Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (47): 7064-7069.doi: 10.3969/j.issn.2095-4344.2016.47.010

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Effect of polytetrafluoroethylene artificial blood vessels on platelet aggregation and its cytocompatibility

Jiang Zheng-ming, Chen Kui, Hu Cai-na
  

  1. Department of Cardiovasology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Received:2016-08-22 Online:2016-11-18 Published:2016-11-18
  • Contact: Hu Cai-na, Department of Cardiovasology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • About author:Jiang Zheng-ming, Master, Attending physician, Department of Cardiovasology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Supported by:
    the Project of Henan Province Department of Education, No. 142300410356

Abstract:

BACKGROUND: Polytetrafluoroethylene artificial blood vessels have been extensively used in clinic. However, there is still a lack of basic study on it.
OBJECTIVE: To observe the effect of polytetrafluoroethylene artificial blood vessels on platelet aggregation and its cytocompatibility.
METHODS: Effect on platelet aggregation: Dynamic platelet clotting time and plasma recalcification time were detected. Normal saline, distilled water and polytetrafluoroethylene artificial blood vessels were added into the rabbit red cell suspensions, respectively, to count the hemolysis ratio. Cytocompatibility: Primary myocardial cells from Sprague-Dawley rats were respectively seeded onto the common round coverslip and polytetrafluoroethylene artificial blood vessels, and the lactate deshydrogenase release and cell proliferation were detected at 3 and 5 days, respectively.
RESULTS AND CONCLUSION: Polytetrafluoroethylene artificial blood vessels made no effects on the platelet clotting time and plasma recalcification time and 2.3% hemolysis ratio was observed. Compared with the common round coverslip, the myocardial cell proliferation and lactate deshydrogenase release showed no significant changes on the polytetrafluoroethylene artificial blood vessels. These results suggest that the polytetrafluoroethylene artificial blood vessel makes no effect on platelet aggregation and holds good hemocompatibility and cytocompatibility.

Key words: Polytetrafluoroethylene, Blood Vessels Prosthesis, Platelet Aggregation, Tissue Engineering

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