Neural stem cell transplantation for the treatment of rat spinal cord injury in recovery period

doi:10.3969/j.issn.2095-4344.2013.19.014

Abstract

Abstract:

BACKGROUND: Studies have found that extracellular signal-regulated kinase can up-regulate the reactive glial cells after spinal cord injury, and the number of phosphorylated extracellular signal-regulated kinase is increased.
OBJECTIVE: To evaluate the effect of neural stem cell transplantation on motor function recoveryof rats with spinal cord injury in the recovery period, and to explore the focal differentiation and mechanism.
METHODS: Ninety Sprague Dawley rats were randomly divided into three groups, 30 rats in each group: control group, local transplantation group and intravenous infusion group. Rats in the control group were used to establish the models but did not receive neural stem cell transplantation; rats in the local transplantation group and the intravenous infusion group received neural stem cell transplantation after modeling. Six rats were collected from each group; then Basso, Beattie and Bresnahan score and immunohistochemical staining were performed at 1, 2, 4, 12 and 24 weeks after transplantation.
RESULTS AND CONCLUSION: After neural stem cell transplantation, Brdu positive cells could be seen in local lesions of local transplantation group and the intravenous infusion group; Basso, Beattie and Bresnahan score at 4 weeks after transplantation in the local transplantation group and the intravenous infusion group was significantly higher than that in the control group; levels of rat spinal cord glial fibrillary acidic protein and extracellular signal-regulated kinase 1/2 were gradually increased and reached peak at 4 weeks after transplantation; at 12 weeks after transplantation, the levels of spinal cord glial fibrillary acidic protein and extracellular signal-regulated kinase 1/2 were decreased (P < 0.05); the level of microtubule-associated protein 2 was increased and reached peak at 4 weeks after transplantation, and gradually decreased after 4 weeks; at 12 weeks after transplantation, there was no significant difference in microtubule-associated protein 2 level, but the microtubule-associated protein 2 level was higher than that in the control group (P < 0.01). After neural stem cell transplantation, the neural stem cells could proliferate and differentiate into neuron and neuroglial cells. There is no significant difference between the two methods. There may be a certain correlation between the glial cells regeneration process and extracellular signal-regulated kinase pathways.

Key words: stem cells, stem cell transplantation, neural stem cells, glial fibrillary acidic protein, extracellular signal-regulated kinase 1/2, microtubule-associated protein 2, spinal cord injury, neurons, glial cells, extracellular signal-regulated kinase pathway, provincial grants-supported paper, stem cell photographs-containing paper

CLC Number:

R394.2

Li Zhe, Shen Li-fang, Wang Guo-sheng, Guo Gang-hua. Neural stem cell transplantation for the treatment of rat spinal cord injury in recovery period[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(19): 3508-3514.

Figures/Tables 5

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