal model of acute renal failure was made in the experimental and model groups. Six hours after modeling, experimental rats were respectively given tail vein injection of renal tissue stem cells in the experimental group, given the same volume of normal saline in the model group, and given no treatment in the normal control group. Ten days after transplantation, the renal function was detected, and renal tissue samples were taken and observed histologically.
RESULTS AND CONCLUSION: After transplantation, levels of urea nitrogen and creatinine were improved significantly in the experimental group (P < 0.05), which were similar to those in the normal control group (P > 0.05). No abnormal changes and no inflammatory infiltration in the cortex and outer medullary tubules were found in the normal control group, while in the experimental group, inflammatory cell infiltration and epithelial cell swelling were visible but milder than the model group. To conclude, our experimental findings show that the renal tissue stem cells get ideal effects on the renal function of acute renal failure rats by promoting epithelial cell regeneration.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Kidney Tubular Necrosis, Acute, Acute Kidney Injury, Tissue Engineering

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