Influence of interleukin-1beta on microRNA-27b and matrix metalloproteinase-13 expression in rat chondrocytes

doi:10.3969/j.issn.2095-4344.2016.29.003

Abstract

Abstract:

BACKGROUND: Matrix metalloproteinase-13 is most active in the degradation of collagen type II in the extracellular matrix of cartilage. Interleukin-1 (IL-1) is thought to be the inducer of matrix metalloproteinases, and participates in the degradation and degeneration of articular cartilage.
OBJECTIVE: To study the influence of IL-1β on microRNA-27b (miR-27b) and matrix metalloproteinase-13 expression of chondrocytes in rats.
METHODS: Chondrocytes isolated from seven male Wistar rats were cultured and divided into IL-1β stimulation group and control group. No stimulus was given in the control group; 10 μg/L of serum free medium was used to culture rat chondrocytes in the IL-1β stimulation group. Cell growth was observed at 0, 24, and 48 hours under an inverted microscope. miR-27b and matrix metalloproteinase-13 expression in the cultured chondrocytes were detected.
RESULTS AND CONCLUSION: The relative expression of matrix metalloproteinase-13 in rat chondrocytes was gradually increased when induced by IL-1β at 0, 24, and 48 hours (P < 0.05). Expression of miR-27b and miR-31 in rat chondrocytes at 24 and 48 hours induced by IL-1β gradually decreased (P < 0.05); conversely, expression of MiR-26a, miR-26b, miR-23, and miR-204 gradually increased (P < 0.05). After 48 hours of IL-1β induction, expression of miR-27b was the lowest in rat chondrocytes (P < 0.05). These findings suggest that IL-1β inhibits miR-27b expression, strengthens the expression of matrix metalloproteinase-13, and damages chondrocytes, contributing to both the onset and progression of osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Interleukins, Matrix Metalloproteinases, MicroRNAs, Osteoarthritis

CLC Number:

R318

Shi Jing, Li Tong, Zuo Can-hui, Zhi Liang, Wei Rong, Wang Wei-min, Liu Ying-jie. Influence of interleukin-1beta on microRNA-27b and matrix metalloproteinase-13 expression in rat chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(29): 4277-4283.

Figures/Tables 2

References

[1] Ludwig CA, Mobargha N, Okogbaa J, et al. Altered Innervation Pattern in Ligaments of Patients with Basal Thumb Arthritis. J Wrist Surg.2015;4(4): 284-291.
[2] Yu SP, Hunter DJ. Managing osteoarthritis. Aust Prescr.2015,38(4):115-119.
[3] Feng J, Xia Y, Yuan L, et al. An increased level of interleukin 27 in peripheral blood mononuclear cells and fibroblasts like synoviocytes of patients with rheumatoid arthritis or osteoarthritis. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi.2015;1(12):1673-1676.
[4] Shi T, Gao G, Yan Z, et al. Changes of six serum biomarkers of rabbits with experimental osteoarthritis. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi.2015;31(12): 1620-1623.
[5] Tanaka R, Ozawa J, Kito N, et al. Does exercise therapy improve the health-related quality of life of people with knee osteoarthritis? A systematic review and meta-analysis of randomized controlled trials. J Phys Ther Sci.2015;27(10):3309-3314.
[6] Tsonga T, Michalopoulou M, Malliou P, et al. Analyzing the History of Falls in Patients with Severe Knee Osteoarthritis. Clin Orthop Surg.2015;7(4):449-456.
[7] Østerås N, van Bodegom-Vos L, Dziedzic K, et al. Implementing international osteoarthritis treatment guidelines in primary health care: study protocol for the SAMBA stepped wedge cluster randomized controlled trial. Implement Sci.2015;10(1):165.
[8] Wright TW, Grey SG, Roche CP, et al. Preliminary Results of a Posterior Augmented Glenoid Compared to an all Polyethylene Standard Glenoid in Anatomic Total Shoulder Arthroplasty. Bull Hosp Jt Dis (2013). 2015;73(1):79-85.
[9] 陈铖,马翅,张莹,等.AGEs对兔软骨细胞TNF-α和MMP-13表达的影响及其机制研究[J].湖南师范大学学报医学版,2013,(4):23-28.
[10] Jiang W,Lei G,Lin B,et al.Effect of osteopontin on expression of matrix metalloproteinase 13 in human knee osteoarthritic chondrocytes.Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi.2014;28(11):1342-1345.
[11] 付婷,王锁良.川芎嗪对体外培养兔关节软骨细胞MMP-1、MMP-13和TIMP-1表达的影响[J].西安交通大学学报:医学版,2012,33(1):114-117.
[12] 赵敬河,杨秋云,王冰,等.骨关节炎时内源性细胞因子及基质金属蛋白酶抑制因子1对关节软骨的影响[J].中国组织工程研究,2012,16(15):2665-2668.
[13] Wu D,Huang P,Wang L,et al.MicroRNA-143 inhibits cell migration and invasion by targeting matrix metalloproteinase 13 in prostate cancer.Mol Med Rep.2013;8(2):626-630.
[14] 王欣,秦宇.白细胞介素-1β对大鼠软骨细胞MMP-13表达的影响及miR-27b的调控作用[J].天津医药,2015,(8): 871-875.
[15] Attur M, Statnikov A, Samuels J, et al. Plasma levels of interleukin-1 receptor antagonist (IL1Ra) predict radiographic progression of symptomatic knee osteoarthritis. Osteoarthritis Cartilage.2015;23(11): 1915-1924.
[16] Min Z, Zhang R, Yao J, et al. MicroRNAs associated with osteoarthritis differently expressed in bone matrix gelatin (BMG) rat model. Int J Clin Exp Med.2015,8(1): 1009-1017.
[17] Trzeciak T, Czarny-Ratajczak M. MicroRNAs: Important Epigenetic Regulators in Osteoarthritis. Curr Genomics.2014;15(6):481-484.
[18] Jingsheng S, Yibing W, Jun X,et al. MicroRNAs are potential prognostic and therapeutic targets in diabetic osteoarthritis. J Bone Miner Metab.2015;33(1):1-8.
[19] Palladini G,Ferrigno A,Richelmi P,et al.Role of matrix metalloproteinases in cholestasis and hepatic ischemia/reperfusion injury:A review.World J Gastroenterol.2015;21(42):12114-12124.
[20] Li BT,Zhang FZ,Xu TS,et al.Increasing production of matrix metalloproteinases, tumor necrosis factor-α,vascular endothelial growth factor and prostaglandin E2 in rheumatoid arthritis synovial fibroblasts by different adiponectin isoforms in a concentration-dependent manner.Cell Mol Biol (Noisy-le-grand).2015;61(7):27-32.
[21] 田华,张松峰,芦琨,等.川芎嗪对CIA大鼠足爪组织基质金属蛋白酶13蛋白表达及血清VEGF、IL-17和IL-23水平的影响[J].中国病理生理杂志,2013,29(7):1302-1306.
[22] Zeng GQ, Chen AB, Li W, et al. High MMP-1, MMP-2, and MMP-9 protein levels in osteoarthritis. Genet Mol Res.2015;14(4):14811-14822.
[23] Xia LU, He H, Guo H, et al. Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits. Exp Ther Med.2015;10(4):1429-1436.
[24] Santoro A, Conde J, Scotece M, et al.SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways. PLoS One.2015;10(8):e0135979.
[25] Liu Z, Cai H, Zheng X, et al. The Involvement of Mutual Inhibition of ERK and mTOR in PLCγ1-Mediated MMP-13 Expression in Human Osteoarthritis Chondrocytes. Int J Mol Sci.2015;16(8):17857-17869.
[26] Wang L, Ma T, Zheng Y, et al. Diosgenin inhibits IL-1β-induced expression of inflammatory mediators in human osteoarthritis chondrocytes. Int J Clin Exp Pathol. 2015;8(5):4830-4836.
[27] Wang D, Qiao J, Zhao X, et al. Thymoquinone Inhibits IL-1β-Induced Inflammation in Human Osteoarthritis Chondrocytes by Suppressing NF-κB and MAPKs Signaling Pathway. Inflammation.2015;38(6):2235-2241.
[28] Uchimura T, Foote AT, Smith EL, et al. -----Insulin-Like Growth Factor II (IGF-II) Inhibits IL-1β-Induced Cartilage Matrix Loss and Promotes Cartilage Integrity in Experimental Osteoarthritis. J Cell Biochem.2015; 116(12):2858-2869.
[29] Zhu W,Zou B,Nie R,et al. A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor γ with miR-27 involved mechanism.J Nutr Biochem.2015; 26(11):1124-1135.
[30] Nianhu L, Lei X, Sheng Y, et al. Effect of Bushenhuoxue formula on interleukin-1 beta and discoidin domain receptor 2 levels in a rat model of osteoarthritis.J Tradit Chin Med.2015;35(2):192-196.
[31] Yang L, Zhang J, Wang G. The effect of sodium hyaluronate treating knee osteoarthritis on synovial fluid interleukin -1β and clinical treatment mechanism.Pak J Pharm Sci.2015;28(1):407-410.
[32] Wu C, Tian B, Qu X, et al. MicroRNAs play a role in chondrogenesis and osteoarthritis (review) . Int J Mol Med.2014;34(1):13-23.
[33] Bernard NJ. Osteoarthritis: circulating miRNAs-early osteoarthritis biomarkers?. Nat Rev Rheumatol.2014; 10(4):197.
[34] Beyer C, Zampetaki A, Lin NY, et al. Signature of circulating microRNAs in osteoarthritis. Ann Rheum Dis.2015;74(3):e18.
[35] 安新,高利常,孙太起,等.IL-32、MMP-13及IL-10在类风湿关节炎中的表达及意义研究[J].中华临床医师杂志(电子版),2013,(20):9086-9089.
[36] Le LT, Swingler TE, Clark IM. Review: the role of microRNAs in osteoarthritis and chondrogenesis. Arthritis Rheum.2013,65(8):1963-1974.
[37] Miyaki S, Asahara H. Macro view of microRNA function in osteoarthritis.Nat Rev Rheumatol.2012;8(9):543-552.
[38] Nicola JP,Peyret V,Nazar M,et al.S-Nitrosylation of NF-κB p65 Inhibits TSH-Induced Na(+)/I(-) Symporter Expression.Endocrinology.2015;156(12):4741-4754.
[39] Hong E, Reddi AH. MicroRNAs in chondrogenesis, articular cartilage, and osteoarthritis: implications for tissue engineering. Tissue Eng Part B Rev.2012;18(6): 445-453.
[40] Steck E, Boeuf S, Gabler J, et al. Regulation of H19 and its encoded microRNA-675 in osteoarthritis and under anabolic and catabolic in vitro conditions.J Mol Med (Berl).2012;90(10):1185-1195.