Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (2): 210-216.doi: 10.3969/j.issn.2095-4344.2013.02.005
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Zhang Xin-le, Wu Tie, Cui Liao, Xu Bi-lian
Received:
2012-03-11
Revised:
2012-03-30
Online:
2013-01-08
Published:
2013-01-08
Contact:
Wu Tie, Professor, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China CCLE2011@163.com
About author:
Zhang Xin-le★, Master, Lecturer, Department of Pharmacology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China CCLE2011@163.com
Supported by:
Supported by: the National Natural Science Foundation of China, No. 30672470*; Special Funds for Science and Technology Competitive Distribution Program of Zhanjiang City
CLC Number:
Zhang Xin-le, Wu Tie, Cui Liao, Xu Bi-lian. Effects of compound danshen extract on bone histomorphometry of high-fat rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 210-216.
2.1 实验动物数量分析 纳入SD大鼠30只,均进入结果分析,无脱落。 2.2 复方丹参对高脂大鼠血脂的影响 见表1。 从表1结果可见,与正常对照组相比,高脂乳剂组大鼠胆固醇升高794%(P < 0.01),高密度脂蛋白胆固醇降低71%(P < 0.01),三酰甘油水平无明显变化,提示长期灌胃高脂乳剂可导致大鼠出现血清胆固醇升高、高密度脂蛋白胆固醇降低为特征的脂质代谢紊乱,与人类Ⅱa型高脂血症相似。与高脂乳剂组比,复方丹参组大鼠胆固醇降低35.9%(P < 0.05)、高密度脂蛋白胆固醇升高100%(P < 0. 01),三酰甘油水平无明显变化,提示复方丹参可有效改善高脂乳剂导致的脂质代谢紊乱"
由表2,3可知:与正常对照组相比,高脂乳剂组的骨小梁数量明显减少,结构稀疏、断裂;骨小梁面积分数、骨小梁数目分别减少了39.6%,29.8%(P < 0.05),骨小梁分离度增加了80.7%(P > 0.05),而每毫米破骨细胞数与%破骨细胞周长百分率分别增加了402%和515%(P < 0.01),成骨骨细胞周长百分率减少了16.8% (P > 0.05);说明高脂已使大鼠松质骨的骨小梁显著减少,微观结构疏松退化,且对破骨细胞的数量及活性有促进作用,而对成骨细胞有抑制作用。与高脂乳剂组相比,复方丹参组的骨小梁明显增多、增粗,连续性良好;复方丹参使高脂大鼠胫骨上段的骨小梁面积百分数、骨小梁数目分别升高了69.8%、56.4%(P < 0.01),骨小梁分离度减少了57.5%(P < 0.01);每毫米破骨细胞数和破骨细胞周长百分率分别减少了79.0%和76.8%(P < 0.01),成骨细胞周长百分率增加了117%(P < 0.01)。说明复方丹参可有效预防高脂对大鼠胫骨松质骨显微结构的破坏,对破骨细胞的数量及活性均有明显的抑制作用,对成骨细胞的数量及活性均有明显的促进作用。"
由表5可知,与正常对照组相比,高脂乳剂组大鼠骨髓腔扩大,皮质骨面积比例下降;静态指标皮质骨面积百分数减少4.4%(P < 0.01),骨髓腔面积百分数增加15.1%(P < 0.01),提示高脂使大鼠胫骨中段皮质骨面积比例下降,骨髓腔变大,说明高脂可引起大鼠皮质骨骨量丢失。与高脂乳剂组相比,复方丹参组的皮质骨面积百分数增加4.9%(P < 0.05)、骨髓腔面积百分数减少13.9%(P < 0.05),提示复方丹参可有效预防D-半乳糖引起的大鼠胫骨中段皮质骨骨量丢失。由表6可知,与正常对照组相比,高脂乳剂组大鼠皮质骨骨外膜的荧光周长百分数明显减少(P < 0.01),其他内外膜的动态参数无明显变化(P > 0.05),提示高脂会减少大鼠皮质骨外膜的成骨数量,但对骨内外膜的骨形成和矿化作用均无明显作用。与高脂乳剂组相比,复方丹参组除骨内膜荧光周长百分数明显减少外其他骨内外膜的动态参数无明显变化,提示复方丹参并不能促进大鼠皮质骨骨内外膜的骨矿化和骨形成作用。"
高脂乳剂组大鼠腰椎松质骨骨量明显减少,复方丹参组有一定的预防作用,见图3。 由表7,8,图3可知:与正常对照组相比,高脂乳剂组骨小梁数量明显减少,结构稀疏、断裂;骨小梁数量明显减少,结构稀疏、断裂;骨小梁面积百分数、骨小梁宽度分别减少了32.4%(P < 0.01)、22.1% (P < 0.05),骨小梁分离度增加了40.4%(P < 0.05),而每毫米破骨细胞数、破骨细胞周长百分率、成骨骨细胞周长百分率无明显变化;说明高脂已使大鼠松质骨的骨小梁显著减少,微观结构疏松退化,但对细胞没有明显的促进或抑制作用。与高脂乳剂组相比,复方丹参组骨小梁明显增多、增粗,连续性良好;复方丹参使高脂大鼠胫骨上段的骨小梁面积百分数、骨小梁数目、骨小梁宽度分别升高了36.8%,123.5%,12.2%(P < 0.05),骨小梁分离度减少了28.8%(P < 0.01);因高脂乳剂组较正常对照组的细胞无明显变化所以不继续测量复方丹参组每毫米破骨细胞数、破骨细胞周长百分率、成骨骨细胞周长百分率值。静态参数的变化说明复方丹参可有效预防高脂对大鼠第5腰椎松质骨显微结构的破坏。 由表9可知,与正常对照组相比,高脂乳剂组5个主要动态参数指标无明显变化趋势,说明长期高脂乳剂灌胃对大鼠第5腰椎反映骨形成及骨矿化指标变化不明显,因而亦不继续测量复方丹参组。"
[1] Xu J, Zhao DB, Liu WB. Zhongguo Quanke Yixue. 2010; 13(11): 1246-1248.许洁,赵东宝,刘文斌.老年性骨质疏松症的防治进展[J].中国全科医学,2010,13(11):1246-1248.[2] Chen GF, Zhang LH, Chen ZJ. Fangshe Mianyixue Zazhi. 2009;22(5):489-490.陈国富,张腊红,陈兆军.骨质疏松症患者血清骨钙素与血脂的相关性分析[J].放射免疫学杂志,2009,22(5):489-490.[3] You TT, Wu T, Zhang ZP, et al. Zhongguo Linchuang Yaolixue yu Zhiliaoxue. 2007;12(3):299-303.尤婷婷,吴铁,张志平,等.高脂血症致大鼠骨质疏松作用研究初探[J].中国临床药理学与治疗学,2007,12(3):299-303.[4] Zhang LH, Huang GM. Zhongguo Shiyong Yixue. 2010;5(18): 74-75.张俐虹,黄国民.复方丹参滴丸对高血压患者血脂代谢及CRP 的影响[J].中国实用医药,2010,5(18):74-75.[5] Feng JB, Chen HY, Sun W. Shandong Yiyao. 2009;49(9):43.冯静波,陈洪颖,孙伟.复方丹参滴丸对高脂血症患者血脂水平的影响[J].山东医药,2009,49(9):43.[6] Li XP, Zhao SP, Qin YN. Zhongxiyi Jiehe Xinnaoxueguanbing Zazhi. 2010;8(11):1283-1285.李向平,赵水平,秦英楠.复方丹参滴丸对冠心病及其高危患者血脂及大动脉弹性的影响[J].中西医结合心脑血管病杂志,2010, 8(11): 1283-1285.[7] Chinese Pharmacopoeia Commission. Chemical Industry Press. 2000:518.国家药典委员会编.中华人民共和国药典(2000年版一部)[S].北京:化学工业出版社,2000:518.[8] Zhang XL, Wu T, Cui L, et al. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 2010;14(28):5141-5145.张新乐,吴铁,崔燎,等.复方司坦唑醇对D-半乳糖大鼠不同部位骨骼的影响[J].中国组织工程研究与临床康复,2010,14(28): 5141-5145.[9] Zhang ZP, You TT, Zou LY, et al. Nanfang Yike Daxue Xuebao. 2008;28(9):550-1553.张志平,尤婷婷,邹丽宜,等.复方丹参合剂对高脂血症性骨质疏松小鼠血脂、骨生物力学的影响[J].南方医科大学学报,2008,28(9): 1550-1553.[10] Parhami F, Garfinkel A, Demer LL. Role of lipids in osteoporosis. Arterioscler Thromb Vasc Biol. 2000;20(11):2346-2348.[11] Kajkenova O, Lecka-Czernik B, Gubrij I, et al. Increased adipogenesis and myelopoiesis in the bone marrow of SAMP6, a murine model of defective osteoblastogenesis and low turnover osteopenia. J Bone Miner Res. 1997;12(11):1772-1779.[12] Sakaguchi K, Morita I, Murota S. Relationship between the ability to support differentiation of osteoclast like cells and adipogenesis in murine stromal cells derived from bone marrow. Prostaglandins Leukot Essent Fatty Acids. 2000; 62(5): 319-327.[13] Zhang MY, Yuan XG, Meng FC. Zhongguo Shiyong Shenjing Jibing Zazhi. 2011;14(4):75-76.张明艳,袁小庚,孟凡超.洛伐他汀联合复方丹参滴丸治疗高脂血症临床观察[J].中国实用神经疾病杂志,2011,14(4):75-76.[14] Liu XY, Zhou L, Wang PX. Zhonghua Zhongyiyao Xuekan. 2011;29(2):369-371.刘新迎,周联,王培训.以3T3-L1细胞模型探讨部分丹参提取物的调脂机制[J].中华中医药学刊,2011,29(2):369-371.[15] Liu HY, Tian S. Yaowu yu Linchuang. 2009;47(24):124-125.刘红玉,田爽.复方丹参滴丸对高血脂患者血液流变学的影响[J].药物与临床,2009,47(24):124-125.[16] Tang ZL, Zhang HP, Li Y, et al. Hubei Zhongyi Zazhi. 2008; 30(6):11-12.汤治黎,章汉平,李源,等.丹参注射液诱导大鼠骨髓间充质干细胞分化为成软骨细胞的研究[J].湖北中医杂志,2008,30(6):11-12. |
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