Effects of mesenchymal stem cells conditioned media on the transforming growth factor-beta signaling pathway in normal and hypertrophic scar fibroblasts

doi:10.3969/j.issn.2095-4344.2016.29.013

Abstract

Abstract:

BACKGROUND: Numerous studies have shown that mesenchymal stem cells (MSCs) can effectively attenuate the fibrosis of damaged heart, lung and kidney by secreting various bioactive factors.
OBJECTIVE: To evaluate the anti-fibrotic therapeutic potential of bone marrow MSCs conditioned media in vitro.
METHODS: Normal fibroblasts and hypertrophic scar fibroblasts were treated with bone marrow MSCs conditioned media, then transforming growth factor-β and collagen production were analyzed by ELISA, and mRNA expression level of Smad7 and hydroxyproline content were detected by RT-PCR and colorimetry, respectively.
RESULTS AND CONCLUSION: Bone marrow MSCs conditioned media significantly inhibited the production of both transforming growth factor-β and collagen in hypertrophic scar fibroblasts (P < 0. 01), and up-regulated the mRNA expression level of Smad7 (P < 0. 01), a major inhibitory regulator in the SMAD family. However, the normal fibroblasts were scarcely influenced by bone marrow MSCs conditioned media. These findings indicate that bone marrow MSCs conditioned media is considered a promising candidate for the treatment of hypertrophic scars, which may provide new theoretical supports to reduce cutaneous scarring.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Mesenchymal Stem Cells, Cicatrix, Fibroblasts, Collagen, Cell Proliferation

CLC Number:

R318

Wu Yan, Yuan Xiao-huan, Yang Lan, Zhao Xiao-jin, Li Hou-zhong, Wang Ying, Jin Hong. Effects of mesenchymal stem cells conditioned media on the transforming growth factor-beta signaling pathway in normal and hypertrophic scar fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(29): 4349-4354.

Figures/Tables 1

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