Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (29): 4290-4296.doi: 10.3969/j.issn.2095-4344.2016.29.005

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Association between single-nucleotide polymorphisms in interleukin-12 and susceptibility to osteoarticular tuberculosis in Guangxi Zhuang population

Lu Lu1, Wei Gui-jiang2, Xie Ke-gong1, Wang Jun-li2, Lin Jia-jie1, Qiao Ning-ning1, Tang Yu-jin1   

  1. 1Department of Spine Surgery, 2Laboratory Department, First Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Online:2016-07-08 Published:2016-07-08
  • Contact: Tang Yu-jin, Professor, Chief physician, Master’s supervisor, Department of Spine Surgery, First Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • About author:Lu Lu, Master, Attending physician, Department of Spine Surgery, First Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81541136; a grant from Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, China, No. Z2012712; Scientific Research and Technological Development Program of Baise, China, No. 20160610, 20140931

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Abstract:

BACKGROUND: Interleukin-12 (IL-12) may function as an immune regulator in the pathogenesis of osteoarticular tuberculosis.
OBJECTIVE: To explore the association of single-nucleotide polymorphisms in IL-12A rs568408 G/A and IL-12B rs3212227 A/C with susceptibility to osteoarticular tuberculosis and serum interleukin-12 levels in Guangxi Zhuang population.
METHODS: The single-nucleotide polymorphisms in IL-12A rs568408 G/A and IL-12B rs3212227 A/C polymorphisms were detected by polymerase chain reaction-single base extension technique and direct DNA sequencing in 150 patients with osteoarticular tuberculosis (disease group) and 165 healthy individuals (control group) in Guangxi Zhuang population. The genotype and allele frequencies of IL-12 and the relationship of genotypes to the susceptibility to osteoarticular tuberculosis were analyzed. In addition, the association of genotypes of single-nucleotide polymorphisms in IL-12A rs568408 G/A and IL-12B rs3212227 A/C with serum IL-12 levels were analyzed.
RESULTS AND CONCLUSION: There was no significant difference in the genotype and allele frequencies of IL-12A rs568408 G/A and IL-12B rs3212227 A/C between the disease group and the control group (P > 0.05). Moreover, there was no difference in four haplotypes of IL-12 gene between the disease group and the control group (P > 0.05). Serum IL-12 levels in subjects with osteoarticular tuberculosis carrying the variant rs568408 GA/AA genotypes and wild-type rs568408 GG genotypes were similar (P > 0.05). Similarly, there was no significant difference in serum IL-12 levels between subjects with osteoarticular tuberculosis carrying the variant rs3212227 AC/CC genotypes and wild-type rs3212227 AA genotypes (P > 0.05). These findings suggest that the single-nucleotide polymorphisms in IL-12A rs568408 G/A and IL-12B rs3212227 A/C polymorphisms are not associated with susceptibility to osteoarticular tuberculosis in Guangxi Zhuang population.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tuberculosis, Osteoarticular, Interleukin-12, Genes, Polymorphism, Single Nucleotide, Tissue Engineering

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